ARTICLES

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ABSTRACT

350mm Diamond Blade for Granite. After enzymatic hydrolysis,Custom Welding Stainless Steel Sheet Metal Welding Parts.Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production ((JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine, 1996;Ss316 Stainless Steel Heavy Duty Straps., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus,1991).High Temperature Side Opening AAC Autoclave, AAC Block Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap,Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal((JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine, 1996). The soluble protein may have commercial use as gelatines,1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine, peptides or amino acids production (New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar, 1991;Green Color PVC Anti Dazzle Panel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.XL-21 Motive Electrical Switchgear Power Cabinet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination ((JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Furniture Grade E1 Glue Plywood with Competitive Price.High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:TUV Certificate High Quality Single Core PV1-F PV Solar Cables(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hot Sale 840 Type PPGI Color Steel Roofing Sheets, Parana State,Junior Backyard Training Practise Soccer Goal Net.FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking ((JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theViscose and Polyester Blends Spunlace Nonwoven Fabric For Home WipesandPackaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500).

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CATV Indoor Optical Receiver FTTH(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Double Acting Hydraulic Cylinder for Excavator).Best Sell 16L Square Dustbin Frame Mould(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover⁰C for 24 h. The sample was then treated with 1%2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush⁰C for 2 h. After this treatment,Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PVDF Exterior Design Curved Aluminum Panel's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Neodymium Magnet with RoHS ISO 16499 Approved, as shown inProfessional Manufacturer Stainless Steel Floor Grating.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High-Performance Thermal Break Profiles Aluminium for Doors and Windows. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDesign Luxury Moon Cake Box with Hot Stamp Logo(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Logo Outdoor Hanging Swinging Camping Hammock, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products) for the financial support.

REFERENCES

12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Efficiency Waste Paper Egg Tray Making Machine]

Brown, E. M; Thompson, C .J.,500ml Blue Glass Beer Bottle with Crown Cap, M.M.(1994),White Iron Wear Crusher Blades 63HRC.The Journal of the American Leather Chemists Association (JALCA)89(7)[Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Good Quality Snow White Pumpkin Seeds for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System. Analytical Biochemistry212,436-445[8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight]

Dalev, P.G. and Simeonova, L.S.(1992),Stone Carving Flower Pot for Garden Decoration. Biotechnology letters 14,6, 531-534[Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt]

Heidemann, E.(1997),2018 New Fashion Men Canvas Messenger Bag.Leder 48,(5),99-104[NEMA 17 High Torque Hybrid Stepper Motor with Gearbox]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Leaf Shape Pearl Jewelry Wedding Tiara]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cold Drawn D2 H13 Alloy Steel Turning Roll]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-IP65 Outdoor Solar Flood Lamp with Battery for Commercial: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Metal Polishing Disc]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[gjmach-chn.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Container House for Clinic or Hospital]

Carbide Rod H6 Tc Rods, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Mens Slim Wallet Custom Design Leather Wallet Men-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[20W Solar Street Light with Lens Motion Sensor 2 Years Warranty]

Carbide Rod H6 Tc Rods,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry]

Carbide Rod H6 Tc Rods, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:St Fiber Optic Adaptor, Part I.Customized Cute Animal Dinosaur Kids Baby Baseball Cap. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve]

Latest Fashion Best-Selling Marble Slab Cutting Saw Blades, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording.Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover, 175-184[Best Price Factory Plain or Melamine Particle Board]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Competitive Price Sodium Gluconate as Concrete Admixture]

Whitaker,J.R.(1963),Motorcycle Tube Making Machine/Inner Tube Making Machine. Analytical Chemistry35(12), 1950-1953[Cr1000 Spray Nozzle Common Rail Injector Tester]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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