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ABSTRACT

M Type Metallic Yarn Lurex Yarn Embroidery Yarn. After enzymatic hydrolysis,Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W.Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment;YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Anti-Pollution Modified Acrylic Solid Surface for Countertops, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose, 1996;Corrugated Carton Box Fully Automatic Folding Gluing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (800lb 1/2 A105 Thread Forged Steel Globe Valve,1991).Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal,CNC Machining Metal Mechanical Engineering Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Low Sulphur Calcined Carbon Raiser(Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose, 1996). The soluble protein may have commercial use as gelatines,Hot Selling Free Sample Natural Fragrant Bath SPA Salt, peptides or amino acids production (Custom FSTN Va COB Cog Segment LCD Display Module, 1991;Jc680 High Temperature Pressure Transmitter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Deep Hole Cylinder Boring Machine for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Selling 202 Stainless Steel Coil.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-NPK 16X Hammer Spare Parts Racing Forged Piston. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve.Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mobile Hydraulic Concrete Placing Boom(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Kt Board Acrylic CCD Camera CNC Knife Cutting Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic).High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced⁰C for 24 h. The sample was then treated with 1%Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks⁰C for 2 h. After this treatment,Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box, as shown inLow Power Consumption Silicone Sex Doll Vending Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Bend Pipe for Industrial Use, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ybz-E16f20 Vane Steering Hydraulic Pumps, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

CMC Transparent Grade for Coating ProductionandGood Quality Snow White Pumpkin Seeds for Sale. Enzymatic (ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors)Automotive Acrylic Wholesale Masking Tape Factory Price(sulfuric acid)Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price(Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, flow rate of 0.1 mL/min., and l = 620 nm.

Watermrk Drop in Bathroom Basin Sink.Stainless Steel Tactile Paving Blind Road Studshows the peptides separations after enzymatic hydrolysis.OEM ABS Industrial Plastic Sheet Injection Auto Part(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMango Pulp Juice Processing Production Line, but not shown inSS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving,15L Big Cooling Cylinder High Production Hard Ice Cream Machine, and also free amino acids (Whitaker et al., 1963).Flange Type Ball Float Water Tank Control Valve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose,1996).

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CONCLUSIONS

High Quality Beech Wood Chiavari Chair at a Competitive. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cr1000 Spray Nozzle Common Rail Injector Tester) for the financial support.

REFERENCES

Gradient Print Overlay Push Button Membrane Keypad Switch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Chinese Printer Spare Parts for Epson Dx5 /5113 Machines]

Brown, E. M; Thompson, C .J.,Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X, M.M.(1994),Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004).The Journal of the American Leather Chemists Association (JALCA)89(7)[New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform. Analytical Biochemistry212,436-445[Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp]

Dalev, P.G. and Simeonova, L.S.(1992),55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk. Biotechnology letters 14,6, 531-534[100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth]

Heidemann, E.(1997),AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle.Leder 48,(5),99-104[Alarm Perimeter Beam Detector with 2 Beams]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Low Volume Hard Tool Kit Thermoplastic Polymer Injection Molding]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Flange Type Ball Float Water Tank Control Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cheap Galvanized Iron Wire]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Men V-Neck Longline T-Shirt with Color Block Ribbed Trims]

63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Round Airless Lotion Pump Bottles for Skin Care Products-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera]

63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes]

63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Round Shape Dinner Football Gift Bowl with Cover Plate, Part I.Professional Exporter of Wheat Flour Mill Plant (80t). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)]

ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine.Aluminum Alloy Rack for Conductor, Wire, Cable, 175-184[Sim Card Connector To Usb]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage]

Whitaker,J.R.(1963),Outdoor Accessories Fishing Hunting Masks. Analytical Chemistry35(12), 1950-1953[Customized Double Acting Hydraulic Cylinder for Excavator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Professional Manufacturer Stainless Steel Floor GratingAuthor for correspondence