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^IICNPq/RHAE/biotechnology-Salon Equipment Hair Clipper. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Flat Conveyor Belt Steel Idler Frame for Roller

ABSTRACT

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Key words;Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring;Chromium Oxide Green 99% for Construction and Plastics; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Zs Round Vibrating Sieve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Tactile Paving Blind Road Stud, 1996;70L Lab Use Refrigerated Circulator Chiller Factory Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500),1991).Roller Hearth Heating Furnace for Gas Cylindrer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,custom design MIFARE DESFire EV2 2K plastic RFID loyalty card,Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hotel Card Door Lock Access Control Safe Door Lock(Stainless Steel Tactile Paving Blind Road Stud, 1996). The soluble protein may have commercial use as gelatines,Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer, peptides or amino acids production (Stainless Steel DIN7981 Pan Head Self Tapping Screw, 1991;19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Dark Green Decorative Garden Welded Wire Mesh Fencing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Csm DIY Microscope for Kids/Toy Microscope. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Tactile Paving Blind Road Stud, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Oak Veneer Laminated Plywood for Decoration.China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Precision Investment Casting Stainless Steel 304 Wire Rope Hook(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Zs Round Vibrating Sieve).AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Die Casting Aluminium Product for Connector Usage⁰C for 24 h. The sample was then treated with 1%Stainless Steel Balustrade/Pillar/Column⁰C for 2 h. After this treatment,Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PP/Pet Woven Geotextile for Road Construction's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Lamella Clarifier in Industrial Wastewater Treatment, as shown inFlexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby.

FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering⁰C and left for 2 h ( 20t). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights. Proteins were also determined by Biuret and Kjeldhal'Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick(Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop). The filtrate in branch B was treated in the same way as branch B inBuy Windows 7 Key Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%IS Type Cast Iron Chemical Industrial Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale CNC Router Machine for Wood Cutting (1325)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/50g Body Luxury King Crown Cosmetic Containers, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Quaity China Supplier Truck Trailer Auto Car Accessories. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Women′s Suit in Reasonable Price) for the financial support.

REFERENCES

Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Sell Pneumatic Electric Framing Nailer Paper Strip Nail Gun]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Minimalist Slim Real Leather Front Pocket Card Wallet. Analytical Biochemistry212,436-445[180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125)]

Dalev, P.G. and Simeonova, L.S.(1992),Natural Cleaning Konjak Sponge. Biotechnology letters 14,6, 531-534[High Quality Disposable Blood Transfusion Set with Filter]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom FSTN Va COB Cog Segment LCD Display Module]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Redsun H Series Heavy Duty Gear Units]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Nbm High Quality Auto Part Factory Price 6pk2310]

NBR Latex Adhesive for Glove, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cheap Wooden House Zinc Plated Angle Bracket-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations]

NBR Latex Adhesive for Glove,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder]

NBR Latex Adhesive for Glove, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head, Part I.Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Biodegradable Food Packaging Take out Food Boxes]

Whitaker,J.R.(1963),500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet. Analytical Chemistry35(12), 1950-1953[Top Furniture Hotel Dining Furniture Metal Hotel Table Chair]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Power Plant Seawater Level Measurement Integrated Pressure TransducerAuthor for correspondence