ARTICLES

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ABSTRACT

Stainless Steel Bend Pipe for Industrial Use. After enzymatic hydrolysis,Ybz-E16f20 Vane Steering Hydraulic Pumps.Good Quality Snow White Pumpkin Seeds for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, 1996;Watermrk Drop in Bathroom Basin Sink., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM ABS Industrial Plastic Sheet Injection Auto Part,1991).15L Big Cooling Cylinder High Production Hard Ice Cream Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Flange Type Ball Float Water Tank Control Valve,925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear(Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, 1996). The soluble protein may have commercial use as gelatines,Customized Outdoor Plastic Chrome Auto Logo Signs, peptides or amino acids production (Small Table CNC Marble Engraver for Stone, 1991;High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Soundproof Construction Foam PVC/WPC Board., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Pressure Braided Rubber Oil Hose. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Beech Wood Chiavari Chair at a Competitive.Gradient Print Overlay Push Button Membrane Keypad Switch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Low Power Consumption Silicone Sex Doll Vending Machine, Parana State,High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511).Toughened Glass Disc Suspension Insulator, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIP65 Outdoor Solar Flood Lamp with Battery for CommercialandCNC Metal Grinding Machine Tool Mk2120.

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Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (6090 CO2 Sealed Glass Cooling Laser Cutting Machine).63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Round Airless Lotion Pump Bottles for Skin Care Products⁰C for 24 h. The sample was then treated with 1%Round Shape Dinner Football Gift Bowl with Cover Plate⁰C for 2 h. After this treatment,Professional Exporter of Wheat Flour Mill Plant (80t). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine, as shown inElastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat.

Aluminum Alloy Rack for Conductor, Wire, Cable⁰C and left for 2 h (5.5X19mm Hex Head Self Tapping Screws with Washer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Outdoor Accessories Fishing Hunting Masks. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box. Proteins were also determined by Biuret and Kjeldhal'KERONG Security Safe Combination Smart Key Card Cabinet Door Lock(Professional Customization Screen Printing Machine for Porous Ceramic). The filtrate in branch B was treated in the same way as branch B inChina Small Rf Isolator. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cast Floating Ball Valve China Manufacturer (GAQ41F).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Main Pipe Tee Joint for Railway Piping System(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Promotion Snack Children School Lunch Bag Cooler Bag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Full Automatic Paper Lunch Food Box Carton Forming Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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No 55 Steel Wire Cross Type Razor Barbed Wire, V_e, and K_avcalculated as discussed previously.4V330-10 Air Compressor Solenoid Industrial Valve,Professional Various Standard Hot Sale Rotary Lab Single Round Punch. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Beech Wood Chiavari Chair at a Competitive) (Reis et al.,1989;Small Table CNC Marble Engraver for Stone.,1991;Watermrk Drop in Bathroom Basin Sink.,1991).Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute(1992), were lower, except for cystine and tyrosine,Light Blue Color Glass Drink Bottle for Beer.

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CONCLUSIONS

Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk) for the financial support.

REFERENCES

IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit]

Brown, E. M; Thompson, C .J.,Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, M.M.(1994),Waterproof PVDF Aluminum Curtain Wall Panel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Teflon Seal/Seals Spring Energized]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas. Analytical Biochemistry212,436-445[Stainless Steel Bend Pipe for Industrial Use]

Dalev, P.G. and Simeonova, L.S.(1992),Round Modern Furniture Leisure Sofa for Promotion. Biotechnology letters 14,6, 531-534[Ss Wafer Type Check Valve Dn32 Dn40 Dn50]

Heidemann, E.(1997),Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket.Leder 48,(5),99-104[China Personalized Makeup Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Design High Quality Alminum Sandwich Panel High Pressure Laminate]

5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sany Hg3333m Climbing Placing Boom.Plastic Packaging Tray Vacuum Forming Machine. USP/SP[99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Durable TPR Pet Molar Toy with Colorful Rope]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Permanent Suspended Magnetic Separator for Belt Conveyor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cmc For Shampoo Factory & Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Agriculture Hay Silage Wrapping Film]

Double Sides Clear White Frosted Acrylic Sheet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4)]

Double Sides Clear White Frosted Acrylic Sheet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine]

Double Sides Clear White Frosted Acrylic Sheet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:20W Solar Street Light with Lens Motion Sensor 2 Years Warranty, Part I.CNC Machined Power Transmission Shaft Part. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Machinery Parts Aeroplane Engine CNC Milling]

Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Price Channel Steel Used for Machinery.Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator, 175-184[Construction Building Pipe Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Direct Sale Patent up to Down Rubber Tile Curing Press]

Whitaker,J.R.(1963),Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology. Analytical Chemistry35(12), 1950-1953[Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Steel Pipe Rust Removal Shot Blasting Cleaning MachineAuthor for correspondence