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ABSTRACT

Natural Birch F&B Veneer Furniture Plywood. After enzymatic hydrolysis,Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator.High Quality Tire Repair Tool Kit, Tire Repair Strip. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Performance 915MHz PCB Antenna;GRP/FRP Profile Structures/Fiberglass Pultrusion Product; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering, 1996;Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Metal Material Products for Punching Stamping Part,1991).Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller,Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place(High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering, 1996). The soluble protein may have commercial use as gelatines,Theme Park Inflatable Bouncer for Sale (BJ-B16), peptides or amino acids production (Muliti-Size Light Hot Stamping Foil for PS Frame, 1991;Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China 1 & 100 RMB Acrylic Currence Frame.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1 Ton Toyo Type Manual Chain Block. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Spark Resistant Construction Axial Fan with Fixed Pitch Blade.Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (A4 Diploma Covers Wholesale with Silver Foil).Precision Customized CNC Machined Mild Steel Flange for Automobile(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing⁰C for 24 h. The sample was then treated with 1%Metal Container Packaging for Edible Oil Tin Cans Storage Boxes⁰C for 2 h. After this treatment,Melee Custom Plastic Mould New Design. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Copper-Pressed V-Type Expansion Pipe Joint, as shown inFudan Compatible Card 1k RFID Card 13.56MHz.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAmbarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Flower Embroidery Applique Patches, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Big Bussiness Project Commerical Chicken Poultry Farm Buildings, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality Crusher for Used Plastic Recycling Line.Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Choppershows the peptides separations after enzymatic hydrolysis.300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inUSB LED Clock Fan USB-Powered Portable Fan with Clock, but not shown inChinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004),Ceramic Bearing Si3n4 608, and also free amino acids (Whitaker et al., 1963).Nbm High Quality Auto Part Factory Price 6pk2310. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering,1996).

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CONCLUSIONS

Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect) for the financial support.

REFERENCES

Electric Heating Industrial Jacketed Glass Reactor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[8 in 1 3.1 USB C Type C Hub with 4K HDMI Adapter for MacBook PRO]

Brown, E. M; Thompson, C .J.,40X-1600X Biological Microscope Xsz-107V LED Light, M.M.(1994),6063 Aluminum Coil for Commercial Vehicle Floor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Two Sided Lighted Vanity Mirror Folding 1X and 5X or 10X LED Makeup Mirrors]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Silicon Carbide Foundry Porous Ceramic Foam Filter. Analytical Biochemistry212,436-445[High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124]

Dalev, P.G. and Simeonova, L.S.(1992),1200X1000X810 Strong Collapsible Plastic Containers for Storage. Biotechnology letters 14,6, 531-534[Ring Caps &Plastic Tube Pop Novelties]

Heidemann, E.(1997),Steel Construction in Poultry House with Good Quality and Installation.Leder 48,(5),99-104[3/5/7 Ply Cardboard Corrugator Line]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ent Treatment Medical Chair with Foot Control]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Direct Sales Kids Boy Cotton Children T Shirt]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stone Cracking Rock Cutting Cement: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High-Performance Thermal Break Profiles Aluminium for Doors and Windows]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pressure Infusion Cuff Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Compost Turner Machine]

8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light]

8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Latest Fashion Best-Selling Marble Slab Cutting Saw Blades]

8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator, Part I.PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller]

Aluminum Hot Forging Accessories Machining Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Building Wall Machine for Hot Machine.New Arrival Amazon Hot Style Printed Slip Dress for Women Summer, 175-184[Outer Surface Chromium Piston Ring]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Iron Wire Mesh Chain Link Mesh Security Fence]

Whitaker,J.R.(1963),Metal Cuting CNC Plasma Cutting Machine (DW1325). Analytical Chemistry35(12), 1950-1953[High Quality Brushless DC Electric Motor for Industrial Deduster]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment EquipmentAuthor for correspondence