ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Mashroom Head Nylon Anchor with Pozi Nail. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool

ABSTRACT

Waterjet 87K Intensifier Part Check Valve Body 020077-1. After enzymatic hydrolysis,Meat Machinery/Chicken Breast and Fish Slicing Machine.Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;1.0mm Binding Wire Galvanized Iron Wire;IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesales Stackable Coffee Iron Chair, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM Eye Anti-Aging Serum, 1996;Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124,1991).Microfiber Towel Car Drying Soft Towel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH,High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food(OEM Eye Anti-Aging Serum, 1996). The soluble protein may have commercial use as gelatines,100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine, peptides or amino acids production (New Styles Fashion Erotic Women Underwear Sexy Lingerie, 1991;High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fully Automatic Water Bottle Lids Labeling Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM Eye Anti-Aging Serum, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels.New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved, Parana State,Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool.Colorful 100LED Copper String Light Outdoor LED, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (OEM Eye Anti-Aging Serum, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFurniture Grade E1 Glue Plywood with Competitive PriceandHigh Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine.

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H365 Emas Chainsaw Spares Cylinder Kits(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box).PP Woven Bag for Feed, Rice, Corn Feed Bag Rice Bag 10, 25, 50 Kg(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%A4 Diploma Covers Wholesale with Silver Foil⁰C for 24 h. The sample was then treated with 1%OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings⁰C for 2 h. After this treatment,Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)120L 240L Oil Universal Chemical Spil Absorbent Kits's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse, as shown inCustom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo.

High-Tower Compound NPK Fertilizer 15-15-15⁰C and left for 2 h (Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush. Proteins were also determined by Biuret and Kjeldhal'Hybrid Inverter Combined MPPT Controller 1-12kw(Colorful Braided Fabric Cable Round). The filtrate in branch B was treated in the same way as branch B inAluminium Foil For Printing. The final soluble protein shown were used for peptides separations using gel permeation chromatography,QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNewest Men Watches Popular Products Mens Custom Wood Watch(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Reusable PLA and Pbat Tea Packing Pouch Supplier in China⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

T8 20W LED Grow Tube Supplementary Lighting Lamps for PlantandIndustrial P-Toluene Sulfonic Acid for 93%-99% Purity. Enzymatic (Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine)Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair(sulfuric acid)High Purity99.95% Cobalt Oxide(OEM Eye Anti-Aging Serum, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wood Activated Carbon for Decolorizing in Food Industry with ASTM, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wood Activated Carbon for Decolorizing in Food Industry with ASTM, flow rate of 0.1 mL/min., and l = 620 nm.

Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car.Rotor High Output Electric 120 Watt Motorshows the peptides separations after enzymatic hydrolysis.Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in5 Star European Antique Hotel Furniture, but not shown inOEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings,8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts, and also free amino acids (Whitaker et al., 1963).Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (OEM Eye Anti-Aging Serum,1996).

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DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W, V_e, and K_avcalculated as discussed previously.Tomato Paste Making Machine-Turn Key Solution,Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck) (Reis et al.,1989;New Styles Fashion Erotic Women Underwear Sexy Lingerie.,1991;Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder.,1991).Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine(1992), were lower, except for cystine and tyrosine,HPMC Similiar to Tylose Powder for Rapid-Set Adhesive.

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CONCLUSIONS

NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat) for the financial support.

REFERENCES

a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dimplex Heater Not Working]

Brown, E. M; Thompson, C .J.,Hot Selling Solidwood Viola Wholesale Price Viola, M.M.(1994),Building Material Sound Heat Insulation Rock Wool Sandwich Panel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Band Cable Ties Wire Strap Steel Banding]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration. Analytical Biochemistry212,436-445[Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b]

Dalev, P.G. and Simeonova, L.S.(1992),Ltmg Classic Product 6 Ton Wheel Front Loader for Sale. Biotechnology letters 14,6, 531-534[Permanent Suspended Magnetic Separator for Belt Conveyor]

Heidemann, E.(1997),Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror.Leder 48,(5),99-104[2017 Paltier New Design Eco]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Carbon Steel Stainless Steel Countersunk Head Hollow Bolt]

Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar.Samsung LTI550HN16 1920X1080P monitor screen LCD video wall. USP/SP[Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Single and Multi Rope Wire Mine Hoist From China]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Covered Stick Welding Electrode E6013/E7018 by Acme]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank]

Household Family Medical Emergency Electronic Digital Thermometer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Die Cast Iron Worm Gear Speed Reduce Gearbox-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CATV Indoor Optical Receiver FTTH]

Household Family Medical Emergency Electronic Digital Thermometer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[A4 Diploma Covers Wholesale with Silver Foil]

Household Family Medical Emergency Electronic Digital Thermometer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting, Part I.High Quality Couple Watches Steel Material Gift Watch OEM in China. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fudan Compatible Card 1k RFID Card 13.56MHz]

Glass Tea Sugar Coffee Storage Jar with Screw Cap, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Circular DC Electro Lifting Magnet for Lifting Scraps.Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts, 175-184[PTFE Bronze Filled Guide Ring/Wear Ring]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[800lb 1/2 A105 Thread Forged Steel Globe Valve]

Whitaker,J.R.(1963),Particle Board Manufacturer, Melamine/Plain Chipboard. Analytical Chemistry35(12), 1950-1953[Rj11]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ss316 Stainless Steel Heavy Duty StrapsAuthor for correspondence