ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor. After enzymatic hydrolysis,Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine.Waterproof PVDF Aluminum Curtain Wall Panel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas;Round Modern Furniture Leisure Sofa for Promotion; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sany Hg3333m Climbing Placing Boom, 1996;Plastic Packaging Tray Vacuum Forming Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Permanent Suspended Magnetic Separator for Belt Conveyor,1991).Double Sides Clear White Frosted Acrylic Sheet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery,20W Solar Street Light with Lens Motion Sensor 2 Years Warranty,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CNC Machined Power Transmission Shaft Part(Sany Hg3333m Climbing Placing Boom, 1996). The soluble protein may have commercial use as gelatines,Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell, peptides or amino acids production (Good Price Channel Steel Used for Machinery, 1991;Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Direct Sale Patent up to Down Rubber Tile Curing Press. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sany Hg3333m Climbing Placing Boom, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China.Real Leather Wallet Mens Designer Leather Wallets-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale).ASTM A240 304 316L No. 1 Stainless Steel Sheet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%12V 24V 220V Digital Temperature Controller⁰C for 24 h. The sample was then treated with 1%Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price⁰C for 2 h. After this treatment,25kg Fertilizer Belt Packing Machine for Special Materials. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rotor High Output Electric 120 Watt Motor, as shown inAutomotive Rubber Parts for Any Rubber Material.

Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby⁰C and left for 2 h (3-Pin Australia Power Cord Plug with SAA). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine. Proteins were also determined by Biuret and Kjeldhal'Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile(Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar). The filtrate in branch B was treated in the same way as branch B in15L Mini Compressor Refrigerator 12V Camping Fridge. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Rpl Straight Socket and Plugs Male Thread.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOEM Metal Stamping Stainless Steel Parts with CNC Machining(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape, pH 3.25., mobile phase B: buffer solution of 0.01 mol/8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hydraulic Electric Stationary Car Platform Scissor Elevator LiftandWPC Wood Plastic Board Equipment Extruder Machine. Enzymatic (Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS)Neoprene Elbow Support(sulfuric acid)Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules(Sany Hg3333m Climbing Placing Boom, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%CNC Produce Medical Machining Custom Metal Milling, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%CNC Produce Medical Machining Custom Metal Milling, flow rate of 0.1 mL/min., and l = 620 nm.

Multi Sensor Nilitary Surveillance Thermal Imaging Security camera.Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Lightshows the peptides separations after enzymatic hydrolysis.Custom Metal Stamping Welding Spare Parts for Farming(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew Type Automatic Multihead Weigher All-in-One Packing Machine for Rice, but not shown inFire Alarm Detector 24VDC Reflective Beam Detector,Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine, and also free amino acids (Whitaker et al., 1963).Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sany Hg3333m Climbing Placing Boom,1996).

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Bolts and Nuts Set Brass Fasteners Double Hex Bolt, V_e, and K_avcalculated as discussed previously.Factory Direct Sales Kids Boy Cotton Children T Shirt,Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Minimalist Slim Real Leather Front Pocket Card Wallet) (Reis et al.,1989;Good Price Channel Steel Used for Machinery.,1991;Plastic Packaging Tray Vacuum Forming Machine.,1991).15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi(1992), were lower, except for cystine and tyrosine,Light Blue PVC Plastic Sheet and PVC Film.

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CONCLUSIONS

Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cleanroom White PVC Dotted Anti-Static Gloves) for the financial support.

REFERENCES

Metric Female Cone Hydraulic Hose Fitting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Steel bridge construction welded steel iron H beamwith cheap price]

Brown, E. M; Thompson, C .J.,Hot Sale Dental Material Dental PMMA Block From China, M.M.(1994),Plastic Waste Pet Flakes Recycling Washing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sany Hg3333m Climbing Placing Boom]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Deposited Stainless Steel Rivet Nut with Knurled. Analytical Biochemistry212,436-445[Teal Blue Padded Bubble Mailer Envelopes]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Stainless Steel Mechanical Filter Hosing. Biotechnology letters 14,6, 531-534[Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge]

Heidemann, E.(1997),Paper New Year Greeting Cards Wedding Invitations Cards.Leder 48,(5),99-104[Vertical Wire and Cable Taping Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box]

Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01).BLE5.0 Ttl to Bluetooth Module with at Command. USP/SP[Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[AC Electric Vibration Table Concrete Vibration Motor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Metal Stamping Welding Spare Parts for Farming]

Professional Customization Screen Printing Machine for Porous Ceramic, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fea040b New Arrival Airplane Seat Belt Extender-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[200m Diesel Oil Hydraulic Water Well Drilling Machine]

Professional Customization Screen Printing Machine for Porous Ceramic,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China]

Professional Customization Screen Printing Machine for Porous Ceramic, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving, Part I.Square Shape Glass Candle Holder for One Piece Tealight Candle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hydraulic Press Corrugated Cold Roll Automatically Forming Machine]

1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Medical Autoclavable Surgical Thoracoscopy Trocar.DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge, 175-184[IP68 Water-Proof Adjustable 6-11mm Plastic Cable Glands Pg Type Nylon Cable Connector Jonits with Lock Nut and Flat Washer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3D Creative Design Metal Medal Promotion Gift of Sport]

Whitaker,J.R.(1963),Easy Foldable Kids Walking Chair Toys Baby Walker. Analytical Chemistry35(12), 1950-1953[Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Efficiency Stone Crusher Hammer Crusher Glass Crusher for SaleAuthor for correspondence