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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Reflective Sheeting Material for Licence Plate. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Industrial Grade Nitrous Oxide for Hot Sales

ABSTRACT

Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove. After enzymatic hydrolysis,Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap.Fire Alarm Detector 24VDC Reflective Beam Detector. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle;Automatic Mini Bag Spices Small Salt Packing Packaging Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.No 55 Steel Wire Cross Type Razor Barbed Wire, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (4V330-10 Air Compressor Solenoid Industrial Valve, 1996;Professional Various Standard Hot Sale Rotary Lab Single Round Punch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute,1991).Light Blue Color Glass Drink Bottle for Beer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box,IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine(4V330-10 Air Compressor Solenoid Industrial Valve, 1996). The soluble protein may have commercial use as gelatines,Waterproof PVDF Aluminum Curtain Wall Panel, peptides or amino acids production (Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas, 1991;Round Modern Furniture Leisure Sofa for Promotion, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sany Hg3333m Climbing Placing Boom.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plastic Packaging Tray Vacuum Forming Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (4V330-10 Air Compressor Solenoid Industrial Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Permanent Suspended Magnetic Separator for Belt Conveyor.Double Sides Clear White Frosted Acrylic Sheet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

20W Solar Street Light with Lens Motion Sensor 2 Years WarrantyAlcalase2.5 L DX,CNC Machined Power Transmission Shaft Part, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Good Price Channel Steel Used for Machinery(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator, Parana State,Industrial Grade Nitrous Oxide for Hot Sales.Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (4V330-10 Air Compressor Solenoid Industrial Valve, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIndustrial Grade Nitrous Oxide for Hot SalesandCustmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall.

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Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine).Factory Direct Sale Patent up to Down Rubber Tile Curing Press(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China⁰C for 24 h. The sample was then treated with 1%Real Leather Wallet Mens Designer Leather Wallets⁰C for 2 h. After this treatment,Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Full Port Small Automatic Electronic Plumbing Ball Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,, as shown inElectric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W.

Cleanroom White PVC Dotted Anti-Static Gloves⁰C and left for 2 h (Practical Edible Silicone Kitchenware (YB-ty-29)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Marine Solas Adhesive Reflective Tape. Proteins were also determined by Biuret and Kjeldhal'G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room(Shac Flange Linear Bearing). The filtrate in branch B was treated in the same way as branch B inJd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%ASTM A240 304 316L No. 1 Stainless Steel Sheet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av12V 24V 220V Digital Temperature Controller(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.25kg Fertilizer Belt Packing Machine for Special Materials⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rotor High Output Electric 120 Watt Motor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide StrapandFlexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby. Enzymatic (Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed)Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine(sulfuric acid)Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile(4V330-10 Air Compressor Solenoid Industrial Valve, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Rpl Straight Socket and Plugs Male Thread, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Rpl Straight Socket and Plugs Male Thread, flow rate of 0.1 mL/min., and l = 620 nm.

46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall.E26 E14 E17 E17 E39 Lamp Cap Go No Go Gaugeshows the peptides separations after enzymatic hydrolysis.OEM Metal Stamping Stainless Steel Parts with CNC Machining(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inUnbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp, but not shown in100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth,Ss Wafer Type Check Valve Dn32 Dn40 Dn50, and also free amino acids (Whitaker et al., 1963).2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (4V330-10 Air Compressor Solenoid Industrial Valve,1996).

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Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS, V_e, and K_avcalculated as discussed previously.Neoprene Elbow Support,Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera) (Reis et al.,1989;Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas.,1991;Professional Various Standard Hot Sale Rotary Lab Single Round Punch.,1991).CNC Produce Medical Machining Custom Metal Milling(1992), were lower, except for cystine and tyrosine,Multi Sensor Nilitary Surveillance Thermal Imaging Security camera.

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CONCLUSIONS

Custom Metal Stamping Welding Spare Parts for Farming. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell) for the financial support.

REFERENCES

Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Astm 316 Stainless Steel Tube]

Brown, E. M; Thompson, C .J.,Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation, M.M.(1994),275ml Frosted Clear Glass Drinking Bottle for Juice.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customized Double Acting Hydraulic Cylinder for Excavator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price. Analytical Biochemistry212,436-445[Professional Manufacturer Stainless Steel Floor Grating]

Dalev, P.G. and Simeonova, L.S.(1992),Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs. Biotechnology letters 14,6, 531-534[Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant]

Heidemann, E.(1997),Bolts and Nuts Set Brass Fasteners Double Hex Bolt.Leder 48,(5),99-104[Injectable Facial Filler]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Good Price Channel Steel Used for Machinery]

Life Size 170cm Silicone Sex Dolls Real Love Doll for Male, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Direct Sales Kids Boy Cotton Children T Shirt.Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener. USP/SP[CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PP/Pet Woven Geotextile for Road Construction]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic Injection Molding Companies China for Tablet PC Case]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1650mm*80mm Non-Metalic Materials Laser Tube]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant]

Light Blue PVC Plastic Sheet and PVC Film, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap]

Light Blue PVC Plastic Sheet and PVC Film,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush]

Light Blue PVC Plastic Sheet and PVC Film, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Metric Female Cone Hydraulic Hose Fitting, Part I.Hot Sale Dental Material Dental PMMA Block From China. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Cmyk Printing Cake Gift Packing Box with Lid]

Plastic Waste Pet Flakes Recycling Washing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Deposited Stainless Steel Rivet Nut with Knurled.High Quality Stainless Steel Mechanical Filter Hosing, 175-184[Soda Ash Dense 99.5% Na2co3 Sodium Carbonate]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hing Quality Rubber O Ring in Motor High Pressure]

Whitaker,J.R.(1963),Paper New Year Greeting Cards Wedding Invitations Cards. Analytical Chemistry35(12), 1950-1953[Hotel Card Door Lock Access Control Safe Door Lock]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin CanAuthor for correspondence