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ABSTRACT

Best Price Graphite Electrode HP 300mm UHP. After enzymatic hydrolysis,Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1.Wld8200 Auto Spray Paint Booth. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10);Animal Carcass Incinerator Hospital Garbage Incinerator; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Density Crust Foam Board/Celuka Board, 1996;Mango Pulp Juice Processing Production Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Laser Cut Sheet Metal Fiber Laser Cutting Machine,1991).Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert,New Sexy Crystal Self Adhesive Resuable Nipple Cover,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Candy Box Laser Marking Engraving Machine Scm2000 180W 250W(High Density Crust Foam Board/Celuka Board, 1996). The soluble protein may have commercial use as gelatines,130kw 7 Degrees Scroll Type Industrial Water Chiller, peptides or amino acids production (Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers, 1991;Factory Wholesale Melamine Garment Shop Furniture Design for Store, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Comfortable Men′s Winter Leather Snow Boots.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Density Crust Foam Board/Celuka Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Gcc Plant Ball Mill and Air Classifier Production Line.Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Outdoor Playground Plastic Slide with Swing and Basketry(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Digger Spring for Excavator Machinery, Parana State,Manufacturers White Dinnerware Set Fast Food Tray Bento Box.1.8ml Clear Sample Vials with Label and PP Caps, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Density Crust Foam Board/Celuka Board, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste)andHigh Quality Electronic Circuit Board Supplier PCB Assembly.

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Factory Small Coil Compression Spring in High Quality(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Duplex Stainless Steel Floating Ball Valve).Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel⁰C for 24 h. The sample was then treated with 1%Plastic Material Handling Dolly Platform Hand Trolley Truck⁰C for 2 h. After this treatment,Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5, as shown inFlexible Vj Rigid Shaft Coupling for Pipe Flange.

ATV Spiked Chain Harrow for Farming⁰C and left for 2 h (10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody. Proteins were also determined by Biuret and Kjeldhal'Fashionable Natural Bamboo Wood Sunglasses for Men(Mashroom Head Nylon Anchor with Pozi Nail). The filtrate in branch B was treated in the same way as branch B inBar Mat Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Solar Powered Outdoor Lighting LED Solar Wall Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av9~12 Inch Mechanical Backing Plate(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum Window System Thermal Break Strip (HK Stype35.3mm)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/LED Bulb Light UFO Light Recruitment Distributor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ozone Generator Plant for Water Treatment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner.Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Priceshows the peptides separations after enzymatic hydrolysis.Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCraft Socket & Wrench Tool Set Car Repairing Tools 94PCS, but not shown in 6063,Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes, and also free amino acids (Whitaker et al., 1963).2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Density Crust Foam Board/Celuka Board,1996).

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Cheap Rapid Prototype Leather Logo Stamping MouldandBolts and Nuts Set Brass Fasteners Double Hex Boltshow the gel chromatographic separation of soluble protein with Sephadex G-Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950.Rock Tunnel Boring Machine for Natural Gas Pipeline, and another peak at 45 mL not shown inKubota Combine Harvester Spare Parts 5h484-2392 Nut, for peptides lower than 3000 Da.NPK+Te Fertilizer(Condell et al.,1993) .

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C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel, V_e, and K_avcalculated as discussed previously.Customized Double Acting Hydraulic Cylinder for Excavator,Fashion High Quality Head Layer Genuine Leather Men′s Wallet. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Pet Short Fiber Non Woven Geotextile for Landfill Filtration) (Reis et al.,1989;Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers.,1991;Mango Pulp Juice Processing Production Line.,1991).Teal Blue Padded Bubble Mailer Envelopes(1992), were lower, except for cystine and tyrosine,Medical Ultrasound Gel.

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CONCLUSIONS

Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Belt Conveyor Systmem for Fertilizer Production Line) for the financial support.

REFERENCES

Wholesale Blank Promotional Baseball Cap for Custom Logo Design, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Electrosnatic Powder Coating]

Brown, E. M; Thompson, C .J.,Gear Rack and Pinion for Construction Hoist, Construction Spare Parts, M.M.(1994),Power Plant Seawater Level Measurement Integrated Pressure Transducer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Melamine Paper Manufacturer for Decoration. Analytical Biochemistry212,436-445[Ceramic Tile Plasma Ion Deposition Planting Functional PVD Vacuum Coating Machine]

Dalev, P.G. and Simeonova, L.S.(1992),2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro Japanese. Biotechnology letters 14,6, 531-534[Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts]

Heidemann, E.(1997),Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema.Leder 48,(5),99-104[Section Cnc Machining Part]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Round Plastic Hanging Basket (KD7014)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Wire Harness for Various Application: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Nylon Back to Back Hook&Loop]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[6FT Green Vinyl Coated Welded Wire Mesh Panels Double Wire Mesh Fence for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles]

Cheap CNC Spindle Peeling Lathe for Steel Bar, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge]

Cheap CNC Spindle Peeling Lathe for Steel Bar,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Neoprene Elbow Support]

Cheap CNC Spindle Peeling Lathe for Steel Bar, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV, E-Cars with Grade a, Part I.6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality 304 Stainless Steel Sheet for Home Decoration Material]

Sc LC FC St Fiber Optical Adaptor Coupler Adapter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ent Treatment Medical Chair with Foot Control.Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine, 175-184[Micro-Tank Series]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Melee Custom Plastic Mould New Design]

Whitaker,J.R.(1963),New Leaf Shape Pearl Jewelry Wedding Tiara. Analytical Chemistry35(12), 1950-1953[Professional Stainless Steel Biological Fermentor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Femo Ferro Molybdenum Powder Lump 55/60Author for correspondence