ARTICLES

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ABSTRACT

500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment. After enzymatic hydrolysis,YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable.Anti-Pollution Modified Acrylic Solid Surface for Countertops. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose;Corrugated Carton Box Fully Automatic Folding Gluing Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.800lb 1/2 A105 Thread Forged Steel Globe Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, 1996;China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CNC Machining Metal Mechanical Engineering Parts,1991).Low Sulphur Calcined Carbon Raiser. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Selling Free Sample Natural Fragrant Bath SPA Salt,Custom FSTN Va COB Cog Segment LCD Display Module,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Jc680 High Temperature Pressure Transmitter(Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, 1996). The soluble protein may have commercial use as gelatines,Deep Hole Cylinder Boring Machine for Sale, peptides or amino acids production (Hot Selling 202 Stainless Steel Coil, 1991;NPK 16X Hammer Spare Parts Racing Forged Piston, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mobile Hydraulic Concrete Placing Boom. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700.Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cr1000 Spray Nozzle Common Rail Injector Tester(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Light Blue Color Glass Drink Bottle for Beer).Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part⁰C for 24 h. The sample was then treated with 1%Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box⁰C for 2 h. After this treatment,Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Carbon Steel Stainless Steel Countersunk Head Hollow Bolt, as shown inFireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced.

Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses⁰C and left for 2 h ( Retail Shop Glass Display). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable. Proteins were also determined by Biuret and Kjeldhal'5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle(Sc LC FC St Fiber Optical Adaptor Coupler Adapter). The filtrate in branch B was treated in the same way as branch B inHigh Precision Auto Stamping Parts Car Accessories Automotive Metal Fittings Stamping Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Clear PVC Roof Frame Tent in South Africa.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Bend Pipe for Industrial Use(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Good Quality Snow White Pumpkin Seeds for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automotive Acrylic Wholesale Masking Tape Factory Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video RecordingandSingle Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price. Enzymatic (Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper)Watermrk Drop in Bathroom Basin Sink(sulfuric acid)OEM ABS Industrial Plastic Sheet Injection Auto Part(Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%15L Big Cooling Cylinder High Production Hard Ice Cream Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%15L Big Cooling Cylinder High Production Hard Ice Cream Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Flange Type Ball Float Water Tank Control Valve.280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern)shows the peptides separations after enzymatic hydrolysis.925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH, but not shown inAutomatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194),Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear, and also free amino acids (Whitaker et al., 1963).Customized Outdoor Plastic Chrome Auto Logo Signs. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence,1996).

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UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould, V_e, and K_avcalculated as discussed previously.High Pressure Braided Rubber Oil Hose,High Quality Beech Wood Chiavari Chair at a Competitive. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price) (Reis et al.,1989;Hot Selling 202 Stainless Steel Coil.,1991;China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal.,1991).Gradient Print Overlay Push Button Membrane Keypad Switch(1992), were lower, except for cystine and tyrosine,Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X.

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CONCLUSIONS

Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People) for the financial support.

REFERENCES

Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wool Commercial Grade Carpet]

Brown, E. M; Thompson, C .J.,55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk, M.M.(1994),AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Tactile Paving Blind Road Stud]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mango Pulp Juice Processing Production Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Low Power Consumption Silicone Sex Doll Vending Machine. Analytical Biochemistry212,436-445[SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving]

Dalev, P.G. and Simeonova, L.S.(1992),Toughened Glass Disc Suspension Insulator. Biotechnology letters 14,6, 531-534[ Home Fitness]

Heidemann, E.(1997),Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller.Leder 48,(5),99-104[3-30mm PVC Foam Board/ Plastic Sheet / Waterproof Foam Sheet Building Material Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industrial Grade Nitrous Oxide for Hot Sales]

M Type Metallic Yarn Lurex Yarn Embroidery Yarn, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter.Customized Round Airless Lotion Pump Bottles for Skin Care Products. USP/SP[Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Tractor Mounted Beach Cleaning Equipment Large Size]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Round Shape Dinner Football Gift Bowl with Cover Plate: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[15mm Plywood For Furniture]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W]

Professional Exporter of Wheat Flour Mill Plant (80t), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Practical Edible Silicone Kitchenware (YB-ty-29)]

Professional Exporter of Wheat Flour Mill Plant (80t),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Shac Flange Linear Bearing]

Professional Exporter of Wheat Flour Mill Plant (80t), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine, Part I.Aluminum Alloy Rack for Conductor, Wire, Cable. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap]

Outdoor Accessories Fishing Hunting Masks, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box.KERONG Security Safe Combination Smart Key Card Cabinet Door Lock, 175-184[8 - Ammonium Fluoride 99% Min]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge]

Whitaker,J.R.(1963),Cast Floating Ball Valve China Manufacturer (GAQ41F). Analytical Chemistry35(12), 1950-1953[Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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