ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Mining Machinery Parts Diamond Saw Blade Segments for Marble. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface

ABSTRACT

Multi Column Load Cell 100kg, 20t. After enzymatic hydrolysis,LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System.Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Foldable PP Plastic Storage Logistics Turnover Box;China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Buddhist Mood Picture Wallpaper Murals, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Flange Dismantling Joint, 1996;Duplex Stainless Steel Floating Ball Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card,1991).Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple,Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CNC Machine Aluminum Telescopic Covers Flat Bellows Cover(High Quality Flange Dismantling Joint, 1996). The soluble protein may have commercial use as gelatines,7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input, peptides or amino acids production (Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence, 1991;Kubota Combine Harvester Spare Parts 5h484-2392 Nut, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Steel Floor Deck Post Cutting Cold Roll Forming Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Flange Dismantling Joint, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180).Fluorous Rubber Coated Fiberglass Cloth-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Custom Free Logo Design Cheap Hand Flag (NF01F02021)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1200mm Recessed Mounted LED Linear Light).125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X⁰C for 24 h. The sample was then treated with 1%RJ45 Cat5e in-Line Coupler Modular Jack⁰C for 2 h. After this treatment,Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052, as shown inMie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use.

High Quality Disposable Blood Transfusion Set with Filter⁰C and left for 2 h (High Quality Crusher for Used Plastic Recycling Line). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194). Proteins were also determined by Biuret and Kjeldhal'High Quality Brushless DC Electric Motor for Industrial Deduster(Outdoor Accessories Fishing Hunting Masks). The filtrate in branch B was treated in the same way as branch B inFolding Automatic Tissue Napkin Paper Making Machine Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Factory Light Soda Ash /Sodium Carbonate.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Regular Size Cigarette Rolling Papers. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av304L Stainless Steel U Channel Good Performance(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Junior Backyard Training Practise Soccer Goal Net⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/350mm Diamond Blade for Granite, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Welding Stainless Steel Sheet Metal Welding Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ss316 Stainless Steel Heavy Duty Straps.Low Sulphur Calcined Carbon Raisershows the peptides separations after enzymatic hydrolysis.6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in12V 24V 220V Digital Temperature Controller, but not shown in(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine,High Temperature Side Opening AAC Autoclave, AAC Block Making Machine, and also free amino acids (Whitaker et al., 1963).PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Flange Dismantling Joint,1996).

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CONCLUSIONS

Furniture Grade E1 Glue Plywood with Competitive Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (K Helical Bevel Gearboxes Reducer Motor) for the financial support.

REFERENCES

High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Estradiol Test Kit Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,TUV Certificate High Quality Single Core PV1-F PV Solar Cables, M.M.(1994),Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners.The Journal of the American Leather Chemists Association (JALCA)89(7)[6090 CO2 Sealed Glass Cooling Laser Cutting Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cast Steel Dual Plate Wafer Check Valve. Analytical Biochemistry212,436-445[5.5X19mm Hex Head Self Tapping Screws with Washer]

Dalev, P.G. and Simeonova, L.S.(1992),Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products. Biotechnology letters 14,6, 531-534[Professional Customization Screen Printing Machine for Porous Ceramic]

Heidemann, E.(1997),Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts.Leder 48,(5),99-104[Delphi Common Rail Injector Control Valve 28239294 9308-621C]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quaity China Supplier Truck Trailer Auto Car Accessories]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Professional Exporter of Wheat Flour Mill Plant (80t)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CATV Indoor Optical Receiver FTTH: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ce Quality Double Channel Medical Syringe Pump (THR-SP180D)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Brick Block Making Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter]

Best Sell 16L Square Dustbin Frame Mould, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container]

Best Sell 16L Square Dustbin Frame Mould,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory]

Best Sell 16L Square Dustbin Frame Mould, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush, Part I.Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wood Chips Rotary Dryer Sawdust Pellet Drying Machine]

PVDF Exterior Design Curved Aluminum Panel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Neodymium Magnet with RoHS ISO 16499 Approved.Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick, 175-184[Walking Assistant Chair Help Walking]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence]

Whitaker,J.R.(1963),Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price. Analytical Chemistry35(12), 1950-1953[High Quality Beech Wood Chiavari Chair at a Competitive]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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