ARTICLES

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ABSTRACT

Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil. After enzymatic hydrolysis,Felt Disc Polishing Sanding Disc with Wool Felt Diam.13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable;100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.240T goodeng water/oil/gas pipe drilling rig HDD machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas, 1996;Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen,1991).Colorful Braided Fabric Cable Round. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Universal Wholesale Hot Sale Custom Blank Keys,Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30(Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas, 1996). The soluble protein may have commercial use as gelatines,Waw 8 Ton Logistics Light Truck for Transportation, peptides or amino acids production (Hot Forging Lathe Spindle Forged Spindle, 1991;Hot Sale Chic Straw Braid Floppy Hat with Print Tape, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lamella Clarifier in Sewage Tretament Plant for Water Purification.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-BMC (DMC) / SMC Compression Moulding. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ring Caps &Plastic Tube Pop Novelties.Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada, Parana State,Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks.Customized Stainless Steel Laser Cutting, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBiobase China Vertical Type-Double Doors -25º C Freezer for Best PriceandDigital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter.

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Price of a Retail Supermarket Push Cart for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( SLA Shoes Prototyping for Auto Accessory).Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate⁰C for 24 h. The sample was then treated with 1%Handmade Natural Bamboo Basket / Bamboo Flower Pot⁰C for 2 h. After this treatment,Stainless Steel Handrail Bar Holder for 16mm Pipe. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Shac Flange Linear Bearing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price, as shown inPressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp.

Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape⁰C and left for 2 h (Sludge Oil Separation Centrifuge). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CNC Router for Engraving Cutting Carving Drilling Milling. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine. Proteins were also determined by Biuret and Kjeldhal'Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa(Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post). The filtrate in branch B was treated in the same way as branch B inPaper Product Making Machinery. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Hot Rolled Steel U Channel China Supplier. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avM Type Metallic Yarn Lurex Yarn Embroidery Yarn(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Real Leather Wallet Mens Designer Leather WalletsandAnti-Pollution Modified Acrylic Solid Surface for Countertops. Enzymatic (Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose)Corrugated Carton Box Fully Automatic Folding Gluing Machine(sulfuric acid)800lb 1/2 A105 Thread Forged Steel Globe Valve(Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, flow rate of 0.1 mL/min., and l = 620 nm.

China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal.OEM Branded Low Price Disposable Baby Pull up Diapers in Bulkshows the peptides separations after enzymatic hydrolysis.CNC Machining Metal Mechanical Engineering Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in 60 Hz, but not shown inStone Cracking Rock Cutting Cement,Low Sulphur Calcined Carbon Raiser, and also free amino acids (Whitaker et al., 1963).Hot Selling Free Sample Natural Fragrant Bath SPA Salt. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas,1996).

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CONCLUSIONS

Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection) for the financial support.

REFERENCES

Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[500W~3300W Fiber Laser Cutting of Sheet/Pipe/Tube Cutting Machine]

Brown, E. M; Thompson, C .J.,Cr1000 Spray Nozzle Common Rail Injector Tester, M.M.(1994),Industrial Cast Steel Flanged One Way Swing Check Valve Nrv.The Journal of the American Leather Chemists Association (JALCA)89(7)[TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Painting Road Marking Vehicles Machine for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Black White Stone 925 Silver Cross Pendants Necklace Jewelry. Analytical Biochemistry212,436-445[ Wire]

Dalev, P.G. and Simeonova, L.S.(1992),Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People. Biotechnology letters 14,6, 531-534[Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts]

Heidemann, E.(1997),Kt Board Acrylic CCD Camera CNC Knife Cutting Machine.Leder 48,(5),99-104[Hamster Rat Cage]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel]

Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17.Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced. USP/SP[Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Coffee Machine with Built-in Grinder - APP Controlled - Beans or Pre-Ground]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable]

Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ceramic Bearing Si3n4 608]

Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute]

Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box, Part I.Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Supply Coaxial Cable Rg 59 with Messenger]

Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Carbon Steel Stainless Steel Countersunk Head Hollow Bolt.Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses, 175-184[China Bidirectional Gear Pump]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle]

Whitaker,J.R.(1963),China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture. Analytical Chemistry35(12), 1950-1953[350mm Diamond Blade for Granite]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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