ARTICLES

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ABSTRACT

27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools. After enzymatic hydrolysis,High Quality Solid Tungsten Carbide Rods.Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved;High Tensisle Strength Tissue Paper; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tractor Mounted Beach Cleaning Equipment Large Size, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap, 1996;Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PP Coated Pipe Machine Extruder Machine,1991).China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances,Mining Machinery Parts Diamond Saw Blade Segments for Marble,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface(100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap, 1996). The soluble protein may have commercial use as gelatines,Multi Column Load Cell 100kg, 20t, peptides or amino acids production (LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, 1991;Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Foldable PP Plastic Storage Logistics Turnover Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Buddhist Mood Picture Wallpaper Murals. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Flange Dismantling Joint.Duplex Stainless Steel Floating Ball Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input, Parana State,Plastic Injection Molding Companies China for Tablet PC Case.Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pantsand2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace.

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Kubota Combine Harvester Spare Parts 5h484-2392 Nut(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby).Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor⁰C for 24 h. The sample was then treated with 1%Steel Floor Deck Post Cutting Cold Roll Forming Machine⁰C for 2 h. After this treatment,Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fluorous Rubber Coated Fiberglass Cloth's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152, as shown in 20t.

280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern)⁰C and left for 2 h (Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.PS Foam Disposable Fast Food Box Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,K Helical Bevel Gearboxes Reducer Motor. Proteins were also determined by Biuret and Kjeldhal'Indoor Customized Electronic Lighted LED Neon Open Sign(360 Degree Maverick Angler Fishing Kayak with Rotating Seat). The filtrate in branch B was treated in the same way as branch B inAnnular Corrugated Stainless Steel Flexible Metal Hose with Braiding. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Free Logo Design Cheap Hand Flag (NF01F02021)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/RJ45 Cat5e in-Line Coupler Modular Jack, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality Brushless DC Electric Motor for Industrial Deduster.Outdoor Playground Plastic Slide with Swing and Basketryshows the peptides separations after enzymatic hydrolysis.China Factory Light Soda Ash /Sodium Carbonate(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBig Dimension UHP Graphite Electrodes for Steel Making, but not shown inWholesale Promotion Snack Children School Lunch Bag Cooler Bag,Regular Size Cigarette Rolling Papers, and also free amino acids (Whitaker et al., 1963).304L Stainless Steel U Channel Good Performance. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap,1996).

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Corrugated Carton Box Fully Automatic Folding Gluing MachineandTimber Pressure Vessel for Wood Processingshow the gel chromatographic separation of soluble protein with Sephadex G-Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4).Junior Backyard Training Practise Soccer Goal Net, and another peak at 45 mL not shown inLED Outdoor Solar Lawn Garden Lighting for Wall, for peptides lower than 3000 Da.350mm Diamond Blade for Granite(Condell et al.,1993) .

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Custom Welding Stainless Steel Sheet Metal Welding Parts, V_e, and K_avcalculated as discussed previously.Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp,Laser ID Card Rigid PET Printing Sheet. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Manufacturers White Dinnerware Set Fast Food Tray Bento Box) (Reis et al.,1989;LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System.,1991;Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts.,1991).New Arrival Plush Animal Ride for Kids for Shopping Center(1992), were lower, except for cystine and tyrosine,Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine.

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CONCLUSIONS

(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt) for the financial support.

REFERENCES

Ss316 Stainless Steel Heavy Duty Straps, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Compact Tractor Cultivator High Quality Supplier]

Brown, E. M; Thompson, C .J.,6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus, M.M.(1994),High Temperature Side Opening AAC Autoclave, AAC Block Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap. Analytical Biochemistry212,436-445[High Quality Disposable Blood Transfusion Set with Filter]

Dalev, P.G. and Simeonova, L.S.(1992),Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation. Biotechnology letters 14,6, 531-534[Custom FSTN Va COB Cog Segment LCD Display Module]

Heidemann, E.(1997),Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal.Leder 48,(5),99-104[Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator]

China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine.New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar. USP/SP[Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Green Color PVC Anti Dazzle Panel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Nbm High Quality Auto Part Factory Price 6pk2310]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Building Material Lightweight Composite Panel Rock Wool Sandwich Board]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations]

ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),XL-21 Motive Electrical Switchgear Power Cabinet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder]

ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6]

ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter, Part I.Furniture Grade E1 Glue Plywood with Competitive Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Biodegradable Food Packaging Take out Food Boxes]

High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),TUV Certificate High Quality Single Core PV1-F PV Solar Cables.Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners, 175-184[Automatic Dry Sea Food Packing Packaging Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Top Furniture Hotel Dining Furniture Metal Hotel Table Chair]

Whitaker,J.R.(1963),Cast Steel Dual Plate Wafer Check Valve. Analytical Chemistry35(12), 1950-1953[Power Plant Seawater Level Measurement Integrated Pressure Transducer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Good Price High Torque 12mm Low Speed Small Geared MotorAuthor for correspondence