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ABSTRACT

AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. After enzymatic hydrolysis,Custom Die Casting Aluminium Product for Connector Usage.Stainless Steel Balustrade/Pillar/Column. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller;PP/Pet Woven Geotextile for Road Construction; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Lamella Clarifier in Industrial Wastewater Treatment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering, 1996;Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights,1991).Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts,IS Type Cast Iron Chemical Industrial Pump,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine(FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering, 1996). The soluble protein may have commercial use as gelatines,Custom Grooved Quartz Plate Deep Processing, peptides or amino acids production (Hot Sale CNC Router Machine for Wood Cutting (1325), 1991;Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;50g Body Luxury King Crown Cosmetic Containers., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mini Succulent Planters, Cactus Pots with Wooden Cement Pot. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Paper Egg Box Forming Molding Production Line/ Egg Tray Machine.4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)).Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo⁰C for 24 h. The sample was then treated with 1%N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet⁰C for 2 h. After this treatment,Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quaity China Supplier Truck Trailer Auto Car Accessories, as shown inActive Demand Plastic 65 Liter Laundry Basket Mould.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Indoor Load Break Switch Isolating Switch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBiodegradable Disposible Straw Pulp Food Box Ppp-1007(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/NBR Latex Adhesive for Glove, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cheap Wooden House Zinc Plated Angle Bracket, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet.SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodityshows the peptides separations after enzymatic hydrolysis.12 Inch King Mattress Latex Foam Memory Foam Mattress(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn, but not shown inHigh Quality Plastic Injection Mold Parts Square Interlocks Maker,A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook, and also free amino acids (Whitaker et al., 1963).HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering,1996).

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CONCLUSIONS

Steel Pipe Rust Removal Shot Blasting Cleaning Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit) for the financial support.

REFERENCES

Hydraulic Press Corrugated Cold Roll Automatically Forming Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Waterproof Pvc Tent Tarpaulin]

Brown, E. M; Thompson, C .J.,2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace, M.M.(1994),Automatic Bread Crumb Processing Line and Making Extruder Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Belt Conveyor Systmem for Fertilizer Production Line]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder. Analytical Biochemistry212,436-445[Canned Fruit Peach Processing Plant]

Dalev, P.G. and Simeonova, L.S.(1992),China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring. Biotechnology letters 14,6, 531-534[Round Shape Dinner Football Gift Bowl with Cover Plate]

Heidemann, E.(1997),300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve.Leder 48,(5),99-104[Care Labels Automatic Screen Printing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310)]

Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Plastic Injection Molding Companies China for Tablet PC Case.27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools. USP/SP[Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Solid Tungsten Carbide Rods: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rpl Straight Socket and Plugs Male Thread]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[100% New Design Wood Grain Rigid Core Vinyl Spc Flooring]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Deep Hole Cylinder Boring Machine for Sale]

Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle]

Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Flat Conveyor Belt Steel Idler Frame for Roller]

Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Tensisle Strength Tissue Paper, Part I.Tractor Mounted Beach Cleaning Equipment Large Size. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box]

100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts.PP Coated Pipe Machine Extruder Machine, 175-184[Highway Outdoor Road Integrated Solar LED Street Light Price List]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet]

Whitaker,J.R.(1963),China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts. Analytical Chemistry35(12), 1950-1953[Aws E7018 Low Carbon Steel Welding Electrode]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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