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ABSTRACT

Pure Water Cutting Head Assy for Waterjet Cutting Machine. After enzymatic hydrolysis,Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat.Latest Lace Fabric Design Logo Flag Embroidery Patch. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken;High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New H Type Battery Cage System for Layer Chicken Farm, 1996;Calcined Petroleum Coke as Best Recarburizer in Iron Industry., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (X15 Power Monitor Two Way Live Stage Monitor Speaker System,1991).Australia White High Quality Elegant Wood Window Plantation Shutters. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Big Smart Hydroponic Planter Indoor Garden Light,Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire(New H Type Battery Cage System for Layer Chicken Farm, 1996). The soluble protein may have commercial use as gelatines,Hot Selling Custom Sheet Metal Fabrication, peptides or amino acids production (15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s, 1991;Powder Coating Paint Lines Systems for Factory /Plants, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mini Pencil Tag with Lanyard (YS211).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-100% Latex Free Endotracheal Tube with PVC Material. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New H Type Battery Cage System for Layer Chicken Farm, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Neodymium Magnet Pot Assembly with Strong Weight.Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Manufacturer of Venlo PC or Venlo Glass Greenhouse(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Householding High Precision Electrical Plastic Injection Parts/Mould, Parana State,Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10).Fashion Women Hair Accessories Hair Bow Headband for Girls, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (New H Type Battery Cage System for Layer Chicken Farm, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEnt Treatment Medical Chair with Foot Controland Noiseless) Cracking Agent (SCA) for Breaking Rocks.

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Round Hole Carbon Steel Pipe Perforated for Filter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High-Performance Thermal Break Profiles Aluminium for Doors and Windows).Professional Manufacturer Stainless Steel Floor Grating(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels⁰C for 24 h. The sample was then treated with 1%Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose⁰C for 2 h. After this treatment,Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Brand New Forklift with Double Front pneumatic Tire's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope, as shown in Compost Turner Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%4K Giant Whiteboard Interactive Panel for University Whiteboard. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic Mining Biaxial Geogrid Similar to Tensar Mining Grid(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Practical Edible Silicone Kitchenware (YB-ty-29), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controllerand4 Inch Quarter Round PCD Diamond Grinding Cup Wheel. Enzymatic (High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine)Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG)(sulfuric acid)Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl(New H Type Battery Cage System for Layer Chicken Farm, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48), flow rate of 0.1 mL/min., and l = 620 nm.

Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump.High Quality Iron Wire Mesh Chain Link Mesh Security Fenceshows the peptides separations after enzymatic hydrolysis.China Cube Ice Maker Parts / Ice Machines Maker Cube(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Brushless DC Electric Motor for Industrial Deduster, but not shown inBiogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment,Aluminium/Aluminum Casement Window with Break Bridge (FT-W108), and also free amino acids (Whitaker et al., 1963).3D Adjustable American Market Cabinet Door Mini Hinge. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New H Type Battery Cage System for Layer Chicken Farm,1996).

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CONCLUSIONS

Garden Furniture Sofa Rattan Modular Corner Set with Cushions. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts) for the financial support.

REFERENCES

Stainless Steel Water Cream Bulk Storage Tank, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[304 304L 1.4301 Decorative Rose Gold Finished Stainless Steel Sheet]

Brown, E. M; Thompson, C .J.,Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR), M.M.(1994),Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants.The Journal of the American Leather Chemists Association (JALCA)89(7)[LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mobile Hydraulic Concrete Placing Boom]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Seasoned Cast Iron South Africa Three Legged Potjie Pot. Analytical Biochemistry212,436-445[Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting]

Dalev, P.G. and Simeonova, L.S.(1992),1.0g Water and Air Ozone Generator with Silicone Tube. Biotechnology letters 14,6, 531-534[New Arrival Amazon Hot Style Printed Slip Dress for Women Summer]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New H Type Battery Cage System for Layer Chicken Farm]

Hsac Shac Linear Guideway, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),16A/24A High Speed Wire Cable Braiding Machine.Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot. USP/SP[Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Latest New Hho Engine Oil Carbon Remover]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Metal Casting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil]

20 Inch 48V 750W Fat Tire Electric Bike with Double Seat, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Agriculture Apple Orchard Reflective Pet Film-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Tefude Feeding and Packaging Line for Food with Tray]

20 Inch 48V 750W Fat Tire Electric Bike with Double Seat,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum]

20 Inch 48V 750W Fat Tire Electric Bike with Double Seat, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04), Part I.Small Boat Deck Knuckle Boom Ship Pedestal Crane. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture]

115mm Segmented Saw Blade for Concrete Cutting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit.China Round Transparent Glass 750ml Liquor Glass Bottle, 175-184[Fungicide With Chlorothalonil]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush]

Whitaker,J.R.(1963),3D Creative Design Metal Medal Promotion Gift of Sport. Analytical Chemistry35(12), 1950-1953[Good Quality Hot Tube8 Japanese Stainless Steel Tube 666]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming MachineAuthor for correspondence