ARTICLES

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ABSTRACT

STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food. After enzymatic hydrolysis,100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine.New Styles Fashion Erotic Women Underwear Sexy Lingerie. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fully Automatic Water Bottle Lids Labeling Machine, 1996;2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera,1991).Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fused Silica Quartz Lump 5-50mm for Refractory Materials,Cold Drawn D2 H13 Alloy Steel Turning Roll,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat(Fully Automatic Water Bottle Lids Labeling Machine, 1996). The soluble protein may have commercial use as gelatines,Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks, peptides or amino acids production (High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved, 1991;Colorful 100LED Copper String Light Outdoor LED, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;H365 Emas Chainsaw Spares Cylinder Kits., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PP Woven Bag for Feed, Rice, Corn Feed Bag Rice Bag 10, 25, 50 Kg.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-A4 Diploma Covers Wholesale with Silver Foil. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fully Automatic Water Bottle Lids Labeling Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings.Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:120L 240L Oil Universal Chemical Spil Absorbent Kits(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine⁰C and left for 2 h (Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Purity99.95% Cobalt Oxide. Proteins were also determined by Biuret and Kjeldhal'Wood Activated Carbon for Decolorizing in Food Industry with ASTM(Gcc Plant Ball Mill and Air Classifier Production Line). The filtrate in branch B was treated in the same way as branch B inCo2 Mig Wire High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/6000 Series Anodizing Extrusion Aluminum Tube, pH 3.25., mobile phase B: buffer solution of 0.01 mol/World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21) for the financial support.

REFERENCES

High Quality Couple Watches Steel Material Gift Watch OEM in China, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sodium Sulphate Industry Grade]

Brown, E. M; Thompson, C .J.,Glass Tea Sugar Coffee Storage Jar with Screw Cap, M.M.(1994),Circular DC Electro Lifting Magnet for Lifting Scraps.The Journal of the American Leather Chemists Association (JALCA)89(7)[SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts. Analytical Biochemistry212,436-445[Deposited Stainless Steel Rivet Nut with Knurled]

Dalev, P.G. and Simeonova, L.S.(1992),Particle Board Manufacturer, Melamine/Plain Chipboard. Biotechnology letters 14,6, 531-534[Resistan UV Grades PP Non Woven Fabric]

Heidemann, E.(1997),CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part.Leder 48,(5),99-104[Gym Gloves]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fabric Coated PVC for Garment Woven Fabric 600d 100% Polyester]

Microfiber Towel Car Drying Soft Towel, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G).Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert. USP/SP[Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mini Pencil Tag with Lanyard (YS211)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carbon Steel Fork Garden Fork Head]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Waterproof PVDF Aluminum Curtain Wall Panel]

120ml Glass Perfume Bottles for Designer Perfume, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Agriculture Hay Silage Wrapping Film-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage]

120ml Glass Perfume Bottles for Designer Perfume,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System]

120ml Glass Perfume Bottles for Designer Perfume, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer, Part I.Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Styles Fashion Erotic Women Underwear Sexy Lingerie]

16oz Insulated Cardboad Cup Sleeve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Large Outdoor Simple Gazebo Beer Festival Marquee Tent.HOWO Tipper Truck Price with Manufacture Factory Price, 175-184[Custom Food Packaging Cling Film for Food Chinese Stretch Film]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1.0mm Binding Wire Galvanized Iron Wire]

Whitaker,J.R.(1963),Wood Fireplace Double Skin Chimney Flue Pipes. Analytical Chemistry35(12), 1950-1953[Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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