ARTICLES

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ABSTRACT

Building Material of Granite Ceramic Wall Tile for Wall Decoration. After enzymatic hydrolysis,3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine.Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop;Precision Customized CNC Machined Mild Steel Flange for Automobile; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Metal Container Packaging for Edible Oil Tin Cans Storage Boxes, 1996;Melee Custom Plastic Mould New Design., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter,1991).Copper-Pressed V-Type Expansion Pipe Joint. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber,White Desiccant Bag Silica Gel with Indicated Orange Bead,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Iron Wire Mesh Chain Link Mesh Security Fence(Metal Container Packaging for Edible Oil Tin Cans Storage Boxes, 1996). The soluble protein may have commercial use as gelatines,TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump, peptides or amino acids production (5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide, 1991;500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.360 Degree Maverick Angler Fishing Kayak with Rotating Seat.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Metal Container Packaging for Edible Oil Tin Cans Storage Boxes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Flower Embroidery Applique Patches.Big Bussiness Project Commerical Chicken Poultry Farm Buildings-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ceramic Bearing Si3n4 608(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Double Shaft Paper Cardboard Waste Plastic Shredder Machine).Nbm High Quality Auto Part Factory Price 6pk2310(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay⁰C for 24 h. The sample was then treated with 1%High Quality Mini LED Flood Light SMD Flood Light 50W⁰C for 2 h. After this treatment,Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Professional Manufacture Metal Medal Promotional Gift, as shown inBuilding Material of Granite Ceramic Wall Tile for Wall Decoration.

Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving⁰C and left for 2 h (Plastic Flange Housing Ucf310-115 for Electric Hand Tools). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Singlemode Su Upc Fiber Optic Fast Connector. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Roller Skating Sneakers Shoes for Children (8083). Proteins were also determined by Biuret and Kjeldhal'Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder(Rock Tunnel Boring Machine for Natural Gas Pipeline). The filtrate in branch B was treated in the same way as branch B inBreathable Leather Security Working Shoes Suppliers for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Electric Heating Industrial Jacketed Glass Reactor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%40X-1600X Biological Microscope Xsz-107V LED Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av6063 Aluminum Coil for Commercial Vehicle Floor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1200X1000X810 Strong Collapsible Plastic Containers for Storage⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Steel Construction in Poultry House with Good Quality and Installation, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic) for the financial support.

REFERENCES

Q235 Q345 Hot Rolled Channel Steel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mobile Phone LCD for iPhone 6s Touch Screen LCD Display]

Brown, E. M; Thompson, C .J.,OEM Factory New Girls Nuts Crunch Cheap Price, M.M.(1994),Hsac Shac Linear Guideway.The Journal of the American Leather Chemists Association (JALCA)89(7)[Prefabricated Steel Structure Beam Frame Factory Offer Commercial Building]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3D Adjustable American Market Cabinet Door Mini Hinge]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses. Analytical Biochemistry212,436-445[Factory Wholesale Melamine Garment Shop Furniture Design for Store]

Dalev, P.G. and Simeonova, L.S.(1992),Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10). Biotechnology letters 14,6, 531-534[Ltmg Classic Product 6 Ton Wheel Front Loader for Sale]

Heidemann, E.(1997),Pure Water Cutting Head Assy for Waterjet Cutting Machine.Leder 48,(5),99-104[High-Quality Lubrication]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib]

25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat.Latest Lace Fabric Design Logo Flag Embroidery Patch. USP/SP[275ml Frosted Clear Glass Drinking Bottle for Juice]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Forging Lathe Spindle Forged Spindle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[For Corolla]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Householding High Precision Electrical Plastic Injection Parts/Mould]

High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant]

High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[25kg Fertilizer Belt Packing Machine for Special Materials]

High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New H Type Battery Cage System for Layer Chicken Farm, Part I.Calcined Petroleum Coke as Best Recarburizer in Iron Industry. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Jc680 High Temperature Pressure Transmitter]

X15 Power Monitor Two Way Live Stage Monitor Speaker System, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Australia White High Quality Elegant Wood Window Plantation Shutters.Big Smart Hydroponic Planter Indoor Garden Light, 175-184[Carved Corbel Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment]

Whitaker,J.R.(1963),Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion. Analytical Chemistry35(12), 1950-1953[OEM Thermal Break Aluminum Profile for Aluminum Windows]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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