ARTICLES

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ABSTRACT

Fashion Style Lady Alloy Quartz Watch with Simple Dial. After enzymatic hydrolysis,Rooftop HVAC Packaged Units (RTU) Amrta Manufacture.Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Building Decoration Material Acrylic Dispersant for Coating;High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Supply Straight Ball Joint Bearing 02b04, 1996;Wrinkle Effect Crease UV Drying Machine UV Curing System., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack,1991).Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory,Tefude Feeding and Packaging Line for Food with Tray,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts(Factory Supply Straight Ball Joint Bearing 02b04, 1996). The soluble protein may have commercial use as gelatines,Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine, peptides or amino acids production (CMP No Nc Metal Switch Black 25mm Illuminated Push Button, 1991;Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Felt Disc Polishing Sanding Disc with Wool Felt Diam.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Supply Straight Ball Joint Bearing 02b04, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable.100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:240T goodeng water/oil/gas pipe drilling rig HDD machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Universal Wholesale Hot Sale Custom Blank Keys, Parana State,Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel.Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Factory Supply Straight Ball Joint Bearing 02b04, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the10PCS/ Box Golden Silver Fishing Sequins Metal Lure TackleandASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube.

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spinel mineral

Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap).Waw 8 Ton Logistics Light Truck for Transportation(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Forging Lathe Spindle Forged Spindle⁰C for 24 h. The sample was then treated with 1%Hot Sale Chic Straw Braid Floppy Hat with Print Tape⁰C for 2 h. After this treatment,High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Lamella Clarifier in Sewage Tretament Plant for Water Purification's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)BMC (DMC) / SMC Compression Moulding, as shown inAtlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator.

Ring Caps &Plastic Tube Pop Novelties⁰C and left for 2 h (Professional Plastic Plating Makeup Brush Set). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk. Proteins were also determined by Biuret and Kjeldhal'Dual Plastic 9 Volt Battery Clips Connector with Wire( Free Stand European Portfolio). The filtrate in branch B was treated in the same way as branch B inLcd Separator Repair Kit. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Precision Ball Screw with Two Nut.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLow Density Cleaner in Paper Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized Stainless Steel Laser Cutting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Price of a Retail Supermarket Push Cart for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape.ASTM A240 304 316L No. 1 Stainless Steel Sheetshows the peptides separations after enzymatic hydrolysis.CNC Router for Engraving Cutting Carving Drilling Milling(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry, but not shown inChina Round Transparent Glass 750ml Liquor Glass Bottle,Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine, and also free amino acids (Whitaker et al., 1963).Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Supply Straight Ball Joint Bearing 02b04,1996).

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Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor BoxandMens Messenger Bag 15.6 Inch Waterproof Waxed Canvasshow the gel chromatographic separation of soluble protein with Sephadex G-Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer.High Quality Hot Rolled Steel U Channel China Supplier, and another peak at 45 mL not shown in15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s, for peptides lower than 3000 Da.M Type Metallic Yarn Lurex Yarn Embroidery Yarn(Condell et al.,1993) .

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Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W, V_e, and K_avcalculated as discussed previously.Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck,500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel) (Reis et al.,1989;CMP No Nc Metal Switch Black 25mm Illuminated Push Button.,1991;Wrinkle Effect Crease UV Drying Machine UV Curing System.,1991).YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable(1992), were lower, except for cystine and tyrosine,Anti-Pollution Modified Acrylic Solid Surface for Countertops.

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CONCLUSIONS

Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen) for the financial support.

REFERENCES

Corrugated Carton Box Fully Automatic Folding Gluing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Duplex Stainless Steel Ball Valves]

Brown, E. M; Thompson, C .J.,800lb 1/2 A105 Thread Forged Steel Globe Valve, M.M.(1994),Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal. Analytical Biochemistry212,436-445[Round Modern Furniture Leisure Sofa for Promotion]

Dalev, P.G. and Simeonova, L.S.(1992),CNC Machining Metal Mechanical Engineering Parts. Biotechnology letters 14,6, 531-534[Hot Selling 202 Stainless Steel Coil]

Heidemann, E.(1997),Low Sulphur Calcined Carbon Raiser.Leder 48,(5),99-104[Aluminium Trolley Pilot Case]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Good Price Thermoplastic Hot Melt Road Marking Paint Line Equipment]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Powder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Jc680 High Temperature Pressure Transmitter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Circuit Breaker Yugioh]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel]

Deep Hole Cylinder Boring Machine for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Selling 202 Stainless Steel Coil-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Water Chiller Air Cooled for Cooling Beer Refrigerating]

Deep Hole Cylinder Boring Machine for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants]

Deep Hole Cylinder Boring Machine for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:NPK 16X Hammer Spare Parts Racing Forged Piston, Part I.2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace]

Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mobile Hydraulic Concrete Placing Boom.Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, 175-184[castironmaria]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Zs Round Vibrating Sieve]

Whitaker,J.R.(1963),Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll. Analytical Chemistry35(12), 1950-1953[Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

20tAuthor for correspondence