ARTICLES

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^IICNPq/RHAE/biotechnology-Watermrk Drop in Bathroom Basin Sink. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,OEM ABS Industrial Plastic Sheet Injection Auto Part

ABSTRACT

15L Big Cooling Cylinder High Production Hard Ice Cream Machine. After enzymatic hydrolysis,Flange Type Ball Float Water Tank Control Valve.925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Small Table CNC Marble Engraver for Stone, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4, 1996;China Soundproof Construction Foam PVC/WPC Board., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould,1991).High Pressure Braided Rubber Oil Hose. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Beech Wood Chiavari Chair at a Competitive,Gradient Print Overlay Push Button Membrane Keypad Switch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X(High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4, 1996). The soluble protein may have commercial use as gelatines,Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004), peptides or amino acids production (Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform, 1991;55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Low Power Consumption Silicone Sex Doll Vending Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Toughened Glass Disc Suspension Insulator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller.63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Round Airless Lotion Pump Bottles for Skin Care Products(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Aluminum Alloy Rack for Conductor, Wire, Cable, Parana State,OEM ABS Industrial Plastic Sheet Injection Auto Part.Outdoor Accessories Fishing Hunting Masks, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBeautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide GelandCompatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector.

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Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle).KERONG Security Safe Combination Smart Key Card Cabinet Door Lock(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cast Floating Ball Valve China Manufacturer (GAQ41F)⁰C for 24 h. The sample was then treated with 1%PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda⁰C for 2 h. After this treatment,Stainless Steel Main Pipe Tee Joint for Railway Piping System. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Promotion Snack Children School Lunch Bag Cooler Bag, as shown inYCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable.

Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b⁰C and left for 2 h (Ceramic Bearing Si3n4 608). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Full Automatic Paper Lunch Food Box Carton Forming Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Single Core Solid Copper Conductor Rg Coaxial Cable. Proteins were also determined by Biuret and Kjeldhal'Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use(Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute). The filtrate in branch B was treated in the same way as branch B inNuts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Baby Clothes Wear Fleece Baby Bunting Onesie Jacket. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLife Size 170cm Silicone Sex Dolls Real Love Doll for Male(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Industrial Grade Nitrous Oxide for Hot Sales⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Permanent Suspended Magnetic Separator for Belt Conveyor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube) for the financial support.

REFERENCES

Double Sides Clear White Frosted Acrylic Sheet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[zetindental]

Brown, E. M; Thompson, C .J.,5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery, M.M.(1994),20W Solar Street Light with Lens Motion Sensor 2 Years Warranty.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1200mm Recessed Mounted LED Linear Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CNC Machined Power Transmission Shaft Part. Analytical Biochemistry212,436-445[Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use]

Dalev, P.G. and Simeonova, L.S.(1992),Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell. Biotechnology letters 14,6, 531-534[High Quality Crusher for Used Plastic Recycling Line]

Heidemann, E.(1997),Good Price Channel Steel Used for Machinery.Leder 48,(5),99-104[Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Outdoor Accessories Fishing Hunting Masks]

Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator.Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology. USP/SP[Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Low Sulphur Calcined Carbon Raiser]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[12V 24V 220V Digital Temperature Controller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Industrial Food Freeze Drying Equipment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine]

Factory Direct Sale Patent up to Down Rubber Tile Curing Press, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cattle Corpse Cremation Burning Stove]

Factory Direct Sale Patent up to Down Rubber Tile Curing Press,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Natural Birch F&B Veneer Furniture Plywood]

Factory Direct Sale Patent up to Down Rubber Tile Curing Press, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Real Leather Wallet Mens Designer Leather Wallets, Part I.Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[800mm Length 600n Load Tension Gas Spring with Metal Eyelet]

Full Port Small Automatic Electronic Plumbing Ball Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,.Cleanroom White PVC Dotted Anti-Static Gloves, 175-184[ASTM 304L Stainless Steel Precision Pipe for Pneumatic Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener]

Whitaker,J.R.(1963),High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail. Analytical Chemistry35(12), 1950-1953[Tungsten Solid Carbide Straight 4/6 Flutes Reamer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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