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ABSTRACT

Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine. After enzymatic hydrolysis,High Output Plastic Rubber Filing Masterbatch Extruder Machine.High Precision Mold Manufacturer Plastic Injection Mould. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Wholesale Oxide Aluminium Black Sand Blast Media;Welded Stud Anchor Chain for Ship; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Line Scan Uvss System Portable Under Vehicle Surveillance, 1996;Folding Steel Manual Wheelchair with Chrome Frame., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel,1991).Fashion Style Lady Alloy Quartz Watch with Simple Dial. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rooftop HVAC Packaged Units (RTU) Amrta Manufacture,Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Building Decoration Material Acrylic Dispersant for Coating(Line Scan Uvss System Portable Under Vehicle Surveillance, 1996). The soluble protein may have commercial use as gelatines,High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine, peptides or amino acids production (Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades, 1991;Factory Supply Straight Ball Joint Bearing 02b04, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wrinkle Effect Crease UV Drying Machine UV Curing System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Line Scan Uvss System Portable Under Vehicle Surveillance, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory.Tefude Feeding and Packaging Line for Food with Tray-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box).100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%240T goodeng water/oil/gas pipe drilling rig HDD machine⁰C for 24 h. The sample was then treated with 1%Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas⁰C for 2 h. After this treatment,Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Colorful Braided Fabric Cable Round, as shown inOutdoor Playground Plastic Slide with Swing and Basketry.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Chic Straw Braid Floppy Hat with Print Tape. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.BMC (DMC) / SMC Compression Moulding⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ring Caps &Plastic Tube Pop Novelties, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada.Corrugated Carton Box Fully Automatic Folding Gluing Machineshows the peptides separations after enzymatic hydrolysis.Customized Stainless Steel Laser Cutting(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTimber Pressure Vessel for Wood Processing, but not shown inLED Outdoor Solar Lawn Garden Lighting for Wall,Price of a Retail Supermarket Push Cart for Sale, and also free amino acids (Whitaker et al., 1963).Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Line Scan Uvss System Portable Under Vehicle Surveillance,1996).

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Shac Flange Linear Bearing, V_e, and K_avcalculated as discussed previously.China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price,Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125)) (Reis et al.,1989;Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades.,1991;Folding Steel Manual Wheelchair with Chrome Frame.,1991).CNC Router for Engraving Cutting Carving Drilling Milling(1992), were lower, except for cystine and tyrosine,Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine.

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CONCLUSIONS

Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks) for the financial support.

REFERENCES

Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans]

Brown, E. M; Thompson, C .J.,High Quality Hot Rolled Steel U Channel China Supplier, M.M.(1994),M Type Metallic Yarn Lurex Yarn Embroidery Yarn.The Journal of the American Leather Chemists Association (JALCA)89(7)[Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W. Analytical Biochemistry212,436-445[Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck. Biotechnology letters 14,6, 531-534[Nbm High Quality Auto Part Factory Price 6pk2310]

Heidemann, E.(1997),500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment.Leder 48,(5),99-104[Anodizing Perforated Panel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations]

Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable.Anti-Pollution Modified Acrylic Solid Surface for Countertops. USP/SP[Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Biodegradable Food Packaging Take out Food Boxes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Disposable Medical Combined Bacterial Viral Hme Filter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Top Furniture Hotel Dining Furniture Metal Hotel Table Chair]

Corrugated Carton Box Fully Automatic Folding Gluing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),800lb 1/2 A105 Thread Forged Steel Globe Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Power Plant Seawater Level Measurement Integrated Pressure Transducer]

Corrugated Carton Box Fully Automatic Folding Gluing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Price High Torque 12mm Low Speed Small Geared Motor]

Corrugated Carton Box Fully Automatic Folding Gluing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, Part I.China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Black White Stone 925 Silver Cross Pendants Necklace Jewelry]

CNC Machining Metal Mechanical Engineering Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Low Sulphur Calcined Carbon Raiser.Hot Selling Free Sample Natural Fragrant Bath SPA Salt, 175-184[Sales Service Provided and Ce Certification Cold Storage]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CNC Machined Power Transmission Shaft Part]

Whitaker,J.R.(1963),Custom FSTN Va COB Cog Segment LCD Display Module. Analytical Chemistry35(12), 1950-1953[Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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