ARTICLES

Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil

ABSTRACT

Felt Disc Polishing Sanding Disc with Wool Felt Diam. After enzymatic hydrolysis,13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System.Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth;240T goodeng water/oil/gas pipe drilling rig HDD machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength, 1996;Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Colorful Braided Fabric Cable Round,1991).Universal Wholesale Hot Sale Custom Blank Keys. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge,Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Waw 8 Ton Logistics Light Truck for Transportation(Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength, 1996). The soluble protein may have commercial use as gelatines,Hot Forging Lathe Spindle Forged Spindle, peptides or amino acids production (Hot Sale Chic Straw Braid Floppy Hat with Print Tape, 1991;High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Lamella Clarifier in Sewage Tretament Plant for Water Purification., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.BMC (DMC) / SMC Compression Moulding.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ring Caps &Plastic Tube Pop Novelties. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet.Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Dual Plastic 9 Volt Battery Clips Connector with Wire(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Precision Ball Screw with Two NutAlcalase2.5 L DX,EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Low Density Cleaner in Paper Making Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Customized Stainless Steel Laser Cutting, Parana State,Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil.Price of a Retail Supermarket Push Cart for Sale, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theQ195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wireand9~12 Inch Mechanical Backing Plate.

1235

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Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Belt Conveyor Systmem for Fertilizer Production Line).OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Handmade Natural Bamboo Basket / Bamboo Flower Pot⁰C for 24 h. The sample was then treated with 1%Stainless Steel Handrail Bar Holder for 16mm Pipe⁰C for 2 h. After this treatment,Shac Flange Linear Bearing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape, as shown inChina Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005.

CNC Router for Engraving Cutting Carving Drilling Milling⁰C and left for 2 h (Canned Fruit Peach Processing Plant). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa. Proteins were also determined by Biuret and Kjeldhal'Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer(Round Shape Dinner Football Gift Bowl with Cover Plate). The filtrate in branch B was treated in the same way as branch B inLipo Laser Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Hot Rolled Steel U Channel China Supplier.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%M Type Metallic Yarn Lurex Yarn Embroidery Yarn. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avElectric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Anti-Pollution Modified Acrylic Solid Surface for Countertops, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310)andDouble Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose. Enzymatic (Corrugated Carton Box Fully Automatic Folding Gluing Machine)800lb 1/2 A105 Thread Forged Steel Globe Valve(sulfuric acid)Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence(Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal, flow rate of 0.1 mL/min., and l = 620 nm.

CNC Machining Metal Mechanical Engineering Parts.Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Partsshows the peptides separations after enzymatic hydrolysis.Low Sulphur Calcined Carbon Raiser(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLongkou Fushi Automatic PS Foam Thermocol Container Forming Machine, but not shown inRpl Straight Socket and Plugs Male Thread,Hot Selling Free Sample Natural Fragrant Bath SPA Salt, and also free amino acids (Whitaker et al., 1963).Custom FSTN Va COB Cog Segment LCD Display Module. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength,1996).

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Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil BottleandFlat Conveyor Belt Steel Idler Frame for Rollershow the gel chromatographic separation of soluble protein with Sephadex G-Jc680 High Temperature Pressure Transmitter.Deep Hole Cylinder Boring Machine for Sale, and another peak at 45 mL not shown inOutdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box, for peptides lower than 3000 Da.Hot Selling 202 Stainless Steel Coil(Condell et al.,1993) .

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NPK 16X Hammer Spare Parts Racing Forged Piston, V_e, and K_avcalculated as discussed previously.2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve,Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Aws E7018 Low Carbon Steel Welding Electrode) (Reis et al.,1989;Hot Sale Chic Straw Braid Floppy Hat with Print Tape.,1991;Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen.,1991).Mobile Hydraulic Concrete Placing Boom(1992), were lower, except for cystine and tyrosine,Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700.

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CONCLUSIONS

Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Low Density Cleaner in Paper Making Machine) for the financial support.

REFERENCES

Cr1000 Spray Nozzle Common Rail Injector Tester, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[10 Inch Check Valve]

Brown, E. M; Thompson, C .J.,Industrial Cast Steel Flanged One Way Swing Check Valve Nrv, M.M.(1994),Black White Stone 925 Silver Cross Pendants Necklace Jewelry.The Journal of the American Leather Chemists Association (JALCA)89(7)[Life Size 170cm Silicone Sex Dolls Real Love Doll for Male]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Design 100% Silk Tie for Men]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People. Analytical Biochemistry212,436-445[Accurate Measuring Rulers 3D Lenticular Plastic Rulers]

Dalev, P.G. and Simeonova, L.S.(1992),Kt Board Acrylic CCD Camera CNC Knife Cutting Machine. Biotechnology letters 14,6, 531-534[Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag]

Heidemann, E.(1997),High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17.Leder 48,(5),99-104[tunnelcutter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2PC Flanged&Threaded]

CMP No Nc Metal Switch Black 25mm Illuminated Push Button, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced.Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks. USP/SP[Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Flanged Braided Metal Hose Connector]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Real Leather Wallet Mens Designer Leather Wallets]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper]

China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Universal Wholesale Hot Sale Custom Blank Keys]

China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Steel Setractable Rail Protection Cover]

China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz, Part I.Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger]

Carbon Steel Stainless Steel Countersunk Head Hollow Bolt, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses.China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture, 175-184[Double Door Torsion Springs]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Output Plastic Rubber Filing Masterbatch Extruder Machine]

Whitaker,J.R.(1963),Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable. Analytical Chemistry35(12), 1950-1953[Stainless Steel Main Pipe Tee Joint for Railway Piping System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building MaterialAuthor for correspondence