ARTICLES

Toughened Glass Disc Suspension Insulator

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Customized Round Airless Lotion Pump Bottles for Skin Care Products

ABSTRACT

Round Shape Dinner Football Gift Bowl with Cover Plate. After enzymatic hydrolysis,Professional Exporter of Wheat Flour Mill Plant (80t).ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine;Aluminum Alloy Rack for Conductor, Wire, Cable; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor Accessories Fishing Hunting Masks, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box, 1996;KERONG Security Safe Combination Smart Key Card Cabinet Door Lock., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cast Floating Ball Valve China Manufacturer (GAQ41F),1991).PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Main Pipe Tee Joint for Railway Piping System,Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Promotion Snack Children School Lunch Bag Cooler Bag(Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box, 1996). The soluble protein may have commercial use as gelatines,Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b, peptides or amino acids production (Full Automatic Paper Lunch Food Box Carton Forming Machine, 1991;Single Core Solid Copper Conductor Rg Coaxial Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Baby Clothes Wear Fleece Baby Bunting Onesie Jacket. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Life Size 170cm Silicone Sex Dolls Real Love Doll for Male.Reflective Sheeting Material for Licence Plate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Industrial Grade Nitrous Oxide for Hot Sales(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping StoveAlcalase2.5 L DX,Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Fire Alarm Detector 24VDC Reflective Beam Detector); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Automatic Mini Bag Spices Small Salt Packing Packaging Machine, Parana State,Customized Round Airless Lotion Pump Bottles for Skin Care Products.No 55 Steel Wire Cross Type Razor Barbed Wire, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStrain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600andRound Hole Carbon Steel Pipe Perforated for Filter.

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4V330-10 Air Compressor Solenoid Industrial Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial).Professional Various Standard Hot Sale Rotary Lab Single Round Punch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute⁰C for 24 h. The sample was then treated with 1%Light Blue Color Glass Drink Bottle for Beer⁰C for 2 h. After this treatment,Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, as shown inLithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack.

Waterproof PVDF Aluminum Curtain Wall Panel⁰C and left for 2 h (Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Round Modern Furniture Leisure Sofa for Promotion. Proteins were also determined by Biuret and Kjeldhal'Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket(Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation). The filtrate in branch B was treated in the same way as branch B inHot Sales Fine Cheap Custom Engraved Metal Medal. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sany Hg3333m Climbing Placing Boom.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Packaging Tray Vacuum Forming Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPermanent Suspended Magnetic Separator for Belt Conveyor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/20W Solar Street Light with Lens Motion Sensor 2 Years Warranty, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CNC Machined Power Transmission Shaft Part, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Dentist Used Metal Dental Steel Nickel Chrome with Beryllium AlloyandRepalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell. Enzymatic (Good Price Channel Steel Used for Machinery)Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator(sulfuric acid)Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology(Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount, flow rate of 0.1 mL/min., and l = 620 nm.

Factory Direct Sale Patent up to Down Rubber Tile Curing Press.Stainless Steel DIN7981 Pan Head Self Tapping Screwshows the peptides separations after enzymatic hydrolysis.Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus, but not shown inNew Arrival Plush Animal Ride for Kids for Shopping Center,Real Leather Wallet Mens Designer Leather Wallets, and also free amino acids (Whitaker et al., 1963).Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box,1996).

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Indoor Load Break Switch Isolating SwitchandSTPP Sodium Tripolyphosphate for Detergent / Ceramic / Foodshow the gel chromatographic separation of soluble protein with Sephadex G-Full Port Small Automatic Electronic Plumbing Ball Valve.Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,, and another peak at 45 mL not shown inNordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate, for peptides lower than 3000 Da.Cleanroom White PVC Dotted Anti-Static Gloves(Condell et al.,1993) .

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CONCLUSIONS

12V 24V 220V Digital Temperature Controller. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fire Alarm Detector 24VDC Reflective Beam Detector) for the financial support.

REFERENCES

Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[316 Steam Flexible Metal Hose]

Brown, E. M; Thompson, C .J.,25kg Fertilizer Belt Packing Machine for Special Materials, M.M.(1994),Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses.The Journal of the American Leather Chemists Association (JALCA)89(7)[120ml Glass Perfume Bottles for Designer Perfume]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rotor High Output Electric 120 Watt Motor. Analytical Biochemistry212,436-445[China Quality Heavy Duty Globe Control Valve]

Dalev, P.G. and Simeonova, L.S.(1992),Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby. Biotechnology letters 14,6, 531-534[2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels]

Heidemann, E.(1997),Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed.Leder 48,(5),99-104[High Brightness Blue Color IP20 SMD5050 Chip 60LEDs 14.4W DC12V LED Strip]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Q3210 Vibratory Polishing Shot Blasting Machine]

Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine.Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile. USP/SP[Building Decoration Material Acrylic Dispersant for Coating]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Line Scan Uvss System Portable Under Vehicle Surveillance]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rpl Straight Socket and Plugs Male Thread: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized Logo Outdoor Hanging Swinging Camping Hammock]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PV&Sv Dual LED Display UV Coating Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Candy Box Laser Marking Engraving Machine Scm2000 180W 250W]

46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM Metal Stamping Stainless Steel Parts with CNC Machining-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Supply Straight Ball Joint Bearing 02b04]

46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Cute Animal Dinosaur Kids Baby Baseball Cap]

46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ss Wafer Type Check Valve Dn32 Dn40 Dn50, Part I.2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High-Tower Compound NPK Fertilizer 15-15-15]

Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera.WPC Wood Plastic Board Equipment Extruder Machine, 175-184[99% Purity Peptides Powder Epitalon/Epithalon (10mg/Vial) 307297-39-8 for Anti Aging]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[4.0MP Ahd Tvi CVBS HD CCTV Camera Module]

Whitaker,J.R.(1963),Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS. Analytical Chemistry35(12), 1950-1953[Precision Ball Screw with Two Nut]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Wood Fireplace Double Skin Chimney Flue PipesAuthor for correspondence