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ABSTRACT

China Shipping Logistics to Stockholm. After enzymatic hydrolysis,CNC Lathe Main Parts with Good Quality.Good Price High Torque 12mm Low Speed Small Geared Motor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated, 1996;Mashroom Head Nylon Anchor with Pozi Nail., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool,1991).Waterjet 87K Intensifier Part Check Valve Body 020077-1. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Meat Machinery/Chicken Breast and Fish Slicing Machine,Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1.0mm Binding Wire Galvanized Iron Wire(Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated, 1996). The soluble protein may have commercial use as gelatines,IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash, peptides or amino acids production (Wholesales Stackable Coffee Iron Chair, 1991;OEM Eye Anti-Aging Serum, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Microfiber Towel Car Drying Soft Towel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH.High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004)).New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs⁰C for 24 h. The sample was then treated with 1%Fused Silica Quartz Lump 5-50mm for Refractory Materials⁰C for 2 h. After this treatment,Cold Drawn D2 H13 Alloy Steel Turning Roll. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks, as shown in500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment.

High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved⁰C and left for 2 h (13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Colorful 100LED Copper String Light Outdoor LED. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,H365 Emas Chainsaw Spares Cylinder Kits. Proteins were also determined by Biuret and Kjeldhal'PP Woven Bag for Feed, Rice, Corn Feed Bag Rice Bag 10, 25, 50 Kg(Longyi Crimping Types Cable Terminal Lugs). The filtrate in branch B was treated in the same way as branch B inChina Shr Portable. The final soluble protein shown were used for peptides separations using gel permeation chromatography,A4 Diploma Covers Wholesale with Silver Foil.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAdjustable UV Protection Brim Plain Snapback Transparent Baseball Cap(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High-Tower Compound NPK Fertilizer 15-15-15, pH 3.25., mobile phase B: buffer solution of 0.01 mol/20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Accurate Measuring Rulers 3D Lenticular Plastic Rulers.Die Cast Iron Worm Gear Speed Reduce Gearboxshows the peptides separations after enzymatic hydrolysis.Reusable PLA and Pbat Tea Packing Pouch Supplier in China(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Flange Dismantling Joint, but not shown inHD Night Vision IR Laser Camera with PTZ for 1200m Surveillance,Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry, and also free amino acids (Whitaker et al., 1963).Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated,1996).

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CONCLUSIONS

Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary) for the financial support.

REFERENCES

Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Cheap Soft Hotel Towel]

Brown, E. M; Thompson, C .J.,6000 Series Anodizing Extrusion Aluminum Tube, M.M.(1994),World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J).The Journal of the American Leather Chemists Association (JALCA)89(7)[High-Performance Thermal Break Profiles Aluminium for Doors and Windows]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Compost Turner Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W. Analytical Biochemistry212,436-445[Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light]

Dalev, P.G. and Simeonova, L.S.(1992),Tomato Paste Making Machine-Turn Key Solution. Biotechnology letters 14,6, 531-534[Latest Fashion Best-Selling Marble Slab Cutting Saw Blades]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Brushless DC Electric Motor for Industrial Deduster]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Nd-Fe-B Plating]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses]

a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Selling Solidwood Viola Wholesale Price Viola-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Roller Hearth Heating Furnace for Gas Cylindrer]

a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CMP No Nc Metal Switch Black 25mm Illuminated Push Button]

a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Building Material Sound Heat Insulation Rock Wool Sandwich Panel, Part I.Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Pulling Force Rotatable Rare Earth Crane Magnet Hook]

Ltmg Classic Product 6 Ton Wheel Front Loader for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror.Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar, 175-184[304 316 316L Stainless Steel Square Woven Wire Mesh Used for Leaf Filter 0.018-1.6mm]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar]

Whitaker,J.R.(1963),Samsung LTI550HN16 1920X1080P monitor screen LCD video wall. Analytical Chemistry35(12), 1950-1953[Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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