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ABSTRACT

Seasoned Cast Iron South Africa Three Legged Potjie Pot. After enzymatic hydrolysis,1.0g Water and Air Ozone Generator with Silicone Tube.Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;16A/24A High Speed Wire Cable Braiding Machine;Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (20 Inch 48V 750W Fat Tire Electric Bike with Double Seat, 1996;Agriculture Apple Orchard Reflective Pet Film., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04),1991).Small Boat Deck Knuckle Boom Ship Pedestal Crane. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,115mm Segmented Saw Blade for Concrete Cutting,Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Round Transparent Glass 750ml Liquor Glass Bottle(20 Inch 48V 750W Fat Tire Electric Bike with Double Seat, 1996). The soluble protein may have commercial use as gelatines,3D Creative Design Metal Medal Promotion Gift of Sport, peptides or amino acids production (Ad-L13 Black Perfume Plastics Vial Bottle 10ml, 1991;Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Universal Mirco Forklift Car Bus Truck Seats Switch Sensors. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (20 Inch 48V 750W Fat Tire Electric Bike with Double Seat, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder.Mexico Single Expansion Anchor with YZP-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Air Purification Activated Alumina(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Good Price Channel Steel Used for Machinery).Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Resistan UV Grades PP Non Woven Fabric⁰C for 24 h. The sample was then treated with 1%New Scba Breathing Apparatus Equipment with Ce Certification⁰C for 2 h. After this treatment,FRP Pultruded Pipe or GRP Profile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)6090 CO2 Sealed Glass Cooling Laser Cutting Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator, as shown inCNC Milling Machinery Parts From OEM Manufacturer/ Auto Part.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMetal Animal Longhorn Skull Pendant Longhorn Skull Charm(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Output Plastic Rubber Filing Masterbatch Extruder Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Precision Mold Manufacturer Plastic Injection Mould, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Tefude Feeding and Packaging Line for Food with Tray. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Whole Absorbtion Flexo Printing Soltting Die Cutting Machine) for the financial support.

REFERENCES

Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[12pcs Plastic Prepared Microscope Slides]

Brown, E. M; Thompson, C .J.,Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine, M.M.(1994),CMP No Nc Metal Switch Black 25mm Illuminated Push Button.The Journal of the American Leather Chemists Association (JALCA)89(7)[Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Leaf Shape Pearl Jewelry Wedding Tiara]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. Analytical Biochemistry212,436-445[Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards]

Dalev, P.G. and Simeonova, L.S.(1992),Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil. Biotechnology letters 14,6, 531-534[Cold Drawn D2 H13 Alloy Steel Turning Roll]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Metal Polishing Disc]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[20W Solar Street Light with Lens Motion Sensor 2 Years Warranty]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cnc Router Pantograph]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve]

240T goodeng water/oil/gas pipe drilling rig HDD machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Competitive Price Sodium Gluconate as Concrete Admixture]

240T goodeng water/oil/gas pipe drilling rig HDD machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cr1000 Spray Nozzle Common Rail Injector Tester]

240T goodeng water/oil/gas pipe drilling rig HDD machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength, Part I.Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[24 Hours Self-Service Water ATM Machine Automatic RO Water Vending Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple]

Whitaker,J.R.(1963),Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30. Analytical Chemistry35(12), 1950-1953[Custom Good Quality Outdoor Music Wireless Cooler Speaker Box]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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