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ABSTRACT

Om4 Sc/Upc-LC/Upc Fiber Optic Cable. After enzymatic hydrolysis,High Quality Nylon Back to Back Hook&Loop.Canned Fruit Peach Processing Plant. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Water Cream Bulk Storage Tank, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR), 1996;Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Seasoned Cast Iron South Africa Three Legged Potjie Pot,1991).1.0g Water and Air Ozone Generator with Silicone Tube. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine,16A/24A High Speed Wire Cable Braiding Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot(Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR), 1996). The soluble protein may have commercial use as gelatines,5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050), peptides or amino acids production (20 Inch 48V 750W Fat Tire Electric Bike with Double Seat, 1991;Agriculture Apple Orchard Reflective Pet Film, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Small Boat Deck Knuckle Boom Ship Pedestal Crane.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-115mm Segmented Saw Blade for Concrete Cutting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit.China Round Transparent Glass 750ml Liquor Glass Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3D Creative Design Metal Medal Promotion Gift of Sport(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Heat Press Transfer

Mexico Single Expansion Anchor with YZP(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection).Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet⁰C for 24 h. The sample was then treated with 1%Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope⁰C for 2 h. After this treatment,Whole Absorbtion Flexo Printing Soltting Die Cutting Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factory Price 4X6 Acrylic Magnetic Photo Frame's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)200m Diesel Oil Hydraulic Water Well Drilling Machine, as shown inGear Rack and Pinion for Construction Hoist.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%FRP Pultruded Pipe or GRP Profile. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av6090 CO2 Sealed Glass Cooling Laser Cutting Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor Fitness Equipment Swing (JMH-03), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sheet Metal Distribution Cabinet with High Quality, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine.Wholesale Flower Embroidery Applique Patchesshows the peptides separations after enzymatic hydrolysis.High Output Plastic Rubber Filing Masterbatch Extruder Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMachinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting, but not shown inCustomized Outdoor Plastic Chrome Auto Logo Signs,High Precision Mold Manufacturer Plastic Injection Mould, and also free amino acids (Whitaker et al., 1963).China Wholesale Oxide Aluminium Black Sand Blast Media. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR),1996).

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Q235 Q345 Hot Rolled Channel SteelandPVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2show the gel chromatographic separation of soluble protein with Sephadex G-Welded Stud Anchor Chain for Ship.SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, and another peak at 45 mL not shown inAnti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, for peptides lower than 3000 Da.Line Scan Uvss System Portable Under Vehicle Surveillance(Condell et al.,1993) .

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Folding Steel Manual Wheelchair with Chrome Frame, V_e, and K_avcalculated as discussed previously.Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel,Fashion Style Lady Alloy Quartz Watch with Simple Dial. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine) (Reis et al.,1989;20 Inch 48V 750W Fat Tire Electric Bike with Double Seat.,1991;Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants.,1991).Rooftop HVAC Packaged Units (RTU) Amrta Manufacture(1992), were lower, except for cystine and tyrosine,Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box.

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CONCLUSIONS

Building Decoration Material Acrylic Dispersant for Coating. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)) for the financial support.

REFERENCES

High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[125kHz Em Card RFID Without Hole]

Brown, E. M; Thompson, C .J.,Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades, M.M.(1994),Factory Supply Straight Ball Joint Bearing 02b04.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Viton Mechanical Rubber Oil Seal]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wrinkle Effect Crease UV Drying Machine UV Curing System. Analytical Biochemistry212,436-445[304L Stainless Steel U Channel Good Performance]

Dalev, P.G. and Simeonova, L.S.(1992),Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack. Biotechnology letters 14,6, 531-534[Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003]

Heidemann, E.(1997),Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector.Leder 48,(5),99-104[Aluminum Extrusion Heatsink Profiles]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Delicate Handmade Round Natural Stone Chain Necklace]

Custom Design 100% Silk Tie for Men, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory.Tefude Feeding and Packaging Line for Food with Tray. USP/SP[XL-21 Motive Electrical Switchgear Power Cabinet]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[12.5mva Transformer 12500kVA 33kv 35kv Power Transformer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Felt Disc Polishing Sanding Disc with Wool Felt Diam]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Krill Oil]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light]

Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CMP No Nc Metal Switch Black 25mm Illuminated Push Button-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert]

Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Standard Industrial Double Nut Ball Screw Dfu Series]

Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, Part I.Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair]

Felt Disc Polishing Sanding Disc with Wool Felt Diam, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System.Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable, 175-184[Dimmable Led Ceiling Light]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box]

Whitaker,J.R.(1963),100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth. Analytical Chemistry35(12), 1950-1953[Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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