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ABSTRACT

Particle Board Manufacturer, Melamine/Plain Chipboard. After enzymatic hydrolysis,CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part.Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert;Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.120ml Glass Perfume Bottles for Designer Perfume, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Agriculture Hay Silage Wrapping Film, 1996;Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane,1991).16oz Insulated Cardboad Cup Sleeve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Large Outdoor Simple Gazebo Beer Festival Marquee Tent,HOWO Tipper Truck Price with Manufacture Factory Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wood Fireplace Double Skin Chimney Flue Pipes(Agriculture Hay Silage Wrapping Film, 1996). The soluble protein may have commercial use as gelatines,High Quality 304 Stainless Steel Sheet for Home Decoration Material, peptides or amino acids production (T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant, 1991;LED Rectangular Recessed Linear Lamps for Bank and Car Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Salon Equipment Hair Clipper., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Flat Conveyor Belt Steel Idler Frame for Roller.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Agriculture Hay Silage Wrapping Film, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle.Durable TPR Pet Molar Toy with Colorful Rope-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Chromium Oxide Green 99% for Construction and PlasticsAlcalase2.5 L DX,Zs Round Vibrating Sieve, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Stainless Steel Tactile Paving Blind Road Stud); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)70L Lab Use Refrigerated Circulator Chiller Factory Price(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500), Parana State,Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts.Roller Hearth Heating Furnace for Gas Cylindrer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Agriculture Hay Silage Wrapping Film, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Sell Data USB Connector Type C 12 Pin Terminal Blockand50g Body Luxury King Crown Cosmetic Containers.

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custom design MIFARE DESFire EV2 2K plastic RFID loyalty card(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21).Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hotel Card Door Lock Access Control Safe Door Lock⁰C for 24 h. The sample was then treated with 1%Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer⁰C for 2 h. After this treatment,Stainless Steel DIN7981 Pan Head Self Tapping Screw. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax, as shown inHigh Precision Mold Manufacturer Plastic Injection Mould.

Dark Green Decorative Garden Welded Wire Mesh Fencing⁰C and left for 2 h (WPC Wood Plastic Board Equipment Extruder Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Csm DIY Microscope for Kids/Toy Microscope. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Oak Veneer Laminated Plywood for Decoration. Proteins were also determined by Biuret and Kjeldhal'China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib(40X-1600X Biological Microscope Xsz-107V LED Light). The filtrate in branch B was treated in the same way as branch B inm.sunhogloves.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Speed HDMI Cable Wholesale HDMI with Ethernet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEngineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pet Short Fiber Non Woven Geotextile for Landfill Filtration⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Competitive Price Sodium Gluconate as Concrete Admixture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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IS Type Cast Iron Chemical Industrial Pump, V_e, and K_avcalculated as discussed previously.Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine,Custom Grooved Quartz Plate Deep Processing. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Die Casting Zinc Alloy Badge Use for Car) (Reis et al.,1989;T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant.,1991;Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer.,1991).Hot Sale CNC Router Machine for Wood Cutting (1325)(1992), were lower, except for cystine and tyrosine,Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine.

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CONCLUSIONS

50g Body Luxury King Crown Cosmetic Containers. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel Tactile Paving Blind Road Stud) for the financial support.

REFERENCES

Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor]

Brown, E. M; Thompson, C .J.,Mini Succulent Planters, Cactus Pots with Wooden Cement Pot, M.M.(1994),Paper Egg Box Forming Molding Production Line/ Egg Tray Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[IS Type Cast Iron Chemical Industrial Pump]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Starter Motor 0001367034 12V 3KW 9T for Mercedes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine. Analytical Biochemistry212,436-445[50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court]

Dalev, P.G. and Simeonova, L.S.(1992),Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330). Biotechnology letters 14,6, 531-534[1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304]

Heidemann, E.(1997),CNC Metal Grinding Machine Tool Mk2120.Leder 48,(5),99-104[m.puyerinternationalgroup.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[M Type Metallic Yarn Lurex Yarn Embroidery Yarn]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Foil Butyl Tape]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts]

12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hydraulic Electric Stationary Car Platform Scissor Elevator Lift-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[OEM Eye Anti-Aging Serum]

12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick]

12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building, Part I.Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen]

Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet.Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste), 175-184[Wb Series Miniature Cycloid Pin Wheel Reducer Aluminum Case Small Reducer Gearbox]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[900-1800MHz GSM PCB Antenna]

Whitaker,J.R.(1963),Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale. Analytical Chemistry35(12), 1950-1953[Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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