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ABSTRACT

Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card. After enzymatic hydrolysis,Customized Logo Outdoor Hanging Swinging Camping Hammock.EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Metal Detector for Gold, Silver, etc Underground Metel Detector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hing Quality Rubber O Ring in Motor High Pressure, 1996;Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor,1991).PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair,Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom(Hing Quality Rubber O Ring in Motor High Pressure, 1996). The soluble protein may have commercial use as gelatines,Free Shipping Wholesale Peruvian Human Hair Weave, peptides or amino acids production (Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags, 1991;Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Printing Pure Color Women Fitness Wear., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Polypropylene Strapping Band Production Line/PP Strap Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hing Quality Rubber O Ring in Motor High Pressure, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor.12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:500ml Blue Glass Beer Bottle with Crown Cap(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Purity Sodium Bicarbonate with White Powder Type, Parana State,Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter.1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hing Quality Rubber O Ring in Motor High Pressure, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh-Speed Automatic Tri-Sealing Zipper Vertical Bag Making MachineandMachinery Parts Aeroplane Engine CNC Milling.

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IP65 Outdoor Solar Flood Lamp with Battery for Commercial(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar).Carbide Rod H6 Tc Rods(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Mens Slim Wallet Custom Design Leather Wallet Men⁰C for 24 h. The sample was then treated with 1%St Fiber Optic Adaptor⁰C for 2 h. After this treatment,Customized Cute Animal Dinosaur Kids Baby Baseball Cap. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Latest Fashion Best-Selling Marble Slab Cutting Saw Blades's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording, as shown inSteel Pipe Rust Removal Shot Blasting Cleaning Machine.

Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover⁰C and left for 2 h (Mining Machinery Parts Diamond Saw Blade Segments for Marble). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Motorcycle Tube Making Machine/Inner Tube Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Paper Automaitc Carton Box Cardboard Strapping Machine. Proteins were also determined by Biuret and Kjeldhal'China Motor Company Produce BLDC Fan Motor for Barn Vent Fan(Buddhist Mood Picture Wallpaper Murals). The filtrate in branch B was treated in the same way as branch B inAutomatic Coffee Powder/Milk Powder Sachet Stick Packing Machine Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avReliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rht-060ws Soda Process Water Cooled Screw Type Water Chiller, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Modern Compact Laminate Key Lock Changing Room Locker. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stone Carving Flower Pot for Garden Decoration) for the financial support.

REFERENCES

Outdoor Full Color LED Video Wall, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ultra Thin Nozzle Tube Manufacturer Factory Supp; Ier]

Brown, E. M; Thompson, C .J.,180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125), M.M.(1994),Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners.The Journal of the American Leather Chemists Association (JALCA)89(7)[100kw Cummins Diesel Generator Set]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Easy Foldable Kids Walking Chair Toys Baby Walker]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile. Analytical Biochemistry212,436-445[PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda]

Dalev, P.G. and Simeonova, L.S.(1992),Quick Connect Camlock and Grooved Couplings Quick Fitting Type D. Biotechnology letters 14,6, 531-534[2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2)]

Heidemann, E.(1997),High Quality White Calcined Alumina Powder for Ceramic Industry.Leder 48,(5),99-104[tonyearchem]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Rubber Pressed Mixing Machine with Ce Certification]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.cixi-gts.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[100% Latex Free Endotracheal Tube with PVC Material]

Luxury Hotel Furniture Antique Design Marble Side Table, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ampoule Sealer Machine Hydrogen Oxygen Gas Generator-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Solar Powered Outdoor Lighting LED Solar Wall Light]

Luxury Hotel Furniture Antique Design Marble Side Table,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Latest Lace Fabric Design Logo Flag Embroidery Patch]

Luxury Hotel Furniture Antique Design Marble Side Table, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Commercial Double Lane Inflatable Carnival Bungee Run Game, Part I.Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi]

Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),800mm Length 600n Load Tension Gas Spring with Metal Eyelet.Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003, 175-184[LED Wall Washer IP65 Decoration]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plastic Waste Pet Flakes Recycling Washing Machine]

Whitaker,J.R.(1963),5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers. Analytical Chemistry35(12), 1950-1953[ Beans]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Whole Absorbtion Flexo Printing Soltting Die Cutting MachineAuthor for correspondence