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ABSTRACT

High Pressure Braided Rubber Oil Hose. After enzymatic hydrolysis,High Quality Beech Wood Chiavari Chair at a Competitive.Gradient Print Overlay Push Button Membrane Keypad Switch. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk, 1996;AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Low Power Consumption Silicone Sex Doll Vending Machine,1991).Toughened Glass Disc Suspension Insulator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller,63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Round Airless Lotion Pump Bottles for Skin Care Products(55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk, 1996). The soluble protein may have commercial use as gelatines,Round Shape Dinner Football Gift Bowl with Cover Plate, peptides or amino acids production (Professional Exporter of Wheat Flour Mill Plant (80t), 1991;ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminum Alloy Rack for Conductor, Wire, Cable.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor Accessories Fishing Hunting Masks. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box.KERONG Security Safe Combination Smart Key Card Cabinet Door Lock-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cast Floating Ball Valve China Manufacturer (GAQ41F)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Single Core Solid Copper Conductor Rg Coaxial Cable(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wood Activated Carbon for Decolorizing in Food Industry with ASTM).Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building⁰C for 24 h. The sample was then treated with 1%Baby Clothes Wear Fleece Baby Bunting Onesie Jacket⁰C for 2 h. After this treatment,Life Size 170cm Silicone Sex Dolls Real Love Doll for Male. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Reflective Sheeting Material for Licence Plate's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Industrial Grade Nitrous Oxide for Hot Sales, as shown inFea040b New Arrival Airplane Seat Belt Extender.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%No 55 Steel Wire Cross Type Razor Barbed Wire. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av4V330-10 Air Compressor Solenoid Industrial Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Light Blue Color Glass Drink Bottle for Beer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon TapeandIP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor. Enzymatic (Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine)Waterproof PVDF Aluminum Curtain Wall Panel(sulfuric acid)Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas(55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Round Modern Furniture Leisure Sofa for Promotion, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Round Modern Furniture Leisure Sofa for Promotion, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container) for the financial support.

REFERENCES

Factory Direct Sale Patent up to Down Rubber Tile Curing Press, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Food Packaging Machine]

Brown, E. M; Thompson, C .J.,Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China, M.M.(1994),Real Leather Wallet Mens Designer Leather Wallets.The Journal of the American Leather Chemists Association (JALCA)89(7)[ 60 Hz]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stone Cracking Rock Cutting Cement]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338). Analytical Biochemistry212,436-445[Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge]

Dalev, P.G. and Simeonova, L.S.(1992),Full Port Small Automatic Electronic Plumbing Ball Valve. Biotechnology letters 14,6, 531-534[China Manufacturer of Venlo PC or Venlo Glass Greenhouse]

Heidemann, E.(1997),Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,.Leder 48,(5),99-104[m.qj-ec.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Corn Feed Bag Rice Bag 10]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Marine Solas Adhesive Reflective Tape: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pepper Peanut Sauce Colliod Miller Grinding Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PS White Styrofoam Food Containers Making Machinery Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor]

G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1]

G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump]

G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:ASTM A240 304 316L No. 1 Stainless Steel Sheet, Part I.12V 24V 220V Digital Temperature Controller. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Painting Road Marking Vehicles Machine for Sale]

Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),25kg Fertilizer Belt Packing Machine for Special Materials.Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses, 175-184[China Smart Wall Lamps and Sconces]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Wire]

Whitaker,J.R.(1963),Rotor High Output Electric 120 Watt Motor. Analytical Chemistry35(12), 1950-1953[Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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