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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Sale Various Customized Square 6063 Extruded Aluminum Profile, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Design 100% Silk Tie for Men, 1996;Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover,1991).Om4 Sc/Upc-LC/Upc Fiber Optic Cable. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Nylon Back to Back Hook&Loop,Canned Fruit Peach Processing Plant,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate(Custom Design 100% Silk Tie for Men, 1996). The soluble protein may have commercial use as gelatines,Garden Furniture Sofa Rattan Modular Corner Set with Cushions, peptides or amino acids production (Stainless Steel Water Cream Bulk Storage Tank, 1991;Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Seasoned Cast Iron South Africa Three Legged Potjie Pot.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1.0g Water and Air Ozone Generator with Silicone Tube. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Design 100% Silk Tie for Men, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine.16A/24A High Speed Wire Cable Braiding Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWhole Absorbtion Flexo Printing Soltting Die Cutting Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.200m Diesel Oil Hydraulic Water Well Drilling Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Cmyk Printing Cake Gift Packing Box with Lid, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Air Purification Activated Alumina, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

China Wholesale Oxide Aluminium Black Sand Blast Media. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Agriculture Apple Orchard Reflective Pet Film) for the financial support.

REFERENCES

Welded Stud Anchor Chain for Ship, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic Nylon Sheet with High Temperature]

Brown, E. M; Thompson, C .J.,SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, M.M.(1994),Line Scan Uvss System Portable Under Vehicle Surveillance.The Journal of the American Leather Chemists Association (JALCA)89(7)[15L Big Cooling Cylinder High Production Hard Ice Cream Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Folding Steel Manual Wheelchair with Chrome Frame. Analytical Biochemistry212,436-445[Stationary Air Cooled Permanent Magnet VSD Air Compressor China Atlas Copco VSD Air Compressor India Atlas Copco VSD Air Compressor Atlas Copco VSD]

Dalev, P.G. and Simeonova, L.S.(1992),Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel. Biotechnology letters 14,6, 531-534[China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts]

Heidemann, E.(1997),Fashion Style Lady Alloy Quartz Watch with Simple Dial.Leder 48,(5),99-104[Car Tail Light for Ranger 2015/2016]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324]

High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Rooftop HVAC Packaged Units (RTU) Amrta Manufacture.Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box. USP/SP[Automatic Bread Crumb Processing Line and Making Extruder Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Carbide Rod H6 Tc Rods]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Building Decoration Material Acrylic Dispersant for Coating: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Solid Tungsten Carbide Rods]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Precision PVC/ EVA Injection Mould for Sandals & Shoe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052]

High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor]

High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Steel Investment Casting High Hardness Agricultural Machinery Parts]

High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Supply Straight Ball Joint Bearing 02b04, Part I.Wrinkle Effect Crease UV Drying Machine UV Curing System. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plastic Packaging Tray Vacuum Forming Machine]

Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector.20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, 175-184[Top Sell Cummins K19 Engine Parts Solenoid Valve 3018453]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Samsung LTI550HN16 1920X1080P monitor screen LCD video wall]

Whitaker,J.R.(1963),Tefude Feeding and Packaging Line for Food with Tray. Analytical Chemistry35(12), 1950-1953[Electrical Bike Waterproof Power Connector]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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