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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Professional Exporter of Wheat Flour Mill Plant (80t). (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube

ABSTRACT

Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine. After enzymatic hydrolysis,Aluminum Alloy Rack for Conductor, Wire, Cable.Outdoor Accessories Fishing Hunting Masks. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cast Floating Ball Valve China Manufacturer (GAQ41F), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda, 1996;Stainless Steel Main Pipe Tee Joint for Railway Piping System., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container,1991).Wholesale Promotion Snack Children School Lunch Bag Cooler Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b,Full Automatic Paper Lunch Food Box Carton Forming Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Single Core Solid Copper Conductor Rg Coaxial Cable(PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda, 1996). The soluble protein may have commercial use as gelatines,Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use, peptides or amino acids production (Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building, 1991;Baby Clothes Wear Fleece Baby Bunting Onesie Jacket, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Life Size 170cm Silicone Sex Dolls Real Love Doll for Male., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Reflective Sheeting Material for Licence Plate.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Industrial Grade Nitrous Oxide for Hot Sales. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove.Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fire Alarm Detector 24VDC Reflective Beam Detector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Professional Various Standard Hot Sale Rotary Lab Single Round Punch, Parana State,ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube.Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz GuitarandPaper Automaitc Carton Box Cardboard Strapping Machine.

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Light Blue Color Glass Drink Bottle for Beer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve).Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor⁰C for 24 h. The sample was then treated with 1%Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine⁰C for 2 h. After this treatment,Waterproof PVDF Aluminum Curtain Wall Panel. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Round Modern Furniture Leisure Sofa for Promotion, as shown in16A/24A High Speed Wire Cable Braiding Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av20W Solar Street Light with Lens Motion Sensor 2 Years Warranty(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Good Price Channel Steel Used for Machinery, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (No 55 Steel Wire Cross Type Razor Barbed Wire) for the financial support.

REFERENCES

Rotor High Output Electric 120 Watt Motor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire]

Brown, E. M; Thompson, C .J.,Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby, M.M.(1994),Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel DIN7981 Pan Head Self Tapping Screw]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine. Analytical Biochemistry212,436-445[New Arrival Plush Animal Ride for Kids for Shopping Center]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile. Biotechnology letters 14,6, 531-534[No 55 Steel Wire Cross Type Razor Barbed Wire]

Heidemann, E.(1997),Rpl Straight Socket and Plugs Male Thread.Leder 48,(5),99-104[Colorful Comfortable Baby Chair Manufacturer Student Plastic Chair]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Indoor Load Break Switch Isolating Switch]

Round Shape Dinner Football Gift Bowl with Cover Plate, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall.OEM Metal Stamping Stainless Steel Parts with CNC Machining. USP/SP[STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ss Wafer Type Check Valve Dn32 Dn40 Dn50: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[EPS PS Foam Food Container Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.oytmachinery.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Theme Park Inflatable Bouncer for Sale (BJ-B16)]

2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automotive Acrylic Wholesale Masking Tape Factory Price]

2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting]

2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera, Part I.WPC Wood Plastic Board Equipment Extruder Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[120ml Glass Perfume Bottles for Designer Perfume]

Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Neoprene Elbow Support.Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules, 175-184[Elastic Back Support Maternity Belt]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment]

Whitaker,J.R.(1963),CNC Produce Medical Machining Custom Metal Milling. Analytical Chemistry35(12), 1950-1953[China Quality Heavy Duty Globe Control Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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