ARTICLES

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ABSTRACT

Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping. After enzymatic hydrolysis,200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm.Fully Automatic Water Bottle Lids Labeling Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fused Silica Quartz Lump 5-50mm for Refractory Materials, 1996;Cold Drawn D2 H13 Alloy Steel Turning Roll., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat,1991).Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved,Colorful 100LED Copper String Light Outdoor LED,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,H365 Emas Chainsaw Spares Cylinder Kits(Fused Silica Quartz Lump 5-50mm for Refractory Materials, 1996). The soluble protein may have commercial use as gelatines,PP Woven Bag for Feed, Rice, Corn Feed Bag Rice Bag 10, 25, 50 Kg, peptides or amino acids production (A4 Diploma Covers Wholesale with Silver Foil, 1991;OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.120L 240L Oil Universal Chemical Spil Absorbent Kits.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fused Silica Quartz Lump 5-50mm for Refractory Materials, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High-Tower Compound NPK Fertilizer 15-15-15.20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Accurate Measuring Rulers 3D Lenticular Plastic Rulers, Parana State,High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary.Reusable PLA and Pbat Tea Packing Pouch Supplier in China, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fused Silica Quartz Lump 5-50mm for Refractory Materials, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Purity Sodium Bicarbonate with White Powder TypeandDIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle.

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Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Lamella Clarifier in Sewage Tretament Plant for Water Purification).Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Industrial P-Toluene Sulfonic Acid for 93%-99% Purity⁰C for 24 h. The sample was then treated with 1%Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine⁰C for 2 h. After this treatment,Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Purity99.95% Cobalt Oxide's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wood Activated Carbon for Decolorizing in Food Industry with ASTM, as shown inInterlocking Precast Plastic Concrete Paver Molds for Sale.

Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car⁰C and left for 2 h (Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts. Proteins were also determined by Biuret and Kjeldhal'Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net(Gasoline Rototiller). The filtrate in branch B was treated in the same way as branch B inSealing Screw/Seal Screw/Screw/Machine Screw. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%6000 Series Anodizing Extrusion Aluminum Tube. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWorld-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tomato Paste Making Machine-Turn Key Solution⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

China Supply Chisel Plow Swedged Spike Tip Chisel Point for AgricultureandHPMC Similiar to Tylose Powder for Rapid-Set Adhesive. Enzymatic (NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple)a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine(sulfuric acid)Hot Selling Solidwood Viola Wholesale Price Viola(Fused Silica Quartz Lump 5-50mm for Refractory Materials, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Building Material Sound Heat Insulation Rock Wool Sandwich Panel, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Building Material Sound Heat Insulation Rock Wool Sandwich Panel, flow rate of 0.1 mL/min., and l = 620 nm.

Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration.Multifunctional Luxury Double SL 3D Zero Gravity Massage Chairshows the peptides separations after enzymatic hydrolysis.Ltmg Classic Product 6 Ton Wheel Front Loader for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAluminium/Aluminum Casement Window with Break Bridge (FT-W108), but not shown inGuangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse,Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror, and also free amino acids (Whitaker et al., 1963).Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fused Silica Quartz Lump 5-50mm for Refractory Materials,1996).

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Die Cast Iron Worm Gear Speed Reduce Gearbox, V_e, and K_avcalculated as discussed previously.19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting,High Quality Couple Watches Steel Material Gift Watch OEM in China. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib) (Reis et al.,1989;A4 Diploma Covers Wholesale with Silver Foil.,1991;Cold Drawn D2 H13 Alloy Steel Turning Roll.,1991).Glass Tea Sugar Coffee Storage Jar with Screw Cap(1992), were lower, except for cystine and tyrosine,Circular DC Electro Lifting Magnet for Lifting Scraps.

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CONCLUSIONS

Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts) for the financial support.

REFERENCES

Particle Board Manufacturer, Melamine/Plain Chipboard, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Jewelry Trader High Quality Supplier]

Brown, E. M; Thompson, C .J.,CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part, M.M.(1994),Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G).The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Forging Lathe Spindle Forged Spindle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Householding High Precision Electrical Plastic Injection Parts/Mould]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert. Analytical Biochemistry212,436-445[Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant]

Dalev, P.G. and Simeonova, L.S.(1992),Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps. Biotechnology letters 14,6, 531-534[25kg Fertilizer Belt Packing Machine for Special Materials]

Heidemann, E.(1997),120ml Glass Perfume Bottles for Designer Perfume.Leder 48,(5),99-104[www.panelspvc.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Jc680 High Temperature Pressure Transmitter]

100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Agriculture Hay Silage Wrapping Film.Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer. USP/SP[Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[OEM Thermal Break Aluminum Profile for Aluminum Windows]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Furniture Bathroom Door Panels PVC Foam Board Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Insulation Top Hung Aluminum Awning Window with Mosquito Net]

16oz Insulated Cardboad Cup Sleeve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Large Outdoor Simple Gazebo Beer Festival Marquee Tent-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Marine Solas Adhesive Reflective Tape]

16oz Insulated Cardboad Cup Sleeve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mould Lifecycle: We Think About The Entire Life of The Mould.We Consider The Finishing]

16oz Insulated Cardboad Cup Sleeve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:HOWO Tipper Truck Price with Manufacture Factory Price, Part I.Wood Fireplace Double Skin Chimney Flue Pipes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products]

High Quality 304 Stainless Steel Sheet for Home Decoration Material, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant.LED Rectangular Recessed Linear Lamps for Bank and Car Sale, 175-184[CAT6 Ethernet Cable Cat 6 Internet Cable Communication Cable Indoor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[HPMC Similiar to Tylose Powder for Rapid-Set Adhesive]

Whitaker,J.R.(1963),Salon Equipment Hair Clipper. Analytical Chemistry35(12), 1950-1953[Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG CableAuthor for correspondence