ARTICLES

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^IICNPq/RHAE/biotechnology-Flange Type Ball Float Water Tank Control Valve. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring

ABSTRACT

Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear. After enzymatic hydrolysis,Customized Outdoor Plastic Chrome Auto Logo Signs.Small Table CNC Marble Engraver for Stone. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4;China Soundproof Construction Foam PVC/WPC Board; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Pressure Braided Rubber Oil Hose, 1996;High Quality Beech Wood Chiavari Chair at a Competitive., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Gradient Print Overlay Push Button Membrane Keypad Switch,1991).Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004),Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk(High Pressure Braided Rubber Oil Hose, 1996). The soluble protein may have commercial use as gelatines,AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle, peptides or amino acids production (Low Power Consumption Silicone Sex Doll Vending Machine, 1991;Toughened Glass Disc Suspension Insulator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customized Round Airless Lotion Pump Bottles for Skin Care Products. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Pressure Braided Rubber Oil Hose, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Round Shape Dinner Football Gift Bowl with Cover Plate.Professional Exporter of Wheat Flour Mill Plant (80t)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer MachineAlcalase2.5 L DX,Aluminum Alloy Rack for Conductor, Wire, Cable, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Outdoor Accessories Fishing Hunting Masks); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

KERONG Security Safe Combination Smart Key Card Cabinet Door Lock, Parana State,925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring.Cast Floating Ball Valve China Manufacturer (GAQ41F), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Pressure Braided Rubber Oil Hose, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Manufacturer of Venlo PC or Venlo Glass Greenhouseand Corn Feed Bag Rice Bag 10.

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PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine).Stainless Steel Main Pipe Tee Joint for Railway Piping System(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container⁰C for 24 h. The sample was then treated with 1%Wholesale Promotion Snack Children School Lunch Bag Cooler Bag⁰C for 2 h. After this treatment,Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Full Automatic Paper Lunch Food Box Carton Forming Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Single Core Solid Copper Conductor Rg Coaxial Cable, as shown inPepper Peanut Sauce Colliod Miller Grinding Machine.

Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use⁰C and left for 2 h (China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Baby Clothes Wear Fleece Baby Bunting Onesie Jacket. Proteins were also determined by Biuret and Kjeldhal'Life Size 170cm Silicone Sex Dolls Real Love Doll for Male(Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1). The filtrate in branch B was treated in the same way as branch B inOceanstar Natural Seaweed Extract Flake Fertilizer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Reflective Sheeting Material for Licence Plate.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Industrial Grade Nitrous Oxide for Hot Sales. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLatest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fire Alarm Detector 24VDC Reflective Beam Detector⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Mini Bag Spices Small Salt Packing Packaging Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat PumpandNo 55 Steel Wire Cross Type Razor Barbed Wire. Enzymatic (4V330-10 Air Compressor Solenoid Industrial Valve)Professional Various Standard Hot Sale Rotary Lab Single Round Punch(sulfuric acid)Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute(High Pressure Braided Rubber Oil Hose, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Light Blue Color Glass Drink Bottle for Beer, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Light Blue Color Glass Drink Bottle for Beer, flow rate of 0.1 mL/min., and l = 620 nm.

Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box.Painting Road Marking Vehicles Machine for Saleshows the peptides separations after enzymatic hydrolysis.IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Wire, but not shown inPrecision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts,Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, and also free amino acids (Whitaker et al., 1963).Waterproof PVDF Aluminum Curtain Wall Panel. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Pressure Braided Rubber Oil Hose,1996).

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Sany Hg3333m Climbing Placing Boom, V_e, and K_avcalculated as discussed previously.Plastic Packaging Tray Vacuum Forming Machine,Permanent Suspended Magnetic Separator for Belt Conveyor. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ceramic Bearing Si3n4 608) (Reis et al.,1989;Low Power Consumption Silicone Sex Doll Vending Machine.,1991;High Quality Beech Wood Chiavari Chair at a Competitive.,1991).Double Sides Clear White Frosted Acrylic Sheet(1992), were lower, except for cystine and tyrosine,5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery.

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CONCLUSIONS

20W Solar Street Light with Lens Motion Sensor 2 Years Warranty. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Outdoor Accessories Fishing Hunting Masks) for the financial support.

REFERENCES

CNC Machined Power Transmission Shaft Part, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[HD PTZ Laser IR Night Vision Camera De Surveillance]

Brown, E. M; Thompson, C .J.,Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell, M.M.(1994),Good Price Channel Steel Used for Machinery.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[350mm Diamond Blade for Granite]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator. Analytical Biochemistry212,436-445[Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck]

Dalev, P.G. and Simeonova, L.S.(1992),Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology. Biotechnology letters 14,6, 531-534[EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings]

Heidemann, E.(1997),Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount.Leder 48,(5),99-104[A4 Inkjet Printable Pvc Plastic Sheet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price]

15L Big Cooling Cylinder High Production Hard Ice Cream Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Direct Sale Patent up to Down Rubber Tile Curing Press.Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China. USP/SP[Hot Sale Chic Straw Braid Floppy Hat with Print Tape]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[NBR Latex Adhesive for Glove]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Real Leather Wallet Mens Designer Leather Wallets: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM/ODM Factory New Item Competitive Offer Brass Lockable Ball Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Rht-060ws Soda Process Water Cooled Screw Type Water Chiller]

Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Full Port Small Automatic Electronic Plumbing Ball Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Electric Piston Pump Coating Machine PT6900]

Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)]

Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,, Part I.Cleanroom White PVC Dotted Anti-Static Gloves. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace]

High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Marine Solas Adhesive Reflective Tape.G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room, 175-184[15/28gn Steel Crawler Shot Blast Cleaning Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1200mm Recessed Mounted LED Linear Light]

Whitaker,J.R.(1963),Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers. Analytical Chemistry35(12), 1950-1953[Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Quality Crusher for Used Plastic Recycling LineAuthor for correspondence