ARTICLES

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ABSTRACT

Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light. After enzymatic hydrolysis,Customized Metal Material Products for Punching Stamping Part.Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Theme Park Inflatable Bouncer for Sale (BJ-B16), 1996;Muliti-Size Light Hot Stamping Foil for PS Frame., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light,1991).50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China 1 & 100 RMB Acrylic Currence Frame,1 Ton Toyo Type Manual Chain Block,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Spark Resistant Construction Axial Fan with Fixed Pitch Blade(Theme Park Inflatable Bouncer for Sale (BJ-B16), 1996). The soluble protein may have commercial use as gelatines,Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl, peptides or amino acids production (3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home, 1991;25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Precision CNC Milling DIN69871. Mas403 for CNC Machinist., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Building Material of Granite Ceramic Wall Tile for Wall Decoration. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Theme Park Inflatable Bouncer for Sale (BJ-B16), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine.Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Good Price Thermoplastic Hot Melt Road Marking Paint Line Equipment).White Desiccant Bag Silica Gel with Indicated Orange Bead(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Iron Wire Mesh Chain Link Mesh Security Fence⁰C for 24 h. The sample was then treated with 1%TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump⁰C for 2 h. After this treatment,5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera, as shown inLamella Clarifier in Industrial Wastewater Treatment.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Electric Piston Pump Coating Machine PT6900(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Crusher for Used Plastic Recycling Line, pH 3.25., mobile phase B: buffer solution of 0.01 mol/300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Suspended False Ceiling Decorated by Aluminum Honeycomb Composite PanelandCeramic Bearing Si3n4 608. Enzymatic (Nbm High Quality Auto Part Factory Price 6pk2310)Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay(sulfuric acid)High Quality Mini LED Flood Light SMD Flood Light 50W(Theme Park Inflatable Bouncer for Sale (BJ-B16), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead, flow rate of 0.1 mL/min., and l = 620 nm.

Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass.Water Chiller Air Cooled for Cooling Beer Refrigeratingshows the peptides separations after enzymatic hydrolysis.Professional Manufacture Metal Medal Promotional Gift(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants, but not shown in2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace,Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving, and also free amino acids (Whitaker et al., 1963).Singlemode Su Upc Fiber Optic Fast Connector. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Theme Park Inflatable Bouncer for Sale (BJ-B16),1996).

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40X-1600X Biological Microscope Xsz-107V LED Light, V_e, and K_avcalculated as discussed previously.6063 Aluminum Coil for Commercial Vehicle Floor,Silicon Carbide Foundry Porous Ceramic Foam Filter. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box) (Reis et al.,1989;3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home.,1991;Muliti-Size Light Hot Stamping Foil for PS Frame.,1991).1200X1000X810 Strong Collapsible Plastic Containers for Storage(1992), were lower, except for cystine and tyrosine,Steel Construction in Poultry House with Good Quality and Installation.

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CONCLUSIONS

Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Metal Container Packaging for Edible Oil Tin Cans Storage Boxes) for the financial support.

REFERENCES

10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.vtimejewelry.com]

Brown, E. M; Thompson, C .J.,Stone Cracking Rock Cutting Cement, M.M.(1994),8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Promotion Snack Children School Lunch Bag Cooler Bag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Neodymium Magnet with RoHS ISO 16499 Approved]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards. Analytical Biochemistry212,436-445[Corrugated Carton Box Fully Automatic Folding Gluing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator. Biotechnology letters 14,6, 531-534[Timber Pressure Vessel for Wood Processing]

Heidemann, E.(1997),PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag.Leder 48,(5),99-104[Efficient Stainless Steel Rotary Drum Belt Filter Press]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[LED Outdoor Solar Lawn Garden Lighting for Wall]

GRP/FRP Profile Structures/Fiberglass Pultrusion Product, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Aluminum Hot Forging Accessories Machining Parts.Building Wall Machine for Hot Machine. USP/SP[Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Manufacturers White Dinnerware Set Fast Food Tray Bento Box]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Arrival Amazon Hot Style Printed Slip Dress for Women Summer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bend Nozzle Tube for Piston Cooling Cylinder Chamber]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net]

Metal Cuting CNC Plasma Cutting Machine (DW1325), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers]

Metal Cuting CNC Plasma Cutting Machine (DW1325),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125)]

Metal Cuting CNC Plasma Cutting Machine (DW1325), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Expanded Metal Infill Panel Metal Mesh Custom Made, Part I.DC Motor for Massagers. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Disposable Blood Transfusion Set with Filter]

High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry.Custom Good Quality Outdoor Music Wireless Cooler Speaker Box, 175-184[Solar Power Garden Decorative Lights]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom FSTN Va COB Cog Segment LCD Display Module]

Whitaker,J.R.(1963),OEM Viton Mechanical Rubber Oil Seal. Analytical Chemistry35(12), 1950-1953[Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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