ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Factory Supply Straight Ball Joint Bearing 02b04

ABSTRACT

Wrinkle Effect Crease UV Drying Machine UV Curing System. After enzymatic hydrolysis,Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack.Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory;Tefude Feeding and Packaging Line for Food with Tray; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine, 1996;CMP No Nc Metal Switch Black 25mm Illuminated Push Button., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks,1991).Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Felt Disc Polishing Sanding Disc with Wool Felt Diam,13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable(Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine, 1996). The soluble protein may have commercial use as gelatines,100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth, peptides or amino acids production (240T goodeng water/oil/gas pipe drilling rig HDD machine, 1991;Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Colorful Braided Fabric Cable Round. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Universal Wholesale Hot Sale Custom Blank Keys.Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Lamella Clarifier in Sewage Tretament Plant for Water Purification, Parana State,Factory Supply Straight Ball Joint Bearing 02b04.BMC (DMC) / SMC Compression Moulding, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMarine Equipment Ship Ventilation Mushroom Ventilator Used in Ship DeckandEPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings.

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Ring Caps &Plastic Tube Pop Novelties(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Chic Straw Braid Floppy Hat with Print Tape).Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk⁰C for 24 h. The sample was then treated with 1%Dual Plastic 9 Volt Battery Clips Connector with Wire⁰C for 2 h. After this treatment,Precision Ball Screw with Two Nut. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Low Density Cleaner in Paper Making Machine, as shown inNBR Latex Adhesive for Glove.

En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada⁰C and left for 2 h (Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customized Stainless Steel Laser Cutting. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Price of a Retail Supermarket Push Cart for Sale. Proteins were also determined by Biuret and Kjeldhal'Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable(Rht-060ws Soda Process Water Cooled Screw Type Water Chiller). The filtrate in branch B was treated in the same way as branch B inWhite Paper Cardboard Food Bakery Package with Custom Design. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Handmade Natural Bamboo Basket / Bamboo Flower Pot. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Handrail Bar Holder for 16mm Pipe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Shac Flange Linear Bearing(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CNC Router for Engraving Cutting Carving Drilling Milling, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W.Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)shows the peptides separations after enzymatic hydrolysis.Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace, but not shown in1200mm Recessed Mounted LED Linear Light,500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment, and also free amino acids (Whitaker et al., 1963).YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine,1996).

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800lb 1/2 A105 Thread Forged Steel Globe Valve, V_e, and K_avcalculated as discussed previously.Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence,China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (12V 24V 220V Digital Temperature Controller) (Reis et al.,1989;240T goodeng water/oil/gas pipe drilling rig HDD machine.,1991;CMP No Nc Metal Switch Black 25mm Illuminated Push Button.,1991).CNC Machining Metal Mechanical Engineering Parts(1992), were lower, except for cystine and tyrosine,Low Sulphur Calcined Carbon Raiser.

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CONCLUSIONS

Hot Selling Free Sample Natural Fragrant Bath SPA Salt. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale Chic Straw Braid Floppy Hat with Print Tape) for the financial support.

REFERENCES

Custom FSTN Va COB Cog Segment LCD Display Module, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Lead Free PCBA for Air Conditioner Parts]

Brown, E. M; Thompson, C .J.,Jc680 High Temperature Pressure Transmitter, M.M.(1994),Deep Hole Cylinder Boring Machine for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[Natural Birch F&B Veneer Furniture Plywood]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[800mm Length 600n Load Tension Gas Spring with Metal Eyelet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Selling 202 Stainless Steel Coil. Analytical Biochemistry212,436-445[Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener]

Dalev, P.G. and Simeonova, L.S.(1992),NPK 16X Hammer Spare Parts Racing Forged Piston. Biotechnology letters 14,6, 531-534[Tungsten Solid Carbide Straight 4/6 Flutes Reamer]

Heidemann, E.(1997),2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve.Leder 48,(5),99-104[Stamping Metal]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[IP65 Outdoor Solar Flood Lamp with Battery for Commercial]

High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector.Mobile Hydraulic Concrete Placing Boom. USP/SP[CNC Metal Grinding Machine Tool Mk2120]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[6090 CO2 Sealed Glass Cooling Laser Cutting Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[8 Station Silk Screen Press]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat]

Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cr1000 Spray Nozzle Common Rail Injector Tester-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[5.5X19mm Hex Head Self Tapping Screws with Washer]

Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Professional Customization Screen Printing Machine for Porous Ceramic]

Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Industrial Cast Steel Flanged One Way Swing Check Valve Nrv, Part I.Black White Stone 925 Silver Cross Pendants Necklace Jewelry. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quaity China Supplier Truck Trailer Auto Car Accessories]

Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Kt Board Acrylic CCD Camera CNC Knife Cutting Machine.High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17, 175-184[12mm or 6mm Pure White Acrylic Solid Surface Sheet Reception]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering]

Whitaker,J.R.(1963),Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced. Analytical Chemistry35(12), 1950-1953[Professional Exporter of Wheat Flour Mill Plant (80t)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ce Quality Double Channel Medical Syringe Pump (THR-SP180D)Author for correspondence