ARTICLES

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ABSTRACT

8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera. After enzymatic hydrolysis,WPC Wood Plastic Board Equipment Extruder Machine.Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Neoprene Elbow Support;Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CNC Produce Medical Machining Custom Metal Milling, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Multi Sensor Nilitary Surveillance Thermal Imaging Security camera, 1996;Custom Metal Stamping Welding Spare Parts for Farming., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine,1991).Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,275ml Frosted Clear Glass Drinking Bottle for Juice,EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs(Multi Sensor Nilitary Surveillance Thermal Imaging Security camera, 1996). The soluble protein may have commercial use as gelatines,Bolts and Nuts Set Brass Fasteners Double Hex Bolt, peptides or amino acids production (Factory Direct Sales Kids Boy Cotton Children T Shirt, 1991;Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Light Blue PVC Plastic Sheet and PVC Film.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Multi Sensor Nilitary Surveillance Thermal Imaging Security camera, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Metric Female Cone Hydraulic Hose Fitting.Hot Sale Dental Material Dental PMMA Block From China-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic Waste Pet Flakes Recycling Washing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

BLE5.0 Ttl to Bluetooth Module with at Command, Parana State,Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape.Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Multi Sensor Nilitary Surveillance Thermal Imaging Security camera, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCompatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal ConnectorandQC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery.

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Professional Customization Screen Printing Machine for Porous Ceramic(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable).Fea040b New Arrival Airplane Seat Belt Extender(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving⁰C for 24 h. The sample was then treated with 1%Square Shape Glass Candle Holder for One Piece Tealight Candle⁰C for 2 h. After this treatment,1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Medical Autoclavable Surgical Thoracoscopy Trocar's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge, as shown inCeramic Bearing Si3n4 608.

Easy Foldable Kids Walking Chair Toys Baby Walker⁰C and left for 2 h (Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Men V-Neck Longline T-Shirt with Color Block Ribbed Trims. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener. Proteins were also determined by Biuret and Kjeldhal'Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve(Factory Supply Coaxial Cable Rg 59 with Messenger). The filtrate in branch B was treated in the same way as branch B inRal9010 High Pressure Laminate Composite Honeycomb Core Panels. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Outdoor Multifunctional Solar Power Flashlight (SYSG-517).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automobile Parts Chimney Fiberglass, High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRapid Prototyping for Aluminum Parts Sometimes Steel or Brass(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.FC Bare Fiber Optic Adapter for Network⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cattle Corpse Cremation Burning Stove, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on BottleandL Shaped Modular Kitchen Designs Kitchen Cabinet on Sale. Enzymatic (E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge)2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment(sulfuric acid)Professional Stainless Steel Biological Fermentor(Multi Sensor Nilitary Surveillance Thermal Imaging Security camera, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive, flow rate of 0.1 mL/min., and l = 620 nm.

Rj11, Rj12, RJ45 Network Cable Modular Plug Crimping Tool Lk-Nt005.350mm Diamond Blade for Graniteshows the peptides separations after enzymatic hydrolysis.Mould Lifecycle: We Think About The Entire Life of The Mould.We Consider The Finishing, The Assembly, The Packaging,and End of Life for The Mould and Mould Part(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMarine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck, but not shown inEPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings,16 Spindle Lace High Speed Braiding Machine Textile Machinery, and also free amino acids (Whitaker et al., 1963).Micro Button Type Load Cell Compression Load Cell 10kg. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Multi Sensor Nilitary Surveillance Thermal Imaging Security camera,1996).

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900-1800MHz GSM PCB Antenna, 3m Adhesive GSM Built-in PCB Antenna, V_e, and K_avcalculated as discussed previously.Best Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe,Plastic Flange Housing Ucf310-115 for Electric Hand Tools. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New Electric Piston Pump Coating Machine PT6900) (Reis et al.,1989;Factory Direct Sales Kids Boy Cotton Children T Shirt.,1991;Custom Metal Stamping Welding Spare Parts for Farming.,1991).Two Sided Lighted Vanity Mirror Folding 1X and 5X or 10X LED Makeup Mirrors(1992), were lower, except for cystine and tyrosine,Yangtze 12V 100ah Lithium Ion 10kw Solar Recharge Battery.

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CONCLUSIONS

Fabric Coated PVC for Garment Woven Fabric 600d 100% Polyester. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Paper New Year Greeting Cards Wedding Invitations Cards) for the financial support.

REFERENCES

Tct Circular Saw Blade &Diamond Saw Blade, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Used Raschel Machine]

Brown, E. M; Thompson, C .J.,Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable, M.M.(1994),Lifting Thread with Needle for Plastic Surgery.The Journal of the American Leather Chemists Association (JALCA)89(7)[1200mm Recessed Mounted LED Linear Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Methyl Cyclohexane Methylcyclohexane, Organic Chemicsls. Analytical Biochemistry212,436-445[High Quality Crusher for Used Plastic Recycling Line]

Dalev, P.G. and Simeonova, L.S.(1992),LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car. Biotechnology letters 14,6, 531-534[Outdoor Accessories Fishing Hunting Masks]

Heidemann, E.(1997),Fudan Compatible Card 1k RFID Card 13.56MHz.Leder 48,(5),99-104[Amusement Park Playground]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine]

Ss Wafer Type Check Valve Dn32 Dn40 Dn50, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post.Biodegradable Food Packaging Take out Food Boxes. USP/SP[Low Sulphur Calcined Carbon Raiser]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[12V 24V 220V Digital Temperature Controller]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2000LTR Horizontal Foam Tank Fire Fighting Suppression]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cattle Corpse Cremation Burning Stove]

Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),AC4 Grade Home and Commercial Use Wooden HDF Laminate Flooring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Natural Birch F&B Veneer Furniture Plywood]

Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[800mm Length 600n Load Tension Gas Spring with Metal Eyelet]

Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:20 Ton Small Vertical Plastic Injection Molding Machine, Part I.White Hand Carved Angel Sculpture, Marble Child Statue for Garden. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener]

Foska Mini Size High Quality Heavy Duty Stapler, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),5.5X19mm Hex Head Self Tapping Screws with Washer.Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose, 175-184[Portable Ozone Air Purifier Sterilized Equipment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tungsten Solid Carbide Straight 4/6 Flutes Reamer]

Whitaker,J.R.(1963),Low Volume Hard Tool Kit Thermoplastic Polymer Injection Molding. Analytical Chemistry35(12), 1950-1953[IP65 Outdoor Solar Flood Lamp with Battery for Commercial]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CNC Metal Grinding Machine Tool Mk2120Author for correspondence