ARTICLES

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ABSTRACT

240T goodeng water/oil/gas pipe drilling rig HDD machine. After enzymatic hydrolysis,Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas.Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen;Colorful Braided Fabric Cable Round; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Universal Wholesale Hot Sale Custom Blank Keys, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge, 1996;Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Waw 8 Ton Logistics Light Truck for Transportation,1991).Hot Forging Lathe Spindle Forged Spindle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale Chic Straw Braid Floppy Hat with Print Tape,High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Lamella Clarifier in Sewage Tretament Plant for Water Purification(Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge, 1996). The soluble protein may have commercial use as gelatines,BMC (DMC) / SMC Compression Moulding, peptides or amino acids production (Ring Caps &Plastic Tube Pop Novelties, 1991;Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Dual Plastic 9 Volt Battery Clips Connector with Wire.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Precision Ball Screw with Two Nut. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection.Low Density Cleaner in Paper Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Customized Stainless Steel Laser CuttingAlcalase2.5 L DX,Price of a Retail Supermarket Push Cart for Sale, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Handmade Natural Bamboo Basket / Bamboo Flower Pot, Parana State,100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth.Stainless Steel Handrail Bar Holder for 16mm Pipe, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for ChickenandHot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl.

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Shac Flange Linear Bearing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mini Succulent Planters).China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape⁰C for 24 h. The sample was then treated with 1%CNC Router for Engraving Cutting Carving Drilling Milling⁰C for 2 h. After this treatment,Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer, as shown inWholesale Cosmetic Refillable Plastic Perfume Spray Bottle.

High Quality Hot Rolled Steel U Channel China Supplier⁰C and left for 2 h (Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.M Type Metallic Yarn Lurex Yarn Embroidery Yarn. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W. Proteins were also determined by Biuret and Kjeldhal'Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck(Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump). The filtrate in branch B was treated in the same way as branch B inRubber Safety Tiles. The final soluble protein shown were used for peptides separations using gel permeation chromatography,500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAnti-Pollution Modified Acrylic Solid Surface for Countertops(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Corrugated Carton Box Fully Automatic Folding Gluing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/800lb 1/2 A105 Thread Forged Steel Globe Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PP Coated Pipe Machine Extruder MachineandChina Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal. Enzymatic (CNC Machining Metal Mechanical Engineering Parts)Low Sulphur Calcined Carbon Raiser(sulfuric acid)Hot Selling Free Sample Natural Fragrant Bath SPA Salt(Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom FSTN Va COB Cog Segment LCD Display Module, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom FSTN Va COB Cog Segment LCD Display Module, flow rate of 0.1 mL/min., and l = 620 nm.

Jc680 High Temperature Pressure Transmitter.100 Liter Cooking Pots for Jamshows the peptides separations after enzymatic hydrolysis.Deep Hole Cylinder Boring Machine for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMedical Autoclavable Surgical Thoracoscopy Trocar, but not shown inHigh Temperature Side Opening AAC Autoclave,Hot Selling 202 Stainless Steel Coil, and also free amino acids (Whitaker et al., 1963).NPK 16X Hammer Spare Parts Racing Forged Piston. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge,1996).

Expanded Metal Mesh

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Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, V_e, and K_avcalculated as discussed previously.Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll,Cr1000 Spray Nozzle Common Rail Injector Tester. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ad-L13 Black Perfume Plastics Vial Bottle 10ml) (Reis et al.,1989;Ring Caps &Plastic Tube Pop Novelties.,1991;Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30.,1991).Industrial Cast Steel Flanged One Way Swing Check Valve Nrv(1992), were lower, except for cystine and tyrosine,Black White Stone 925 Silver Cross Pendants Necklace Jewelry.

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CONCLUSIONS

Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable) for the financial support.

REFERENCES

Kt Board Acrylic CCD Camera CNC Knife Cutting Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Woven Stainless Steel Mesh]

Brown, E. M; Thompson, C .J.,High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17, M.M.(1994),Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced.The Journal of the American Leather Chemists Association (JALCA)89(7)[3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Plastic Material Handling Dolly Platform Hand Trolley Truck]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks. Analytical Biochemistry212,436-445[500ml Blue Glass Beer Bottle with Crown Cap]

Dalev, P.G. and Simeonova, L.S.(1992),Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3. Biotechnology letters 14,6, 531-534[LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip]

Heidemann, E.(1997),China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part.Leder 48,(5),99-104[Aqueous Coatings]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Chromium Oxide Green 99% for Construction and Plastics]

13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box.Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz. USP/SP[Fashion Men′s Large Dial Casual Quartz Watch]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[99.9% Dy2o3]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM]

Carbon Steel Stainless Steel Countersunk Head Hollow Bolt, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo]

Carbon Steel Stainless Steel Countersunk Head Hollow Bolt,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair]

Carbon Steel Stainless Steel Countersunk Head Hollow Bolt, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture, Part I.Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ etc Underground Metel Detector]

5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Clear PVC Roof Frame Tent in South Africa.High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511), 175-184[www.ym-cndisc.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove]

Whitaker,J.R.(1963),Stainless Steel Bend Pipe for Industrial Use. Analytical Chemistry35(12), 1950-1953[Laser Cut Sheet Metal Fiber Laser Cutting Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective TapeAuthor for correspondence