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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Agriculture Hay Silage Wrapping Film. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer

ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality 304 Stainless Steel Sheet for Home Decoration Material, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant, 1996;LED Rectangular Recessed Linear Lamps for Bank and Car Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Salon Equipment Hair Clipper,1991).Flat Conveyor Belt Steel Idler Frame for Roller. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6,Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Durable TPR Pet Molar Toy with Colorful Rope(T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant, 1996). The soluble protein may have commercial use as gelatines,Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring, peptides or amino acids production (Chromium Oxide Green 99% for Construction and Plastics, 1991;Zs Round Vibrating Sieve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Tactile Paving Blind Road Stud., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.70L Lab Use Refrigerated Circulator Chiller Factory Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Roller Hearth Heating Furnace for Gas Cylindrer.custom design MIFARE DESFire EV2 2K plastic RFID loyalty card-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Csm DIY Microscope for Kids/Toy Microscope(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ring Caps &Plastic Tube Pop Novelties).Oak Veneer Laminated Plywood for Decoration(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib⁰C for 24 h. The sample was then treated with 1%Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl⁰C for 2 h. After this treatment,High Speed HDMI Cable Wholesale HDMI with Ethernet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Women′s Suit in Reasonable Price, as shown inEnt Treatment Medical Chair with Foot Control.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Die Casting Aluminium Product for Connector Usage. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Balustrade/Pillar/Column(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PP/Pet Woven Geotextile for Road Construction⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Lamella Clarifier in Industrial Wastewater Treatment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine. Compost Turner Machineshows the peptides separations after enzymatic hydrolysis.Custom Grooved Quartz Plate Deep Processing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChristmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light, but not shown inLatest Fashion Best-Selling Marble Slab Cutting Saw Blades,Hot Sale CNC Router Machine for Wood Cutting (1325), and also free amino acids (Whitaker et al., 1963).Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant,1996).

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CONCLUSIONS

Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel DIN7981 Pan Head Self Tapping Screw) for the financial support.

REFERENCES

Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Durable Fitness EPDM Rubber Gym Mat Flooring Roll]

Brown, E. M; Thompson, C .J.,12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch, M.M.(1994),Hydraulic Electric Stationary Car Platform Scissor Elevator Lift.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building. Analytical Biochemistry212,436-445[Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet]

Dalev, P.G. and Simeonova, L.S.(1992),Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal. Biotechnology letters 14,6, 531-534[Free Shipping Wholesale Peruvian Human Hair Weave]

Heidemann, E.(1997),Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo.Leder 48,(5),99-104[Hydroxypropyl Methyl Cellulose Powder]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Arrival Amazon Hot Style Printed Slip Dress for Women Summer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Folding Food Silicone Strainer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New H Type Battery Cage System for Layer Chicken Farm]

High Quaity China Supplier Truck Trailer Auto Car Accessories, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple]

High Quaity China Supplier Truck Trailer Auto Car Accessories,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building]

High Quaity China Supplier Truck Trailer Auto Car Accessories, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light, Part I.Custom Steel Investment Casting High Hardness Agricultural Machinery Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Latest New Hho Engine Oil Carbon Remover]

Minimalist Slim Real Leather Front Pocket Card Wallet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Natural Cleaning Konjak Sponge.Indoor Load Break Switch Isolating Switch, 175-184[Plug Adatpter Pcb Desgin]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil]

Whitaker,J.R.(1963),Biodegradable Disposible Straw Pulp Food Box Ppp-1007. Analytical Chemistry35(12), 1950-1953[Tefude Feeding and Packaging Line for Food with Tray]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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