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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Precision Mold Manufacturer Plastic Injection Mould. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,China Wholesale Oxide Aluminium Black Sand Blast Media

ABSTRACT

Welded Stud Anchor Chain for Ship. After enzymatic hydrolysis,SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry.Line Scan Uvss System Portable Under Vehicle Surveillance. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Folding Steel Manual Wheelchair with Chrome Frame;Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fashion Style Lady Alloy Quartz Watch with Simple Dial, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, 1996;Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Building Decoration Material Acrylic Dispersant for Coating,1991).High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades,Factory Supply Straight Ball Joint Bearing 02b04,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wrinkle Effect Crease UV Drying Machine UV Curing System(Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, 1996). The soluble protein may have commercial use as gelatines,Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack, peptides or amino acids production (Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector, 1991;20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Tefude Feeding and Packaging Line for Food with Tray., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CMP No Nc Metal Switch Black 25mm Illuminated Push Button.Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

240T goodeng water/oil/gas pipe drilling rig HDD machine, Parana State,China Wholesale Oxide Aluminium Black Sand Blast Media.Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Bend Pipe for Industrial UseandSs Wafer Type Check Valve Dn32 Dn40 Dn50.

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Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5).Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Colorful Braided Fabric Cable Round⁰C for 24 h. The sample was then treated with 1%Universal Wholesale Hot Sale Custom Blank Keys⁰C for 2 h. After this treatment,Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Waw 8 Ton Logistics Light Truck for Transportation, as shown inDurable TPR Pet Molar Toy with Colorful Rope.

Hot Forging Lathe Spindle Forged Spindle⁰C and left for 2 h (12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale Chic Straw Braid Floppy Hat with Print Tape. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply. Proteins were also determined by Biuret and Kjeldhal'Lamella Clarifier in Sewage Tretament Plant for Water Purification(Agriculture Hay Silage Wrapping Film). The filtrate in branch B was treated in the same way as branch B inWiFi Controlled Wireless Enabled Smart Remote Residential Auto Recloser Circuit Breaker. The final soluble protein shown were used for peptides separations using gel permeation chromatography,BMC (DMC) / SMC Compression Moulding.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ring Caps &Plastic Tube Pop Novelties. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dual Plastic 9 Volt Battery Clips Connector with Wire⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Precision Ball Screw with Two Nut, pH 3.25., mobile phase B: buffer solution of 0.01 mol/EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4)andLow Density Cleaner in Paper Making Machine. Enzymatic (En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada)Customized Stainless Steel Laser Cutting(sulfuric acid)Price of a Retail Supermarket Push Cart for Sale(Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable, flow rate of 0.1 mL/min., and l = 620 nm.

OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate.High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machineshows the peptides separations after enzymatic hydrolysis.Handmade Natural Bamboo Basket / Bamboo Flower Pot(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMachinery Parts Aeroplane Engine CNC Milling, but not shown inFactory Direct Sale Patent up to Down Rubber Tile Curing Press,Stainless Steel Handrail Bar Holder for 16mm Pipe, and also free amino acids (Whitaker et al., 1963).Shac Flange Linear Bearing. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Rooftop HVAC Packaged Units (RTU) Amrta Manufacture,1996).

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CONCLUSIONS

Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable) for the financial support.

REFERENCES

Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Facial Tissue Embossing Slitting Machine Products Made in China]

Brown, E. M; Thompson, C .J.,500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment, M.M.(1994),YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Feeding Customized Multi Layers Shirts Cutting Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Anti-Pollution Modified Acrylic Solid Surface for Countertops. Analytical Biochemistry212,436-445[1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose. Biotechnology letters 14,6, 531-534[Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality]

Heidemann, E.(1997),Corrugated Carton Box Fully Automatic Folding Gluing Machine.Leder 48,(5),99-104[Pp Woven Pizza Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable]

High Output Plastic Rubber Filing Masterbatch Extruder Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),800lb 1/2 A105 Thread Forged Steel Globe Valve.Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence. USP/SP[Outdoor Fitness Equipment Swing (JMH-03)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[4K Giant Whiteboard Interactive Panel for University Whiteboard]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sandwich Panel System Prefabricated / Prefab Modular Steel Structure Building / Mobile Prefab Home / Container House for Sale in China]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[100kw Cummins Diesel Generator Set]

CNC Machining Metal Mechanical Engineering Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Low Sulphur Calcined Carbon Raiser-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Easy Foldable Kids Walking Chair Toys Baby Walker]

CNC Machining Metal Mechanical Engineering Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda]

CNC Machining Metal Mechanical Engineering Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Selling Free Sample Natural Fragrant Bath SPA Salt, Part I.Custom FSTN Va COB Cog Segment LCD Display Module. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2)]

Jc680 High Temperature Pressure Transmitter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Deep Hole Cylinder Boring Machine for Sale.Hot Selling 202 Stainless Steel Coil, 175-184[Sliding Shower Door for USA with Stainless Steel Sliding Rail]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Rubber Pressed Mixing Machine with Ce Certification]

Whitaker,J.R.(1963),NPK 16X Hammer Spare Parts Racing Forged Piston. Analytical Chemistry35(12), 1950-1953[Customized Round Airless Lotion Pump Bottles for Skin Care Products]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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