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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Flanged Braided Metal Hose Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Timber Pressure Vessel for Wood Processing, 1996;1200mm Recessed Mounted LED Linear Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (The Big Diameter Carbide Tipped Hole Saw,1991).Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale,Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion Men′s Large Dial Casual Quartz Watch(Timber Pressure Vessel for Wood Processing, 1996). The soluble protein may have commercial use as gelatines,PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence, peptides or amino acids production (Outdoor Garden Furniture Baby Plastic Swing for Sale, 1991;Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2D LED Rope Lighting Motif Lights for Christmas Decoration., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Modern Compact Laminate Key Lock Changing Room Locker.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor Full Color LED Video Wall. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Timber Pressure Vessel for Wood Processing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125).Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ampoule Sealer Machine Hydrogen Oxygen Gas Generator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sheet Metal Distribution Cabinet with High Quality).Commercial Double Lane Inflatable Carnival Bungee Run Game(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair⁰C for 24 h. The sample was then treated with 1%Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale⁰C for 2 h. After this treatment,800mm Length 600n Load Tension Gas Spring with Metal Eyelet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers, as shown inHigh Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4.

Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant⁰C and left for 2 h (99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale. Proteins were also determined by Biuret and Kjeldhal'100 Liter Cooking Pots for Jam(Artnet Node Artnet to DMX Controller). The filtrate in branch B was treated in the same way as branch B inHigh Precision Punching Parts Progressive Sheet Metal Die Stamping. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Food Grade Customized Metal Candy Can Square Mint Tin Box.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%600X600 mm Ugr 19 LED Panel Lights. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGood Quality Hot Tube8 Japanese Stainless Steel Tube 666(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Round Plastic Hanging Basket (KD7014)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Muliti-Size Light Hot Stamping Foil for PS Frame. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine) for the financial support.

REFERENCES

Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Lifting Chain]

Brown, E. M; Thompson, C .J.,50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court, M.M.(1994),China 1 & 100 RMB Acrylic Currence Frame.The Journal of the American Leather Chemists Association (JALCA)89(7)[16A/24A High Speed Wire Cable Braiding Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Toughened Glass Disc Suspension Insulator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-1 Ton Toyo Type Manual Chain Block. Analytical Biochemistry212,436-445[Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Spark Resistant Construction Axial Fan with Fixed Pitch Blade. Biotechnology letters 14,6, 531-534[Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Precision CNC Milling DIN69871. Mas403 for CNC Machinist: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot-DIP Galvanized Steel Outdoor Flood Light Solar LED Street Lamp]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Round Hole Carbon Steel Pipe Perforated for Filter]

Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Building Material of Granite Ceramic Wall Tile for Wall Decoration-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fashionable Natural Bamboo Wood Sunglasses for Men]

Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial]

Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine, Part I.Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)]

Whitaker,J.R.(1963),Metal Container Packaging for Edible Oil Tin Cans Storage Boxes. Analytical Chemistry35(12), 1950-1953[Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Dentist Used Metal Dental Steel Nickel Chrome with Beryllium AlloyAuthor for correspondence