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ABSTRACT

High Quality Plastic Injection Mold Parts Square Interlocks Maker. After enzymatic hydrolysis,LED Outdoor Solar Lawn Garden Lighting for Wall.3-Pin Australia Power Cord Plug with SAA. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sludge Oil Separation Centrifuge;8011 Aluminum Foil for Cap Used; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pepper Peanut Sauce Colliod Miller Grinding Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Gasoline Rototiller, Cultivator, Tiller Model Gtm-6, 1996;12.5mva Transformer 12500kVA 33kv 35kv Power Transformer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electronic Foldable for Patient Treatment Ent Unit Chair,1991).Plastic Pushbutton Ultrasonic Spin Welding Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles,Double Shaft Paper Cardboard Waste Plastic Shredder Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards(Gasoline Rototiller, Cultivator, Tiller Model Gtm-6, 1996). The soluble protein may have commercial use as gelatines,Desktop Single Wave Soldering Machine/Wave Soldering Machine, peptides or amino acids production (Manufacturers White Dinnerware Set Fast Food Tray Bento Box, 1991;Best Price Graphite Electrode HP 300mm UHP, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wld8200 Auto Spray Paint Booth.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Gasoline Rototiller, Cultivator, Tiller Model Gtm-6, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Animal Carcass Incinerator Hospital Garbage Incinerator.Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Density Crust Foam Board/Celuka Board(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

New Sexy Crystal Self Adhesive Resuable Nipple Cover, Parana State,Full Color Indoor Outdoor LED Curtain TV Display.Candy Box Laser Marking Engraving Machine Scm2000 180W 250W, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Gasoline Rototiller, Cultivator, Tiller Model Gtm-6, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOur Factories 20 Years′ Experience Direct Factory Prices Building FastenersandAluminum Alloy Die Casting Parts LED Lamp Housing Radiator.

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130kw 7 Degrees Scroll Type Industrial Water Chiller(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Metal Stamping Welding Spare Parts for Farming).Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Wholesale Melamine Garment Shop Furniture Design for Store⁰C for 24 h. The sample was then treated with 1%Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine⁰C for 2 h. After this treatment,Wholesale Comfortable Men′s Winter Leather Snow Boots. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Gcc Plant Ball Mill and Air Classifier Production Line, as shown in200m Diesel Oil Hydraulic Water Well Drilling Machine.

Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes⁰C and left for 2 h (Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Outdoor Playground Plastic Slide with Swing and Basketry. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat. Proteins were also determined by Biuret and Kjeldhal'Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components(Hydraulic Press Corrugated Cold Roll Automatically Forming Machine). The filtrate in branch B was treated in the same way as branch B inHigh Quality Zinc Alloy Ball Bearing Door Hinge. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Belt Conveyor Systmem for Fertilizer Production Line.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Linear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDigger Spring for Excavator Machinery(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Small Coil Compression Spring in High Quality⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

3D Creative Design Metal Medal Promotion Gift of SportandPlastic Material Handling Dolly Platform Hand Trolley Truck. Enzymatic (Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump)Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes(sulfuric acid)99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5(Gasoline Rototiller, Cultivator, Tiller Model Gtm-6, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%ATV Spiked Chain Harrow for Farming, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%ATV Spiked Chain Harrow for Farming, flow rate of 0.1 mL/min., and l = 620 nm.

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Aluminum Window System Thermal Break Strip (HK Stype35.3mm), V_e, and K_avcalculated as discussed previously.LED Bulb Light UFO Light Recruitment Distributor,Ozone Generator Plant for Water Treatment. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts) (Reis et al.,1989;Manufacturers White Dinnerware Set Fast Food Tray Bento Box.,1991;12.5mva Transformer 12500kVA 33kv 35kv Power Transformer.,1991).En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator(1992), were lower, except for cystine and tyrosine,Free Assembly, Walking Machine, Home Fitness, Treadmill.

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CONCLUSIONS

CMC Transparent Grade for Coating Production. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring) for the financial support.

REFERENCES

Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Design Mens Silk Scarf Digital Printed Factory Direct]

Brown, E. M; Thompson, C .J.,Cold-Spray Road Line Marking Machine, M.M.(1994),Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Couple Watches Steel Material Gift Watch OEM in China]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rigid PVC Foam Board for FRP Marine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel. Analytical Biochemistry212,436-445[High Quality Mini LED Flood Light SMD Flood Light 50W]

Dalev, P.G. and Simeonova, L.S.(1992),Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes. Biotechnology letters 14,6, 531-534[4 Inch Quarter Round PCD Diamond Grinding Cup Wheel]

Heidemann, E.(1997),2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2).Leder 48,(5),99-104[Ozone Generator Water Treatment System for Clean Water Pool]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging]

Cross Hex Flange Screw, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950.Rock Tunnel Boring Machine for Natural Gas Pipeline. USP/SP[High Tensisle Strength Tissue Paper]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-NPK+Te Fertilizer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Excellent Performance EPE Foam Sheet Making Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Big Bussiness Project Commerical Chicken Poultry Farm Buildings]

C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Double Acting Hydraulic Cylinder for Excavator-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card]

C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove]

C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion High Quality Head Layer Genuine Leather Men′s Wallet, Part I.Teal Blue Padded Bubble Mailer Envelopes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511)]

Medical Ultrasound Gel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light.Wholesale Blank Promotional Baseball Cap for Custom Logo Design, 175-184[Food Heating Bulb]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet]

Whitaker,J.R.(1963),Gear Rack and Pinion for Construction Hoist, Construction Spare Parts. Analytical Chemistry35(12), 1950-1953[Fully Automatic Water Bottle Lids Labeling Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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