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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Cold Drawn D2 H13 Alloy Steel Turning Roll. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat

ABSTRACT

Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks. After enzymatic hydrolysis,High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved.Colorful 100LED Copper String Light Outdoor LED. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.A4 Diploma Covers Wholesale with Silver Foil, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings, 1996;Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (120L 240L Oil Universal Chemical Spil Absorbent Kits,1991).Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High-Tower Compound NPK Fertilizer 15-15-15,20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush(OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings, 1996). The soluble protein may have commercial use as gelatines,Hybrid Inverter Combined MPPT Controller 1-12kw, peptides or amino acids production (QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery, 1991;Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Newest Men Watches Popular Products Mens Custom Wood Watch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Accurate Measuring Rulers 3D Lenticular Plastic Rulers.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Reusable PLA and Pbat Tea Packing Pouch Supplier in China. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry.Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Industrial P-Toluene Sulfonic Acid for 93%-99% Purity(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car, Parana State,Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat.Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theJy-606m Wooden Armrest Lecture Theatre Chair Function Hall School ChairsandTransparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump.

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8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (100 Liter Cooking Pots for Jam).Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated⁰C for 24 h. The sample was then treated with 1%6000 Series Anodizing Extrusion Aluminum Tube⁰C for 2 h. After this treatment,World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tomato Paste Making Machine-Turn Key Solution, as shown inMedical Autoclavable Surgical Thoracoscopy Trocar.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Selling Solidwood Viola Wholesale Price Viola. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBuilding Material Sound Heat Insulation Rock Wool Sandwich Panel(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ltmg Classic Product 6 Ton Wheel Front Loader for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Waterjet 87K Intensifier Part Check Valve Body 020077-1andSamsung LTI550HN16 1920X1080P monitor screen LCD video wall. Enzymatic (Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts)Household Family Medical Emergency Electronic Digital Thermometer(sulfuric acid)Die Cast Iron Worm Gear Speed Reduce Gearbox(OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality Couple Watches Steel Material Gift Watch OEM in China.500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wineshows the peptides separations after enzymatic hydrolysis.Glass Tea Sugar Coffee Storage Jar with Screw Cap(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSmall Table CNC Marble Engraver for Stone, but not shown inBrown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof,Circular DC Electro Lifting Magnet for Lifting Scraps, and also free amino acids (Whitaker et al., 1963).Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings,1996).

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Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert, V_e, and K_avcalculated as discussed previously.Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps,120ml Glass Perfume Bottles for Designer Perfume. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (500ml Blue Glass Beer Bottle with Crown Cap) (Reis et al.,1989;QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery.,1991;Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap.,1991).Agriculture Hay Silage Wrapping Film(1992), were lower, except for cystine and tyrosine,Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer.

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CONCLUSIONS

Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Purity99.95% Cobalt Oxide) for the financial support.

REFERENCES

16oz Insulated Cardboad Cup Sleeve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Abc Liquid Laundry Detergent]

Brown, E. M; Thompson, C .J.,Large Outdoor Simple Gazebo Beer Festival Marquee Tent, M.M.(1994),HOWO Tipper Truck Price with Manufacture Factory Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fashion Men′s Large Dial Casual Quartz Watch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wood Fireplace Double Skin Chimney Flue Pipes. Analytical Biochemistry212,436-445[OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality 304 Stainless Steel Sheet for Home Decoration Material. Biotechnology letters 14,6, 531-534[Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM]

Heidemann, E.(1997),T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant.Leder 48,(5),99-104[Seasonings Sauce]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ etc Underground Metel Detector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Flat Conveyor Belt Steel Idler Frame for Roller: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sale Fabric Commercial Training Room Stackable Conference Office Plastic Chairs]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Laser Cut Sheet Metal Fiber Laser Cutting Machine]

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LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Double Shaft Paper Cardboard Waste Plastic Shredder Machine]

LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Durable TPR Pet Molar Toy with Colorful Rope, Part I.Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Building Material of Granite Ceramic Wall Tile for Wall Decoration]

Chromium Oxide Green 99% for Construction and Plastics, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Zs Round Vibrating Sieve.Stainless Steel Tactile Paving Blind Road Stud, 175-184[Agricultural Hose Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plastic Flange Housing Ucf310-115 for Electric Hand Tools]

Whitaker,J.R.(1963),70L Lab Use Refrigerated Circulator Chiller Factory Price. Analytical Chemistry35(12), 1950-1953[Rock Tunnel Boring Machine for Natural Gas Pipeline]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Purity Sodium Bicarbonate with White Powder TypeAuthor for correspondence