ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Full Color Indoor Outdoor LED Curtain TV Display. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Quality Plastic Injection Mold Parts Square Interlocks Maker

ABSTRACT

LED Outdoor Solar Lawn Garden Lighting for Wall. After enzymatic hydrolysis,3-Pin Australia Power Cord Plug with SAA.Sludge Oil Separation Centrifuge. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;8011 Aluminum Foil for Cap Used;Pepper Peanut Sauce Colliod Miller Grinding Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Gasoline Rototiller, Cultivator, Tiller Model Gtm-6, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (12.5mva Transformer 12500kVA 33kv 35kv Power Transformer, 1996;Electronic Foldable for Patient Treatment Ent Unit Chair., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Pushbutton Ultrasonic Spin Welding Machine,1991).500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Double Shaft Paper Cardboard Waste Plastic Shredder Machine,Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Desktop Single Wave Soldering Machine/Wave Soldering Machine(12.5mva Transformer 12500kVA 33kv 35kv Power Transformer, 1996). The soluble protein may have commercial use as gelatines,Manufacturers White Dinnerware Set Fast Food Tray Bento Box, peptides or amino acids production (Best Price Graphite Electrode HP 300mm UHP, 1991;Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wld8200 Auto Spray Paint Booth., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Animal Carcass Incinerator Hospital Garbage Incinerator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (12.5mva Transformer 12500kVA 33kv 35kv Power Transformer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material.High Density Crust Foam Board/Celuka Board-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mango Pulp Juice Processing Production Line(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sc LC FC St Fiber Optical Adaptor Coupler Adapter).Factory Wholesale Melamine Garment Shop Furniture Design for Store(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine⁰C for 24 h. The sample was then treated with 1%Wholesale Comfortable Men′s Winter Leather Snow Boots⁰C for 2 h. After this treatment,Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Gcc Plant Ball Mill and Air Classifier Production Line's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes, as shown inBlack Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Digger Spring for Excavator Machinery. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av1.8ml Clear Sample Vials with Label and PP Caps(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic Material Handling Dolly Platform Hand Trolley Truck, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody.Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industryshows the peptides separations after enzymatic hydrolysis.Fashionable Natural Bamboo Wood Sunglasses for Men(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inKERONG Security Safe Combination Smart Key Card Cabinet Door Lock, but not shown in5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery,Solar Powered Outdoor Lighting LED Solar Wall Light, and also free amino acids (Whitaker et al., 1963).Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (12.5mva Transformer 12500kVA 33kv 35kv Power Transformer,1996).

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LED Bulb Light UFO Light Recruitment Distributor, V_e, and K_avcalculated as discussed previously.Ozone Generator Plant for Water Treatment,En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stainless Steel Tactile Paving Blind Road Stud) (Reis et al.,1989;Best Price Graphite Electrode HP 300mm UHP.,1991;Electronic Foldable for Patient Treatment Ent Unit Chair.,1991).Free Assembly, Walking Machine, Home Fitness, Treadmill(1992), were lower, except for cystine and tyrosine,CMC Transparent Grade for Coating Production.

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CONCLUSIONS

Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert) for the financial support.

REFERENCES

Cold-Spray Road Line Marking Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Car Accessories Interior]

Brown, E. M; Thompson, C .J.,Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner, M.M.(1994),Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Home Fitness]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Industrial Grade Nitrous Oxide for Hot Sales]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes. Analytical Biochemistry212,436-445[Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall]

Dalev, P.G. and Simeonova, L.S.(1992),2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2). Biotechnology letters 14,6, 531-534[Tractor Mounted Beach Cleaning Equipment Large Size]

Heidemann, E.(1997),Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950.Leder 48,(5),99-104[Wholesale Chinese OEM CNC Automotive Accessories Car Auto Spares Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Practical Edible Silicone Kitchenware (YB-ty-29)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Shac Flange Linear Bearing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[I12 Pop up Window Mix Color Touch Earphone]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap]

Customized Double Acting Hydraulic Cylinder for Excavator, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashion High Quality Head Layer Genuine Leather Men′s Wallet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge]

Customized Double Acting Hydraulic Cylinder for Excavator,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp]

Customized Double Acting Hydraulic Cylinder for Excavator, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Teal Blue Padded Bubble Mailer Envelopes, Part I.Medical Ultrasound Gel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth]

Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Blank Promotional Baseball Cap for Custom Logo Design.Gear Rack and Pinion for Construction Hoist, Construction Spare Parts, 175-184[Water Ozone Purifier for Vegetable Fruit Ozone Generator]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Low Volume Hard Tool Kit Thermoplastic Polymer Injection Molding]

Whitaker,J.R.(1963),Power Plant Seawater Level Measurement Integrated Pressure Transducer. Analytical Chemistry35(12), 1950-1953[Round Panoramic Lift]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3Author for correspondence