ARTICLES

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ABSTRACT

New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10. After enzymatic hydrolysis,Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540).Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Rht-060ws Soda Process Water Cooled Screw Type Water Chiller;Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Boat Aluminum Pipe 5083 Marine Used Aluminum Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar, 1996;2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair,1991).Flanged Braided Metal Hose Connector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Timber Pressure Vessel for Wood Processing,1200mm Recessed Mounted LED Linear Light,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,The Big Diameter Carbide Tipped Hole Saw(Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar, 1996). The soluble protein may have commercial use as gelatines,Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo, peptides or amino acids production (Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale, 1991;Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fashion Men′s Large Dial Casual Quartz Watch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor Garden Furniture Baby Plastic Swing for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone.2D LED Rope Lighting Motif Lights for Christmas Decoration-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Modern Compact Laminate Key Lock Changing Room Locker(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Outdoor Full Color LED Video WallAlcalase2.5 L DX,180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125), with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Quick Connect Camlock and Grooved Couplings Quick Fitting Type D, Parana State,Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet.High Quality White Calcined Alumina Powder for Ceramic Industry, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardwareand304L Stainless Steel U Channel Good Performance.

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500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Delicate Handmade Round Natural Stone Chain Necklace).100kw Cummins Diesel Generator Set(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle⁰C for 24 h. The sample was then treated with 1%Luxury Hotel Furniture Antique Design Marble Side Table⁰C for 2 h. After this treatment,Ampoule Sealer Machine Hydrogen Oxygen Gas Generator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Commercial Double Lane Inflatable Carnival Bungee Run Game's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair, as shown inXL-21 Motive Electrical Switchgear Power Cabinet.

Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale⁰C and left for 2 h (12.5mva Transformer 12500kVA 33kv 35kv Power Transformer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.800mm Length 600n Load Tension Gas Spring with Metal Eyelet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003. Proteins were also determined by Biuret and Kjeldhal'5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers(Felt Disc Polishing Sanding Disc with Wool Felt Diam). The filtrate in branch B was treated in the same way as branch B inEdit Wi-Fi Wireless Car Vehicle DVR Recorder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Food Grade Customized Metal Candy Can Square Mint Tin Box⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/600X600 mm Ugr 19 LED Panel Lights, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Good Quality Hot Tube8 Japanese Stainless Steel Tube 666, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration LightandComputer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine. Enzymatic (Round Plastic Hanging Basket (KD7014))Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag(sulfuric acid)China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle(Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents, flow rate of 0.1 mL/min., and l = 620 nm.

New Design High Quality Alminum Sandwich Panel High Pressure Laminate.Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessertshows the peptides separations after enzymatic hydrolysis.Steel Setractable Rail Protection Cover(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStandard Industrial Double Nut Ball Screw Dfu Series, but not shown inModern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair,Natural Birch F&B Veneer Furniture Plywood, and also free amino acids (Whitaker et al., 1963).Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar,1996).

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Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel, V_e, and K_avcalculated as discussed previously.High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering,Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw) (Reis et al.,1989;Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale.,1991;2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards.,1991).Customized Metal Material Products for Punching Stamping Part(1992), were lower, except for cystine and tyrosine,Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System.

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CONCLUSIONS

25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners) for the financial support.

REFERENCES

Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[10cbm Vacuum Timber Dryer with Advanced High Frequency Technology]

Brown, E. M; Thompson, C .J.,Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place, M.M.(1994),Theme Park Inflatable Bouncer for Sale (BJ-B16).The Journal of the American Leather Chemists Association (JALCA)89(7)[5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Luxury Hotel Furniture Antique Design Marble Side Table]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Muliti-Size Light Hot Stamping Foil for PS Frame. Analytical Biochemistry212,436-445[BMC (DMC) / SMC Compression Moulding]

Dalev, P.G. and Simeonova, L.S.(1992),Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light. Biotechnology letters 14,6, 531-534[Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG)]

Heidemann, E.(1997),50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court.Leder 48,(5),99-104[Types Of Titanium Dioxide]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Pressure Braided Rubber Oil Hose]

Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China 1 & 100 RMB Acrylic Currence Frame.1 Ton Toyo Type Manual Chain Block. USP/SP[TUV Certificate High Quality Single Core PV1-F PV Solar Cables]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Unique Handle 24mm Water Bottle Caps]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Spark Resistant Construction Axial Fan with Fixed Pitch Blade: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cost For A Residential Lift]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Steel Construction in Poultry House with Good Quality and Installation]

Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick]

Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances]

Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25), Part I.Precision CNC Milling DIN69871. Mas403 for CNC Machinist. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Metal Animal Longhorn Skull Pendant Longhorn Skull Charm]

Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Building Material of Granite Ceramic Wall Tile for Wall Decoration.3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine, 175-184[Electronic Digital Mini Home Safe for Jewelry Cash]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality White Calcined Alumina Powder for Ceramic Industry]

Whitaker,J.R.(1963),Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box. Analytical Chemistry35(12), 1950-1953[15L Big Cooling Cylinder High Production Hard Ice Cream Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Am/RF Glasses Tag Anti-Theft Optical Tag for GlassesAuthor for correspondence