ARTICLES

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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV, E-Cars with Grade a, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China, 1996;Sc LC FC St Fiber Optical Adaptor Coupler Adapter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ent Treatment Medical Chair with Foot Control,1991).Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Leaf Shape Pearl Jewelry Wedding Tiara,Starter Motor 0001367034 12V 3KW 9T for Mercedes,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutte(6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China, 1996). The soluble protein may have commercial use as gelatines,HPMC Hydroxy Propyl Methyl Cellulose Additives, peptides or amino acids production (Beautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpaste, 1991;Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bags., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Large Diaper Bag Backpack, Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop.Water Chiller Air Cooled for Cooling Beer Refrigerating-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Cheap Price Disposable IV Infusion Set with Precise Filter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Round Panoramic Lift, Glass Observation Passenger Elevator⁰C and left for 2 h (Universal Mirco Forklift Car Bus Truck Seats Switch Sensors). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Painting Road Marking Vehicles Machine for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automotive Rubber Parts for Any Rubber Material. Proteins were also determined by Biuret and Kjeldhal'Die Casting Zinc Alloy Badge Use for Car(China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring). The filtrate in branch B was treated in the same way as branch B inUnique Design Hot Sale Impact Resistant Retro Reflective Material Tape. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CREE Chip 24W 28W 30W LED Downlight. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStone PVC Luxury Vinyl Plank Spc Flooring with V Groove(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Shipping Logistics to Stockholm⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CNC Lathe Main Parts with Good Quality, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Good Price High Torque 12mm Low Speed Small Geared Motor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool.New Design Pet Productshows the peptides separations after enzymatic hydrolysis.Waterjet 87K Intensifier Part Check Valve Body 020077-1(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17, but not shown in120L 240L Oil Universal Chemical Spil Absorbent Kits,Meat Machinery/Chicken Breast and Fish Slicing Machine, and also free amino acids (Whitaker et al., 1963).Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China,1996).

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CONCLUSIONS

High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Big Dimension UHP Graphite Electrodes for Steel Making) for the financial support.

REFERENCES

STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[9003073001 Transmission Rear Oil Seal Sinotruk Spare Parts]

Brown, E. M; Thompson, C .J.,100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine, M.M.(1994),New Styles Fashion Erotic Women Underwear Sexy Lingerie.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary. Analytical Biochemistry212,436-445[Artnet Node Artnet to DMX Controller]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping. Biotechnology letters 14,6, 531-534[Hot Selling Custom Sheet Metal Fabrication]

Heidemann, E.(1997),200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm.Leder 48,(5),99-104[Tilting 45 Degree Bridge Saw Cutting Machine for Granite Marble Tiles PLC700]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Small Boat Deck Knuckle Boom Ship Pedestal Crane]

High Quality Melamine Paper Manufacturer for Decoration, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fully Automatic Water Bottle Lids Labeling Machine.2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels. USP/SP[Double Sides Clear White Frosted Acrylic Sheet]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wrinkle Effect Crease UV Drying Machine UV Curing System]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Colorful Programmable Led Sign]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Rooftop HVAC Packaged Units (RTU) Amrta Manufacture]

Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fused Silica Quartz Lump 5-50mm for Refractory Materials-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Roller Skating Sneakers Shoes for Children (8083)]

Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Junior Backyard Training Practise Soccer Goal Net]

Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cold Drawn D2 H13 Alloy Steel Turning Roll, Part I.Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar]

Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved.Colorful 100LED Copper String Light Outdoor LED, 175-184[Laser Segmented Diamond Saw Blade for Stone Cutting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Paper Automaitc Carton Box Cardboard Strapping Machine]

Whitaker,J.R.(1963),H365 Emas Chainsaw Spares Cylinder Kits. Analytical Chemistry35(12), 1950-1953[Cast Steel Dual Plate Wafer Check Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check ValveAuthor for correspondence