ARTICLES

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ABSTRACT

Fluorous Rubber Coated Fiberglass Cloth. After enzymatic hydrolysis,Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152.280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PS Foam Disposable Fast Food Box Making Machine;K Helical Bevel Gearboxes Reducer Motor; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Indoor Customized Electronic Lighted LED Neon Open Sign, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar, 1996;Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Free Logo Design Cheap Hand Flag (NF01F02021),1991).125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X,RJ45 Cat5e in-Line Coupler Modular Jack,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture(Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar, 1996). The soluble protein may have commercial use as gelatines,Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper, peptides or amino acids production (Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052, 1991;High Quality Disposable Blood Transfusion Set with Filter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Brushless DC Electric Motor for Industrial Deduster. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Factory Light Soda Ash /Sodium Carbonate.Regular Size Cigarette Rolling Papers-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:304L Stainless Steel U Channel Good Performance(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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New Arrival Plush Animal Ride for Kids for Shopping Center(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Tomato Paste Making Machine-Turn Key Solution).Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine⁰C for 24 h. The sample was then treated with 1%Ss316 Stainless Steel Heavy Duty Straps⁰C for 2 h. After this treatment,6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Temperature Side Opening AAC Autoclave, AAC Block Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap, as shown inPrinting Pure Color Women Fitness Wear.

Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation⁰C and left for 2 h ( Diatomite Earth Beer Filter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine. Proteins were also determined by Biuret and Kjeldhal'New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar(Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine). The filtrate in branch B was treated in the same way as branch B inASTM 201 304 316 321 310S 904L 430 Cold/Hot Rolled Stainless Steel Sheet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Green Color PVC Anti Dazzle Panel.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avXL-21 Motive Electrical Switchgear Power Cabinet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Furniture Grade E1 Glue Plywood with Competitive Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn, pH 3.25., mobile phase B: buffer solution of 0.01 mol/TUV Certificate High Quality Single Core PV1-F PV Solar Cables, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (350mm Diamond Blade for Granite) for the financial support.

REFERENCES

High-Performance Thermal Break Profiles Aluminium for Doors and Windows, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Abec5 Bearings]

Brown, E. M; Thompson, C .J.,Design Luxury Moon Cake Box with Hot Stamp Logo, M.M.(1994),Standard Industrial Double Nut Ball Screw Dfu Series.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter. Analytical Biochemistry212,436-445[Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump]

Dalev, P.G. and Simeonova, L.S.(1992),Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card. Biotechnology letters 14,6, 531-534[PP Coated Pipe Machine Extruder Machine]

Heidemann, E.(1997),Customized Logo Outdoor Hanging Swinging Camping Hammock.Leder 48,(5),99-104[China Hot Sale DIN7505 Pozi Drive Double Flat Head Zinc Plated Chipboard Screws]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[100 Liter Cooking Pots for Jam]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Temperature Side Opening AAC Autoclave]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Acrylic Panel Capacitive Membrane Keypad Switch for Digital Safe Lock]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Waterjet 87K Intensifier Part Check Valve Body 020077-1]

Metal Detector for Gold, Silver, etc Underground Metel Detector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hing Quality Rubber O Ring in Motor High Pressure-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine]

Metal Detector for Gold, Silver, etc Underground Metel Detector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Small Table CNC Marble Engraver for Stone]

Metal Detector for Gold, Silver, etc Underground Metel Detector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe, Part I.Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof]

PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair.Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc, 175-184[Customized Eco-Friendly Reusable Stainless Steel Drinking Straws]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ad-L13 Black Perfume Plastics Vial Bottle 10ml]

Whitaker,J.R.(1963),Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom. Analytical Chemistry35(12), 1950-1953[Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Plastic Mining Biaxial Geogrid Similar to Tensar Mining GridAuthor for correspondence