ARTICLES

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ABSTRACT

Aws E7018 Low Carbon Steel Welding Electrode. After enzymatic hydrolysis,Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting.Electrical Bike Waterproof Power Connector. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass;Painted G80 Cargo Lashing Chain with Hooks; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Feeding Customized Multi Layers Shirts Cutting Machine, 1996;Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Portable Powerful Charger with Dual Pd Charger and Type-C Charger,1991).Flexible Vj Rigid Shaft Coupling for Pipe Flange. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting,Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers(Automatic Feeding Customized Multi Layers Shirts Cutting Machine, 1996). The soluble protein may have commercial use as gelatines,Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry, peptides or amino acids production (100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price, 1991;15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Speed Steel Mill Roll with High Quality., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2017 China Hot Sale Water Screen Movie Fountain. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Feeding Customized Multi Layers Shirts Cutting Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes.Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Delicate Handmade Round Natural Stone Chain Necklace, Parana State,Machinery Parts Aeroplane Engine CNC Milling.Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Automatic Feeding Customized Multi Layers Shirts Cutting Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNew Scba Breathing Apparatus Equipment with Ce CertificationandEmpty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer.

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Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter).Embossed Aluminium/ Aluminum Household Foil 8011-O(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021)⁰C for 24 h. The sample was then treated with 1%Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle⁰C for 2 h. After this treatment,Cross Hex Flange Screw. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Full Color Indoor Outdoor LED Curtain TV Display, as shown inSpark Resistant Construction Axial Fan with Fixed Pitch Blade.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pepper Peanut Sauce Colliod Miller Grinding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGasoline Rototiller, Cultivator, Tiller Model Gtm-6(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electronic Foldable for Patient Treatment Ent Unit Chair⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic Pushbutton Ultrasonic Spin Welding Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1.China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chickenshows the peptides separations after enzymatic hydrolysis.Wld8200 Auto Spray Paint Booth(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl, but not shown inPVDF Exterior Design Curved Aluminum Panel,Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10), and also free amino acids (Whitaker et al., 1963).Animal Carcass Incinerator Hospital Garbage Incinerator. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Feeding Customized Multi Layers Shirts Cutting Machine,1996).

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Laser Cut Sheet Metal Fiber Laser Cutting Machine, V_e, and K_avcalculated as discussed previously.Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring,Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (100 Liter Cooking Pots for Jam) (Reis et al.,1989;100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price.,1991;Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts.,1991).New Sexy Crystal Self Adhesive Resuable Nipple Cover(1992), were lower, except for cystine and tyrosine,Candy Box Laser Marking Engraving Machine Scm2000 180W 250W.

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CONCLUSIONS

130kw 7 Degrees Scroll Type Industrial Water Chiller. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Latest New Hho Engine Oil Carbon Remover) for the financial support.

REFERENCES

Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rubber Daylight Heat Platen Hydraulic Curing Vulcanizing Press Machine]

Brown, E. M; Thompson, C .J.,Factory Wholesale Melamine Garment Shop Furniture Design for Store, M.M.(1994),Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Waterjet 87K Intensifier Part Check Valve Body 020077-1]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Comfortable Men′s Winter Leather Snow Boots. Analytical Biochemistry212,436-445[500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality. Biotechnology letters 14,6, 531-534[Small Table CNC Marble Engraver for Stone]

Heidemann, E.(1997),Gcc Plant Ball Mill and Air Classifier Production Line.Leder 48,(5),99-104[Black Garlic Making Machine E]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[65GSM Woven Dyed Heavey Stretch Double Georgette Health Smooth Dress Fabric]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV]

Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Belt Conveyor Systmem for Fertilizer Production Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Plastic Material Handling Dolly Platform Hand Trolley Truck]

Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[500ml Blue Glass Beer Bottle with Crown Cap]

Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Linear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price, Part I.Digger Spring for Excavator Machinery. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip]

1.8ml Clear Sample Vials with Label and PP Caps, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Small Coil Compression Spring in High Quality.Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates, 175-184[Polishing Pads For Marble]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Chromium Oxide Green 99% for Construction and Plastics]

Whitaker,J.R.(1963),Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel. Analytical Chemistry35(12), 1950-1953[Fashion Men′s Large Dial Casual Quartz Watch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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