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ABSTRACT

Sc LC FC St Fiber Optical Adaptor Coupler Adapter. After enzymatic hydrolysis,Ent Treatment Medical Chair with Foot Control.Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutte, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (HPMC Hydroxy Propyl Methyl Cellulose Additives, 1996;Beautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpaste., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit,1991).2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bags. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging,Large Diaper Bag Backpack, Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop(HPMC Hydroxy Propyl Methyl Cellulose Additives, 1996). The soluble protein may have commercial use as gelatines,Water Chiller Air Cooled for Cooling Beer Refrigerating, peptides or amino acids production (High Quality Cheap Price Disposable IV Infusion Set with Precise Filter, 1991;Q3210 Vibratory Polishing Shot Blasting Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Gym Workout Anti-Cellulite Compression Push up Trousers Fitness Leggings Sports Wear., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Big Dimension UHP Graphite Electrodes for Steel Making.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Design Pet Product, Climbing Willow Cat Scratcher Tree. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (HPMC Hydroxy Propyl Methyl Cellulose Additives, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine.Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Commercial Inflatable Dry Slide for Kids, Animal Theme Kids Toys Fun City Slide, Factory Price Inflatable Bouncing Slide(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Round Panoramic Lift, Glass Observation Passenger Elevator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes).Painting Road Marking Vehicles Machine for Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automotive Rubber Parts for Any Rubber Material⁰C for 24 h. The sample was then treated with 1%Die Casting Zinc Alloy Badge Use for Car⁰C for 2 h. After this treatment,Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CREE Chip 24W 28W 30W LED Downlight's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove, as shown inPackaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500).

Three Phase 35kv Current Transformer for Indoor⁰C and left for 2 h (High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Shipping Logistics to Stockholm. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CNC Lathe Main Parts with Good Quality. Proteins were also determined by Biuret and Kjeldhal'Good Price High Torque 12mm Low Speed Small Geared Motor(Customized Double Acting Hydraulic Cylinder for Excavator). The filtrate in branch B was treated in the same way as branch B incoreschina.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWaterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mashroom Head Nylon Anchor with Pozi Nail⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Waterjet 87K Intensifier Part Check Valve Body 020077-1, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Fully Automatic Water Bottle Lids Labeling Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery) for the financial support.

REFERENCES

2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Chicken]

Brown, E. M; Thompson, C .J.,New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera, M.M.(1994),Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Indoor Customized Electronic Lighted LED Neon Open Sign]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fused Silica Quartz Lump 5-50mm for Refractory Materials. Analytical Biochemistry212,436-445[Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine]

Dalev, P.G. and Simeonova, L.S.(1992),Cold Drawn D2 H13 Alloy Steel Turning Roll. Biotechnology letters 14,6, 531-534[Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs]

Heidemann, E.(1997),Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat.Leder 48,(5),99-104[Popcorn Wholesale Tin Cans]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Good Quality Snow White Pumpkin Seeds for Sale]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Colorful 100LED Copper String Light Outdoor LED: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Leaf Shape Pearl Jewelry Wedding Tiara]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Casting Forging Train Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards]

H365 Emas Chainsaw Spares Cylinder Kits, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PP Woven Bag for Feed, Rice, Corn Feed Bag Rice Bag 10, 25, 50 Kg-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cold Drawn D2 H13 Alloy Steel Turning Roll]

H365 Emas Chainsaw Spares Cylinder Kits,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Metal Polishing Disc]

H365 Emas Chainsaw Spares Cylinder Kits, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:A4 Diploma Covers Wholesale with Silver Foil, Part I.OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Container House for Clinic or Hospital]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[20W Solar Street Light with Lens Motion Sensor 2 Years Warranty]

Whitaker,J.R.(1963),High-Tower Compound NPK Fertilizer 15-15-15. Analytical Chemistry35(12), 1950-1953[Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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