ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Permanent Suspended Magnetic Separator for Belt Conveyor. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Double Sides Clear White Frosted Acrylic Sheet

ABSTRACT

5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery. After enzymatic hydrolysis,20W Solar Street Light with Lens Motion Sensor 2 Years Warranty.CNC Machined Power Transmission Shaft Part. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell;Good Price Channel Steel Used for Machinery; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, 1996;Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Direct Sale Patent up to Down Rubber Tile Curing Press,1991).Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Real Leather Wallet Mens Designer Leather Wallets,Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Full Port Small Automatic Electronic Plumbing Ball Valve(Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, 1996). The soluble protein may have commercial use as gelatines,Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,, peptides or amino acids production (Cleanroom White PVC Dotted Anti-Static Gloves, 1991;High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Marine Solas Adhesive Reflective Tape., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰ASTM A240 304 316L No. 1 Stainless Steel Sheet.12V 24V 220V Digital Temperature Controller-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed, Parana State,Double Sides Clear White Frosted Acrylic Sheet.Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAustralia Standard Structural Steel of Steel Structure Hangar As4100 Airport BuildingandLatest New Hho Engine Oil Carbon Remover.

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Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Tefude Feeding and Packaging Line for Food with Tray).Rpl Straight Socket and Plugs Male Thread(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall⁰C for 24 h. The sample was then treated with 1%OEM Metal Stamping Stainless Steel Parts with CNC Machining⁰C for 2 h. After this treatment,Ss Wafer Type Check Valve Dn32 Dn40 Dn50. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape, as shown in24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum.

8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera⁰C and left for 2 h (Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.WPC Wood Plastic Board Equipment Extruder Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS. Proteins were also determined by Biuret and Kjeldhal'Neoprene Elbow Support(2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush). The filtrate in branch B was treated in the same way as branch B inHd Security Camera Cable. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Produce Medical Machining Custom Metal Milling. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMulti Sensor Nilitary Surveillance Thermal Imaging Security camera(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation, pH 3.25., mobile phase B: buffer solution of 0.01 mol/275ml Frosted Clear Glass Drinking Bottle for Juice, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Good Quality Hot Tube8 Japanese Stainless Steel Tube 666andEPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price. Enzymatic (Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs)Bolts and Nuts Set Brass Fasteners Double Hex Bolt(sulfuric acid)Factory Direct Sales Kids Boy Cotton Children T Shirt(Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener, flow rate of 0.1 mL/min., and l = 620 nm.

15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi.Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machineshows the peptides separations after enzymatic hydrolysis.Light Blue PVC Plastic Sheet and PVC Film(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLow Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable, but not shown inFactory Sale Various Customized Square 6063 Extruded Aluminum Profile,Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy, and also free amino acids (Whitaker et al., 1963).Metric Female Cone Hydraulic Hose Fitting. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology,1996).

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Double Component Js Polymer Cement Waterproof Coating for Kitchen BathroomandLED Rectangular Recessed Linear Lamps for Bank and Car Saleshow the gel chromatographic separation of soluble protein with Sephadex G-Hot Sale Dental Material Dental PMMA Block From China.Plastic Waste Pet Flakes Recycling Washing Machine, and another peak at 45 mL not shown inEco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine, for peptides lower than 3000 Da.Deposited Stainless Steel Rivet Nut with Knurled(Condell et al.,1993) .

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High Quality Stainless Steel Mechanical Filter Hosing, V_e, and K_avcalculated as discussed previously.Paper New Year Greeting Cards Wedding Invitations Cards,Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools) (Reis et al.,1989;Cleanroom White PVC Dotted Anti-Static Gloves.,1991;Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount.,1991).BLE5.0 Ttl to Bluetooth Module with at Command(1992), were lower, except for cystine and tyrosine,Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle.

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CONCLUSIONS

Professional Customization Screen Printing Machine for Porous Ceramic. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rotor High Output Electric 120 Watt Motor) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving, M.M.(1994),Square Shape Glass Candle Holder for One Piece Tealight Candle.The Journal of the American Leather Chemists Association (JALCA)89(7)[AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box. Analytical Biochemistry212,436-445[Stainless Steel Water Cream Bulk Storage Tank]

Dalev, P.G. and Simeonova, L.S.(1992),Medical Autoclavable Surgical Thoracoscopy Trocar. Biotechnology letters 14,6, 531-534[Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building]

Heidemann, E.(1997),DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge.Leder 48,(5),99-104[OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mexico Single Expansion Anchor with YZP]

Plastic Packaging Tray Vacuum Forming Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Easy Foldable Kids Walking Chair Toys Baby Walker.Men V-Neck Longline T-Shirt with Color Block Ribbed Trims. USP/SP[N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Welded Stud Anchor Chain for Ship]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stone Carving Flower Pot for Garden Decoration]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mini Ice Maker Machine Cube with Ce / ISO/ RoHS Factory Supply]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Animal Carcass Incinerator Hospital Garbage Incinerator]

Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor Multifunctional Solar Power Flashlight (SYSG-517)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer]

Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale]

Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automobile Parts Chimney Fiberglass, High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product, Part I.Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine]

High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),FC Bare Fiber Optic Adapter for Network.3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory, 175-184[Bimetal Strip]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights]

Whitaker,J.R.(1963),Cattle Corpse Cremation Burning Stove. Analytical Chemistry35(12), 1950-1953[ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

A5 PU Cover Loose Leaf Business Diary Book Notepad NotebookAuthor for correspondence