ARTICLES

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ABSTRACT

Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine. After enzymatic hydrolysis,Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa.Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Hot Rolled Steel U Channel China Supplier;M Type Metallic Yarn Lurex Yarn Embroidery Yarn; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck, 1996;500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable,1991).Anti-Pollution Modified Acrylic Solid Surface for Countertops. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose,Corrugated Carton Box Fully Automatic Folding Gluing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,800lb 1/2 A105 Thread Forged Steel Globe Valve(Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck, 1996). The soluble protein may have commercial use as gelatines,Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, peptides or amino acids production (China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal, 1991;CNC Machining Metal Mechanical Engineering Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Low Sulphur Calcined Carbon Raiser., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Selling Free Sample Natural Fragrant Bath SPA Salt.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom FSTN Va COB Cog Segment LCD Display Module. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Jc680 High Temperature Pressure Transmitter.Deep Hole Cylinder Boring Machine for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Selling 202 Stainless Steel Coil(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cr1000 Spray Nozzle Common Rail Injector Tester(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (4 Inch Quarter Round PCD Diamond Grinding Cup Wheel).Industrial Cast Steel Flanged One Way Swing Check Valve Nrv(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Black White Stone 925 Silver Cross Pendants Necklace Jewelry⁰C for 24 h. The sample was then treated with 1%Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People⁰C for 2 h. After this treatment,Kt Board Acrylic CCD Camera CNC Knife Cutting Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced, as shown inCustomized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging.

Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks⁰C and left for 2 h (High Tensisle Strength Tissue Paper). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part. Proteins were also determined by Biuret and Kjeldhal'Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box(Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl). The filtrate in branch B was treated in the same way as branch B inBraided Hose Crimpring Machine Wholesaler Quotes & PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCarbon Steel Stainless Steel Countersunk Head Hollow Bolt(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable, pH 3.25., mobile phase B: buffer solution of 0.01 mol/5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors.Big Bussiness Project Commerical Chicken Poultry Farm Buildingsshows the peptides separations after enzymatic hydrolysis.Automotive Acrylic Wholesale Masking Tape Factory Price(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card, but not shown inStone PVC Luxury Vinyl Plank Spc Flooring with V Groove,Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price, and also free amino acids (Whitaker et al., 1963).Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck,1996).

Vegetation Protein

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Flange Type Ball Float Water Tank Control Valve, V_e, and K_avcalculated as discussed previously.925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring,Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (HPMC Hydroxy Propyl Methyl Cellulose Additives) (Reis et al.,1989;China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal.,1991;500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment.,1991).Customized Outdoor Plastic Chrome Auto Logo Signs(1992), were lower, except for cystine and tyrosine,Small Table CNC Marble Engraver for Stone.

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CONCLUSIONS

High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector) for the financial support.

REFERENCES

China Soundproof Construction Foam PVC/WPC Board, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Jig And Fixture Parts]

Brown, E. M; Thompson, C .J.,UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould, M.M.(1994),High Pressure Braided Rubber Oil Hose.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Beech Wood Chiavari Chair at a Competitive. Analytical Biochemistry212,436-445[Metric Female Cone Hydraulic Hose Fitting]

Dalev, P.G. and Simeonova, L.S.(1992),Gradient Print Overlay Push Button Membrane Keypad Switch. Biotechnology letters 14,6, 531-534[Green Color PVC Anti Dazzle Panel]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Accelerometer Sensor Price Products Made in China]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Full Color Indoor Outdoor LED Curtain TV Display]

AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Low Power Consumption Silicone Sex Doll Vending Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hybrid Inverter Combined MPPT Controller 1-12kw]

AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dual Plastic 9 Volt Battery Clips Connector with Wire]

AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Toughened Glass Disc Suspension Insulator, Part I.Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules]

63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized Round Airless Lotion Pump Bottles for Skin Care Products.Round Shape Dinner Football Gift Bowl with Cover Plate, 175-184[Bicycle Derailleur Pulley]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fashion Women Hair Accessories Hair Bow Headband for Girls]

Whitaker,J.R.(1963),Professional Exporter of Wheat Flour Mill Plant (80t). Analytical Chemistry35(12), 1950-1953[E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating MachineAuthor for correspondence