ARTICLES

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ABSTRACT

IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash. After enzymatic hydrolysis,Wholesales Stackable Coffee Iron Chair.OEM Eye Anti-Aging Serum. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Microfiber Towel Car Drying Soft Towel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH, 1996;High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food,1991).100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Styles Fashion Erotic Women Underwear Sexy Lingerie,High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping(Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH, 1996). The soluble protein may have commercial use as gelatines,200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm, peptides or amino acids production (Fully Automatic Water Bottle Lids Labeling Machine, 1991;2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fused Silica Quartz Lump 5-50mm for Refractory Materials. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cold Drawn D2 H13 Alloy Steel Turning Roll.Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (ASTM A240 304 316L No. 1 Stainless Steel Sheet).120L 240L Oil Universal Chemical Spil Absorbent Kits(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse⁰C for 24 h. The sample was then treated with 1%High-Tower Compound NPK Fertilizer 15-15-15⁰C for 2 h. After this treatment,20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hybrid Inverter Combined MPPT Controller 1-12kw, as shown inHot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry.

QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery⁰C and left for 2 h (China Round Transparent Glass 750ml Liquor Glass Bottle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Newest Men Watches Popular Products Mens Custom Wood Watch. Proteins were also determined by Biuret and Kjeldhal'Accurate Measuring Rulers 3D Lenticular Plastic Rulers(New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera). The filtrate in branch B was treated in the same way as branch B inwww.alitruck.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Reusable PLA and Pbat Tea Packing Pouch Supplier in China.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHousehold Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Purity99.95% Cobalt Oxide, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated.Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvasshows the peptides separations after enzymatic hydrolysis.6000 Series Anodizing Extrusion Aluminum Tube(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s, but not shown inMining Machinery Stone Crusher Bowl Liner Cone Crusher Parts,World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J), and also free amino acids (Whitaker et al., 1963).DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH,1996).

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CONCLUSIONS

Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (H365 Emas Chainsaw Spares Cylinder Kits) for the financial support.

REFERENCES

Ltmg Classic Product 6 Ton Wheel Front Loader for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Electronica Microscope]

Brown, E. M; Thompson, C .J.,Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror, M.M.(1994),Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar.The Journal of the American Leather Chemists Association (JALCA)89(7)[Lamella Clarifier in Industrial Wastewater Treatment]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Powder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Samsung LTI550HN16 1920X1080P monitor screen LCD video wall. Analytical Biochemistry212,436-445[Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts. Biotechnology letters 14,6, 531-534[Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel]

Heidemann, E.(1997),Household Family Medical Emergency Electronic Digital Thermometer.Leder 48,(5),99-104[m.yesonmesh.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Water Chiller Air Cooled for Cooling Beer Refrigerating]

Meat Machinery/Chicken Breast and Fish Slicing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Die Cast Iron Worm Gear Speed Reduce Gearbox.19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting. USP/SP[Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Couple Watches Steel Material Gift Watch OEM in China: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Zs Round Vibrating Sieve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hair Band Sport]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby]

Glass Tea Sugar Coffee Storage Jar with Screw Cap, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Circular DC Electro Lifting Magnet for Lifting Scraps-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ 20t]

Glass Tea Sugar Coffee Storage Jar with Screw Cap,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop]

Glass Tea Sugar Coffee Storage Jar with Screw Cap, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts, Part I.Particle Board Manufacturer, Melamine/Plain Chipboard. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[360 Degree Maverick Angler Fishing Kayak with Rotating Seat]

CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G).Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert, 175-184[Hot Sale Pet Food Beverage Jar Automatic Bottle Making Blow Molding Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box]

Whitaker,J.R.(1963),Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps. Analytical Chemistry35(12), 1950-1953[Outdoor Playground Plastic Slide with Swing and Basketry]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Big Dimension UHP Graphite Electrodes for Steel MakingAuthor for correspondence